Silver-Doped Anodic Alumina with Antimicrobial Properties—Synthesis and Characterization
Abstract
:1. Introduction
2. Materials and Methods
2.1. Silver-Doped Anodic Alumina Preparation
2.2. Microstructure Characterization of the Modified Structures
2.3. Evaluation of Antibacterial Activity
3. Results and Discussion
3.1. Scanning Electron Microscope Observations
3.2. Antibacterial Activity of Silver-Modified Alumina Substrates
3.3. Silver-Modified Alumina Substrates—Comparison of Biocompatibility
4. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
- Yang, R.; Sui, C.; Gong, J.; Qu, L. Silver nanowires prepared by modified AAO template method. Mater. Lett. 2007, 61, 900–903. [Google Scholar] [CrossRef]
- Gultepe, E.; Nagesha, D.; Sridhar, S.; Amiji, M. Nanoporous inorganic membranes or coatings for sustained drug delivery in implantable devices. Adv. Drug Deliv. Rev. 2010, 62, 305–315. [Google Scholar] [CrossRef] [PubMed]
- Sedel, L. Evolution of alumina-on-alumina implants: A review. Clin. Orthop. Relat. Res. 2000, 379, 48–54. [Google Scholar] [CrossRef] [PubMed]
- Toccafondi, C.; Dante, S.; Reverberi, A.P.; Salerno, M. Biomedical applications of anodic porous alumina. Curr. Nanosci. 2015, 11, 572–580. [Google Scholar] [CrossRef]
- Kusy, R.P. Orthodontic Biomaterials: From the Past to the Present. Angle Orthod. 2002, 72, 501–512. [Google Scholar] [CrossRef]
- Poinern, G.E.J.; Ali, N.; Fawcett, D. Progress in nano-engineered anodic aluminum oxide membrane development. Materials 2011, 4, 487–526. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Calovi, M.; Furlan, B.; Coroneo, V.; Massidda, O.; Rossi, S. Facile Route to Effective Antimicrobial Aluminum Oxide Layer Realized by Co-Deposition with Silver Nitrate. Coatings 2022, 12, 28. [Google Scholar] [CrossRef]
- Dehghan, F.; Mardanpour, H.; Kamali, S.; Alirezaei, S. Synthesis and antibacterial properties of novel Al2O3-Ag anodised composite coating. Mater. Technol. 2021, 36, 721–730. [Google Scholar] [CrossRef]
- Tzaneva, B.R. Electrochemical and Electroless Deposition of Metal in Anodic Aluminium Oxide Nanoporous Template. Annu. J. Electron. 2013, 204–206. [Google Scholar]
- Girginov, C.; Kozhukharov, S.; Kiradzhiyska, D.; Mancheva, R. Characterization of porous anodic alumina with AC-incorporated silver. Electrochim. Acta 2018, 292, 614–627. [Google Scholar] [CrossRef]
- Hermann, E. Elektrolytisches Färben von Anodisiertem Aluminium. Galvanotechnik 1972, 63, 110–121. [Google Scholar]
- Jagminas, A.; Žalnėravičius, R.; Rėza, A.; Paškevičius, A.; Selskienė, A. Design, optical and antimicrobial properties of extremely thin alumina films colored with silver nanospecies. Dalton Trans. 2015, 44, 4512–4519. [Google Scholar] [CrossRef]
- Chi, G.J.; Yao, S.W.; Fan, J.; Zhang, W.G.; Wang, H.Z. Antibacterial activity of anodized aluminum with deposited silver. Surf. Coat. Technol. 2002, 157, 162–165. [Google Scholar] [CrossRef]
- Mokhena, T.C.; Luyt, A.S. Electrospun alginate nanofibres impregnated with silver nanoparticles: Preparation, morphology and antibacterial properties. Carbohydr. Polym. 2017, 165, 304–312. [Google Scholar] [CrossRef] [PubMed]
- Ying, J.Y. The era of nanotechnology. Nano Today 2008, 3, 1. [Google Scholar] [CrossRef]
- Marambio-Jones, C.; Hoek, E.M.V. A review of the antibacterial effects of silver nanomaterials and potential implications for human health and the environment. J. Nanopart. Res. 2010, 12, 1531–1551. [Google Scholar] [CrossRef]
- Zhang, X.-F.; Liu, Z.-G.; Shen, W.; Gurunathan, S. Silver nanoparticles: Synthesis, characterization, properties, applications, and therapeutic approaches. Int. J. Mol. Sci. 2016, 17, 1534. [Google Scholar] [CrossRef]
- Dhand, V.; Soumya, L.; Bharadwaj, S.; Chakra, S.; Bhatt, D.; Sreedhar, B. Green synthesis of silver nanoparticles using Coffea arabica seed extract and its antibacterial activity. Mater. Sci. Eng. C 2016, 58, 36–43. [Google Scholar] [CrossRef]
- Singh, A.; Kaur, K. Biological and physical applications of silver nanoparticles with emerging trends of green synthesis. In Engineered Nanomaterials-Health and Safety; InTech Open: London, UK, 2019. [Google Scholar]
- Thorat, S.; Diaspro, A.; Scarpellini, A.; Povia, M.; Salerno, M. Comparative study of loading of anodic porous alumina with silver nanoparticles using different methods. Materials 2013, 6, 206–216. [Google Scholar] [CrossRef] [Green Version]
