Correlations between Electrophysiological Parameters, Lymphocyte Distribution and Cytokine Levels in Patients with Chronic Demyelinating Inflammatory Polyneuropathy
Abstract
:1. Introduction
2. Materials and Methods
2.1. Blood Collection and Staining for Markers of Leukocyte Subpopulations
2.2. Serum Collection, Storage and Cytokine Analysis
2.3. Electroneurography
3. Statistical Analysis
Data Availability
4. Results
5. Discussion
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Distal Latency ± SD [ms] | Conduction Velocity ± SD [m/s] | Amplitude ± SD [mV] | F-Wave [ms] | ||
---|---|---|---|---|---|
CMAP | median | 5.57 ± 3.17 | 46.84 ± 9.87 | 5.34 ± 3.42 | 36.24 ± 9.87 |
ulnar | 3.49 ± 1.48 | 48.07 ± 9.91 | 7.16 ± 2.87 | 35.15 ± 7.94 | |
peroneal | 6.14 ± 1.83 | 34.43 ± 12.27 | 1.98 ± 1.95 | 76.33 ± 13.71 | |
tibial | 6.37 ± 1.76 | 34.46 ± 11.37 | 2.96 ± 2.52 | 70.45 ± 9.29 | |
SNAP | median | 3.82 ± 0.94 | 36.90 ± 17.51 | 11.42 ± 8.97 | - |
ulnar | 3.54 ± 0.94 | 33.72 ± 17.71 | 11.08 ± 9.34 | - | |
sural | 4.31 ± 1.24 | 25.06 ± 21.55 | 4.29 ± 3.33 | - |
Leukocytes | All Patients n = 60 | Normal Results | Incorrect Results | |||
---|---|---|---|---|---|---|
Mean ± SD | n | Mean ± SD | n | Mean ± SD | ||
WBC [Cells/µL] | 6937.45 ± 2064.83 | 51 | 6645.29 ± 1422.33 | 9 | 8593.00 ± 3895.14 | |
CD45+ Leukocytes | Monocytes [%] | 8.01 ± 2.46 | 46 | 7.04 ± 1.87 | 14 | 11.21 ± 1.08 |
Monocytes cells/µL | 553.55 ± 235.37 | 54 | 513.39 ± 170.19 | 6 | 914.97 ± 415.31 | |
Lymphocytes [%] | 39.17 ± 13.72 | 36 | 33.08 ± 6.64 | 24 | 48.30 ± 16.45 | |
Lymphocytes cells/µL | 2702.10 ± 1208.41 | 45 | 2671.27 ± 723.27 | 15 | 2794.59 ± 2120.75 | |
Lymphocytes | B CD19+ Lymphocytes [%] | 11.54 ± 5.55 | 19 | 13.01 ± 1.54 | 41 | 10.86 ± 6.55 |
B CD19+ Lymphocytes cells/µL | 325.66 ± 262.29 | 50 | 290.66 ± 148.05 | 10 | 500.66 ± 539.29 | |
T CD3+ Lymphocytes [%] | 69.98 ± 10.03 | 16 | 73.98 ± 2.31 | 44 | 68.53 ± 11.32 | |
T CD3+ Lymphocytes cells/µL | 1894.47 ± 869.09 | 45 | 1670.97 ± 438.67 | 15 | 2564.97 ± 1391.45 | |
T Lymphocytes | T CD3+ CD4+ Lymphocytes [%] | 62.97 ± 12.05 | 9 | 48.69 ± 3.58 | 51 | 65.50 ± 11.23 |
T CD3+ CD4+ Lymphocytes cells/µL | 1223.41 ± 660.56 | 38 | 1026.65 ± 295.22 | 22 | 1563.28 ± 938.76 | |
T CD3+ CD8+ Lymphocytes [%] | 25.33 ± 10.09 | 10 | 32.19 ± 2.78 | 50 | 23.96 ± 10.47 | |
T CD3+ CD8+ Lymphocytes cells/µL | 461.82 ± 299.29 | 42 | 468.15 ± 153.12 | 18 | 447.06 ± 488.45 | |
CD4/CD8 | 3.16 ± 2.18 | 6 | 2.02 ± 0.12 | 54 | 3.28 ± 2.27 |
Cytokines | CIDP Patients n = 60 | Control Group n = 18 | p-Value |
---|---|---|---|
Mean ± SD [pg/mL] | Mean ± SD [pg/mL] | ||
