Fine Mapping and Candidate Gene Analysis of Two Major Quantitative Trait Loci, qFW2.1 and qFW3.1, Controlling Fruit Weight in Pepper (Capsicum annuum)
Abstract
:1. Introduction
2. Materials and Methods
2.1. Plant Materials
2.2. DNA Extraction, PCR Amplification, and Detection
2.3. QTL-Seq Analysis
2.4. QTL Mapping with InDel Marker
2.5. Transcriptome Analysis
2.6. Verification of Gene Expression Pattern Using qRT-PCR
3. Results
3.1. Phenotypic Analysis of F2
3.2. Two Major QTL Were Identified by Using QTL-Seq
3.3. Genetic Linkage Analysis Narrowed qFW2.1 and qFW3.1 Candidate Region
3.4. Differential Gene Expression Analysis between the Two Parents
3.5. Prediction and Analysis of Candidate Genes for the Fruit Weight
4. Discussion
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Trait | Population | Chr | LOD | PVE (%) | Left | Right |
---|---|---|---|---|---|---|
fruit weight without stalk | 2021SY47 | 2 | 10.60 | 12.40 | PepC2-152-3 | PepC2-156-1 |
2022SG136 | 14.43 | 14.61 | PepC2-150-2 | PepC2-154-2 | ||
2021SY47 | 3 | 12.87 | 14.97 | PepC3-12-1 | PepC3-18-1 | |
2022SG136 | 15.37 | 15.25 | PepC3-10-2 | PepC3-16-1 |
Gene ID | Functional Prediction |
---|---|
Capana02g002936 | AP2-like ethylene-responsive transcription factor ANT |
Capana02g002938 | growth-regulating factor 2-like (GRF) |
Capana02g002971 | adenylyl-sulfate kinase |
Capana02g002977 | mitochondrial outer membrane protein porin 2 |
Capana02g002983 | domain-containing protein NPY2 |
Capana02g002984 | GCN5-related N-acetyltransferase (GNAT) family protein |
Capana02g002985 | U553_DICDI UPF0553 protein OS |
Capana02g002986 | FMDA_METME Formamidase |
Capana02g002991 | unknown protein |
Capana02g003021 | probable indole-3-acetic acid-amido synthetase GH3.1 |
Capana03g000827 | unknown protein |
Capana03g000845 | NDH-DEPENDENT CYCLIC ELECTRON FLOW 1 |
Capana03g000846 | UDP-glucose 6-dehydrogenase |
Capana03g000898 | NLI interacting factor (NIF) family protein |
Capana03g000903 | C78A4_PINRA Cytochrome P450 |
Capana03g000937 | zinc finger (C2H2 type) family protein |
Capana03g000971 | phosphoglycerate/bisphosphoglycerate mutase family protein |
Capana03g000998 | ARATH Protein PRD1 |
Capana03g0001010 | gibberellin-regulated protein 14 |
Capana03g0001013 | calmodulin-binding protein |
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Guan, C.; Jin, Y.; Zhang, Z.; Cao, Y.; Wu, H.; Zhou, D.; Shao, W.; Yang, C.; Ban, G.; Ma, L.; et al. Fine Mapping and Candidate Gene Analysis of Two Major Quantitative Trait Loci, qFW2.1 and qFW3.1, Controlling Fruit Weight in Pepper (Capsicum annuum). Genes 2024, 15, 1097. https://doi.org/10.3390/genes15081097
Guan C, Jin Y, Zhang Z, Cao Y, Wu H, Zhou D, Shao W, Yang C, Ban G, Ma L, et al. Fine Mapping and Candidate Gene Analysis of Two Major Quantitative Trait Loci, qFW2.1 and qFW3.1, Controlling Fruit Weight in Pepper (Capsicum annuum). Genes. 2024; 15(8):1097. https://doi.org/10.3390/genes15081097
Chicago/Turabian StyleGuan, Congcong, Yuan Jin, Zhenghai Zhang, Yacong Cao, Huamao Wu, Daiyuan Zhou, Wenqi Shao, Chuangchuang Yang, Guoliang Ban, Lingling Ma, and et al. 2024. "Fine Mapping and Candidate Gene Analysis of Two Major Quantitative Trait Loci, qFW2.1 and qFW3.1, Controlling Fruit Weight in Pepper (Capsicum annuum)" Genes 15, no. 8: 1097. https://doi.org/10.3390/genes15081097