WGCNA-Based DNA Methylation Profiling Analysis on Allopurinol-Induced Severe Cutaneous Adverse Reactions: A DNA Methylation Signature for Predisposing Drug Hypersensitivity
Abstract
:1. Introduction
2. Materials and Methods
2.1. Patients
2.2. Blood Sample Collection, Genomic DNA Extraction, and Methylation Beadchip Assay
2.3. Analysis Software
2.4. Screening Differentially Methylated Positions and Regions
2.5. Algorithm of Weighted Gene Co-Expression Network Analysis (WGCNA)
2.6. The Definition of Dissimilarity Measurement in WGCNA
3. Results
3.1. The Methylation Array Data Were Quality Controlled and Normalized
3.2. Screening Differentially Methylated Positions and Regions
3.3. Selection of Parameters for WGCNA
3.4. Hub Genes Selection
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Cheng, L.; Sun, B.; Xiong, Y.; Hu, L.; Gao, L.; Li, J.; Xie, H.; Chen, X.; Zhang, W.; Zhou, H.-H. WGCNA-Based DNA Methylation Profiling Analysis on Allopurinol-Induced Severe Cutaneous Adverse Reactions: A DNA Methylation Signature for Predisposing Drug Hypersensitivity. J. Pers. Med. 2022, 12, 525. https://doi.org/10.3390/jpm12040525
Cheng L, Sun B, Xiong Y, Hu L, Gao L, Li J, Xie H, Chen X, Zhang W, Zhou H-H. WGCNA-Based DNA Methylation Profiling Analysis on Allopurinol-Induced Severe Cutaneous Adverse Reactions: A DNA Methylation Signature for Predisposing Drug Hypersensitivity. Journal of Personalized Medicine. 2022; 12(4):525. https://doi.org/10.3390/jpm12040525
Chicago/Turabian StyleCheng, Lin, Bao Sun, Yan Xiong, Lei Hu, Lichen Gao, Ji Li, Hongfu Xie, Xiaoping Chen, Wei Zhang, and Hong-Hao Zhou. 2022. "WGCNA-Based DNA Methylation Profiling Analysis on Allopurinol-Induced Severe Cutaneous Adverse Reactions: A DNA Methylation Signature for Predisposing Drug Hypersensitivity" Journal of Personalized Medicine 12, no. 4: 525. https://doi.org/10.3390/jpm12040525