Expression, Purification, and Anti-UV Irradiation Effect of RsSOD on HCE-T Human Corneal Epithelial Cells
Abstract
:1. Introduction
2. Materials and Methods
2.1. Strain and Cell Line
2.2. Bioinformatics Analysis
2.3. Expression and Purification of RsSOD Protein
2.4. Stability Determination of RsSOD Protein
2.5. Effect of RsSOD Protein on the Proliferation of HCE-T Cells
2.6. Effect of Long-Term or Short-Term UV Irradiation on the Proliferation of HCE-T Cells
2.7. Effect of RsSOD on the Proliferation of HCE-T Cells under UV Irradiation
2.8. Statistical Analysis
3. Results
3.1. Bioinformatics Analysis
3.2. Expression and Purification of RsSOD Protein
3.3. Stability of RsSOD Protein
3.4. Determination of Optimal Dose of RsSOD against UV Irradiation on HCE-T Cells
3.5. Effect of RsSOD against Long-Term UV Irradiation in HCE-T Cells
3.6. Effect of RsSOD against Short-Term UV Irradiation in HCE-T Cells
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
Abbreviations
SOD | Superoxide dismutase |
MnSOD | Manganese superoxide dismutase |
RsSOD | Radiobacillus sp. SOD |
IPTG | Isopropyl β-D-1-thiogalactopyranoside |
UV | Ultraviolet |
MDA | Maleicdialdehyde |
HCE-T | Human corneal epithelial cells-transformed |
ROS | Reactive oxygen species |
ORF | Open reading frame |
mtDNA | Mitochondrial DNA |
DEAE | Diethylaminoethyl |
WST-8 | 2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium sodium salt |
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Fu, X.; Jiang, Z.; Bi, W.; Yang, Z.; Lu, W.; Chen, J.; Lyu, Z.; Nie, Z. Expression, Purification, and Anti-UV Irradiation Effect of RsSOD on HCE-T Human Corneal Epithelial Cells. Genes 2024, 15, 1147. https://doi.org/10.3390/genes15091147
Fu X, Jiang Z, Bi W, Yang Z, Lu W, Chen J, Lyu Z, Nie Z. Expression, Purification, and Anti-UV Irradiation Effect of RsSOD on HCE-T Human Corneal Epithelial Cells. Genes. 2024; 15(9):1147. https://doi.org/10.3390/genes15091147
Chicago/Turabian StyleFu, Xucong, Zhuo Jiang, Wenhui Bi, Zhecheng Yang, Weina Lu, Jianqing Chen, Zhengbing Lyu, and Zuoming Nie. 2024. "Expression, Purification, and Anti-UV Irradiation Effect of RsSOD on HCE-T Human Corneal Epithelial Cells" Genes 15, no. 9: 1147. https://doi.org/10.3390/genes15091147