IFN-y | 7.72 ± 1.31 | 7.29 ± 1.96 | 0.590 |
TNF-α | 10.04 ± 11.12 | 9.99 ± 2.92 | 0.009 |
IL-10 | 8.15 ± 1.12 | 7.77 ± 1.75 | 0.524 |
IL-6 | 10.18 ± 4.44 | 8.33 ± 2.65 | 0.019 |
IL-4 | 7.98 ± 0.98 | 7.40 ± 1.33 | 0.047 |
IL-2 | 7.35 ± 0.83 | 6.54 ± 0.85 | 0.0006 |
CMAP Amplitude | SNAP Amplitude | F-Wave | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Median | Ulnar | Peroneal | Tibial | Median | Ulnar | Sural | Median | Ulnar | Peroneal | Tibial | |||
Lymphocytes | Monocytes | [%] | −0.17 | ||||||||||
cells/µL | −0.11 | −0.23 | −0.19 | 0.12 | |||||||||
Lymphocytes | [%] | 0.32 | −0.18 | −0.28 | −0.14 | −0.12 | |||||||
cells/µL | −0.13 | −0.17 | −0.19 | −0.14 | |||||||||
B CD19+ Lymphocytes | [%] | 0.24 | −0.14 | −0.24 | −0.19 | 0.14 | 0.14 | 0.13 | |||||
cells/µL | 0.26 | −0.14 | −0.21 | −0.12 | |||||||||
T CD3+ Lymphocytes | [%] | 0.16 | 0.22 | 0.28 | 0.27 | 0.11 | −0.20 | −0.17 | −0.20 | −0.31 | |||
cells/µL | −0.15 | −0.22 | −0.17 | −0.13 | −0.16 | ||||||||
TCD3+ CD4+ Lymphocytes | [%] | 0.16 | −0.14 | −0.14 | −0.14 | ||||||||
cells/µL | 0.11 | −0.11 | −0.16 | −0.19 | −0.11 | −0.17 | |||||||
TCD3+ CD8+ Lymphocytes | [%] | 0.12 | |||||||||||
cells/µL | 0.13 | 0.14 | −0.15 | −0.26 | |||||||||
Cytokines | IFN-y | −0.24 | −0.19 | −0.30 | 0.25 | −0.13 | −0.24 | ||||||
TNF-α | −0.20 | −0.25 | −0.16 | −0.18 | |||||||||
IL-10 | −0.13 | −0.32 | −0.28 | 0.13 | −0.12 | ||||||||
IL-6 | −0.25 | −0.17 | −0.24 | −0.15 | −0.13 | −0.20 | |||||||
IL-4 | −0.30 | −0.21 | 0.19 | −0.24 | |||||||||
IL-2 | −0.24 | 0.14 | 0.27 | −0.37 | −0.22 | −0.12 |
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Dziadkowiak, E.; Moreira, H.; Wieczorek, M.; Budrewicz, S.; Barg, E.; Koszewicz, M. Correlations between Electrophysiological Parameters, Lymphocyte Distribution and Cytokine Levels in Patients with Chronic Demyelinating Inflammatory Polyneuropathy. J. Pers. Med. 2021, 11, 766. https://doi.org/10.3390/jpm11080766
Dziadkowiak E, Moreira H, Wieczorek M, Budrewicz S, Barg E, Koszewicz M. Correlations between Electrophysiological Parameters, Lymphocyte Distribution and Cytokine Levels in Patients with Chronic Demyelinating Inflammatory Polyneuropathy. Journal of Personalized Medicine. 2021; 11(8):766. https://doi.org/10.3390/jpm11080766
Chicago/Turabian StyleDziadkowiak, Edyta, Helena Moreira, Malgorzata Wieczorek, Slawomir Budrewicz, Ewa Barg, and Magdalena Koszewicz. 2021. "Correlations between Electrophysiological Parameters, Lymphocyte Distribution and Cytokine Levels in Patients with Chronic Demyelinating Inflammatory Polyneuropathy" Journal of Personalized Medicine 11, no. 8: 766. https://doi.org/10.3390/jpm11080766