Construction of Osteosarcoma Diagnosis Model by Random Forest and Artificial Neural Network
Abstract
:1. Introduction
2. Materials and Methods
2.1. Database from GEO and Analysis
2.2. Differentially Expressed Genes and Enrichment Investigation
2.3. Random Forest Analysis for DEGs and Visualization
2.4. Construction of Artificial Neural Network Model
2.5. Validation of the Artificial Neural Network Model
2.6. Immune Infiltration Analysis
2.7. Cox Proportional Hazards Model and Survival Analysis
3. Results
3.1. DEG Identification
3.2. Metascape Analysis Resource of DEGs
3.3. Analysis of GO Enrichment and KEGG Pathways
3.4. Random Forest Tree Screening
3.5. Construction and Validation of the Osteosarcoma-Related Gene Diagnostic Model
3.6. Immune Infiltration Analysis
3.7. Cox Proportional Hazards Model and Survival Analysis
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
Abbreviations
AASS | Aminoadipate Semialdehyde Synthase |
ANK1 | Ankyrin 1 |
ANN | Artificial neural network |
AUC | Area Under Curves |
DEG | differentially expressed gene |
ECM | Extracellular matrix |
GEO | Gene Expression Omnibus |
GO | Gene Ontology |
GREM2 | Gremlin 2, DAN Family BMP Antagonist |
HSPB8 | Heat Shock Protein Family B (Small) Member 8 |
ITGA7 | Integrin Subunit Alpha 7 |
KEGG | Kyoto Encyclopedia of Genes |
MRI | Magnetic Resonance Imaging |
NFASC | Neurofascin |
NK | natural killer |
OS | Osteosarcoma |
RHD | Rh Blood Group D Antigen |
ROC | receiver operating characteristic |
TARGET | Therapeutically Applicable Research To Generate Effective Treatments |
TGFBR3 | Transforming Growth Factor Beta Receptor 3 |
TNFRSF21 | TNF Receptor Superfamily Member 21 |
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DataSets Series | Platform | Tumor | Normal | Group |
---|---|---|---|---|
GSE14359 | GPL96 | 10 | 2 | Training group |
GSE99671 | GPL20148 | 18 | 18 | Training group |
GSE126209 | GPL20301 | 11 | 12 | Training group |
GSE19276 | GPL6848 | 23 | 5 | Validation group |
Group | Normal | OS | Total |
---|---|---|---|
Normal | 32 | 0 | 32 |
OS | 0 | 39 | 39 |
Group | Normal | OS | Total |
---|---|---|---|
Normal | 5 | 0 | 5 |
OS | 4 | 19 | 23 |
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Li, S.; Que, Y.; Yang, R.; He, P.; Xu, S.; Hu, Y. Construction of Osteosarcoma Diagnosis Model by Random Forest and Artificial Neural Network. J. Pers. Med. 2023, 13, 447. https://doi.org/10.3390/jpm13030447
Li S, Que Y, Yang R, He P, Xu S, Hu Y. Construction of Osteosarcoma Diagnosis Model by Random Forest and Artificial Neural Network. Journal of Personalized Medicine. 2023; 13(3):447. https://doi.org/10.3390/jpm13030447
Chicago/Turabian StyleLi, Sheng, Yukang Que, Rui Yang, Peng He, Shenglin Xu, and Yong Hu. 2023. "Construction of Osteosarcoma Diagnosis Model by Random Forest and Artificial Neural Network" Journal of Personalized Medicine 13, no. 3: 447. https://doi.org/10.3390/jpm13030447