The Genome-Wide DNA Methylation Profile of Peripheral Blood Is Not Systematically Changed by Short-Time Storage at Room Temperature
Abstract
:1. Introduction
2. Results
2.1. High Quality Sequencing Data Generated from Time Points up to Three Days
2.2. No Storage-Dependent Change in Canonical Methylation Patterns
2.3. Stochastic Variation between Time-Points and No Differently Methylated Regions between Groups
3. Discussion
4. Materials and Methods
4.1. Blood Samples
4.2. MeDIP-seq
4.3. Sequencing and Bioinformatics
4.4. Pyrosequencing
4.5. Ethics Statement
4.6. Availability of Data and Materials
Supplementary Materials
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Staunstrup, N.H.; Starnawska, A.; Nyegaard, M.; Nielsen, A.L.; Børglum, A.D.; Mors, O. The Genome-Wide DNA Methylation Profile of Peripheral Blood Is Not Systematically Changed by Short-Time Storage at Room Temperature. Epigenomes 2017, 1, 23. https://doi.org/10.3390/epigenomes1030023
Staunstrup NH, Starnawska A, Nyegaard M, Nielsen AL, Børglum AD, Mors O. The Genome-Wide DNA Methylation Profile of Peripheral Blood Is Not Systematically Changed by Short-Time Storage at Room Temperature. Epigenomes. 2017; 1(3):23. https://doi.org/10.3390/epigenomes1030023
Chicago/Turabian StyleStaunstrup, Nicklas Heine, Anna Starnawska, Mette Nyegaard, Anders Lade Nielsen, Anders Dupont Børglum, and Ole Mors. 2017. "The Genome-Wide DNA Methylation Profile of Peripheral Blood Is Not Systematically Changed by Short-Time Storage at Room Temperature" Epigenomes 1, no. 3: 23. https://doi.org/10.3390/epigenomes1030023