- Kędziora, A.; Speruda, M.; Krzyżewska, E.; Rybka, J.; Łukowiak, A.; Bugla-Płoskońska, G. Similarities and differences between silver ions and silver in nanoforms as antibacterial agents. Int. J. Mol. Sci. 2018, 19, 444. [Google Scholar] [CrossRef] [Green Version]
- Mosmann, T. Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays. J. Immunol. Methods 1983, 65, 55–63. [Google Scholar] [CrossRef]
- Moyo, B.; Mukanganyama, S. Antibacterial effects of Cissus welwitschii and Triumfetta welwitschii extracts against Escherichia coli and Bacillus cereus. Int. J. Bacteriol. 2015, 2015, 162028. [Google Scholar] [CrossRef]
- Zhang, D.; Ren, L.; Zhang, Y.; Xue, N.; Yang, K.; Zhong, M. Antibacterial activity against Porphyromonas gingivalis and biological characteristics of antibacterial stainless steel. Colloids Surf. B Biointerfaces 2013, 105, 51–57. [Google Scholar] [CrossRef] [PubMed]
- Kiradzhiyska, D.; Batsalova, T.; Dzhambazov, B.; Mancheva, R. In vitro Biocompatibility Evaluation of Anodic Alumina Substrates with Electrochemically Embedded Silver. Rev. Chim. 2020, 71, 81–88. [Google Scholar] [CrossRef]
- Ofoegbu, S.U.; Fernandes, F.A.O.; Pereira, A.B. The sealing step in aluminum anodizing: A focus on sustainable strategies for enhancing both energy efficiency and corrosion resistance. Coatings 2020, 10, 226. [Google Scholar] [CrossRef] [Green Version]
- Pornnumpa, N.; Jariyaboon, M. Antibacterial and Corrosion Resistance Properties of Anodized AA6061 Aluminum Alloy. Eng. J. 2019, 23, 171–181. [Google Scholar] [CrossRef]
- Kiradzhiyska, D.; Milcheva, N.; Mancheva, R.; Batsalova, T.; Dzhambazov, B.; Zahariev, N. Preparation and Preliminary Evaluation of Silver-Modified Anodic Alumina for Biomedical Applications. Metals 2022, 12, 51. [Google Scholar] [CrossRef]
- Lin, J.-J.; Lin, W.-C.; Li, S.-D.; Lin, C.-Y.; Hsu, S. Evaluation of the antibacterial activity and biocompatibility for silver nanoparticles immobilized on nano silicate platelets. ACS Appl. Mater. Interfaces 2013, 5, 433–443. [Google Scholar] [CrossRef]
- Martínez-Castañón, G.A.; Niño-Martínez, N.; Martínez-Gutierrez, F.; Martínez-Mendoza, J.R.; Ruiz, F. Synthesis and antibacterial activity of silver nanoparticles with different sizes. J. Nanopart. Res. 2008, 10, 1343–1348. [Google Scholar] [CrossRef]
- Maddinedi, S.B.; Mandal, B.K.; Anna, K.K. Environment friendly approach for size controllable synthesis of biocompatible Silver nanoparticles using diastase. Environ. Toxicol. Pharmacol. 2017, 49, 131–136. [Google Scholar] [CrossRef]
- Mohamed, D.S.; Abd El-Baky, R.M.; Sandle, T.; Mandour, S.A.; Ahmed, E.F. Antimicrobial Activity of Silver-Treated Bacteria against other Multi-Drug Resistant Pathogens in Their Environment. Antibiotics 2020, 9, 181. [Google Scholar] [CrossRef] [PubMed] [Green Version]
Sample Groups Abbreviation | Corresponding Surface Treatments | ||
---|---|---|---|
Electrochemical Oxidation Time, (min) | Silver Deposition Time, (min) | Silver Deposition Method | |
A | 30 | 0 | Not conducted |
B | 60 | 0 | Not conducted |
AT-3 | 30 | 3 | Thermal reduction (electroless method) |
AT-6 | 30 | 6 | |
BT-3 | 60 | 3 | |
BT-6 | 60 | 6 | |
AE-0.5 | 30 | 0.5 | Electrochemical method |
AE-8 | 30 | 8 | |
BE-0.5 | 60 | 0.5 | |
BE-8 | 60 | 8 |
Sample Groups | Electrochemical Oxidation Time, (min) | Post-Anodization Sealing Procedure | Anodic Film Thickness, (µm) |
---|---|---|---|
A | 30 | not conducted | 9.67 ± 0.26 |
A | 30 | conducted | 17.31 ± 0.31 |
B | 60 | not conducted | 17.74 ± 0.38 |
B | 60 | conducted | 24.98 ± 0.28 |
Specimens | Antibacterial Rate, % | |
---|---|---|
E. coli | B. cereus | |
AT-3 | 0 | 10.7 |
AT-6 | 14 | 20 |
BT-3 | 0 | 6 |
BT-6 | 4.6 | 16 |
AE-0.5 | 100 | 100 |
AE-8 | 100 | 100 |
BE-0.5 | 100 | 100 |
BE-8 | 100 | 100 |
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Kiradzhiyska, D.; Milcheva, N.; Batsalova, T.; Dzhambazov, B.; Zahariev, N.; Mancheva, R. Silver-Doped Anodic Alumina with Antimicrobial Properties—Synthesis and Characterization. Metals 2022, 12, 474. https://doi.org/10.3390/met12030474
Kiradzhiyska D, Milcheva N, Batsalova T, Dzhambazov B, Zahariev N, Mancheva R. Silver-Doped Anodic Alumina with Antimicrobial Properties—Synthesis and Characterization. Metals. 2022; 12(3):474. https://doi.org/10.3390/met12030474
Chicago/Turabian StyleKiradzhiyska, Denitsa, Nikolina Milcheva, Tsvetelina Batsalova, Balik Dzhambazov, Nikolay Zahariev, and Rositsa Mancheva. 2022. "Silver-Doped Anodic Alumina with Antimicrobial Properties—Synthesis and Characterization" Metals 12, no. 3: 474. https://doi.org/10.3390/met12030474