Anti-Sarcopenic Obesity Effects of Lonicera caerulea Extract in High-Fat Diet-Fed Mice
Abstract
:1. Introduction
2. Materials and Methods
2.1. Preparation and HPLC Analysis of HB Extract
2.2. Animals and Treatment
2.3. Biochemical Assays
2.4. Histological Assay
2.5. Micro-Computed Tomography (Micro-CT) and Muscle Strength
2.6. Real-Time PCR Analysis
2.7. Statistical Analysis
3. Results
3.1. Detection of C3G Contents in Raw HB and HB Extract
3.2. Effects of HB on Body Weights and Serum Biochemical Parameters
3.3. Effects of HB on Fat Accumulation by Micro-CT Analysis
3.4. Effects of HB on Antioxidant Enzymes
3.5. Effects of HB on Muscle Mass by Micro-CT Analysis
3.6. Effects of HB on Muscle Mass and Expression Levels of Atrophy Related Genes
3.7. Effects of HB on Growth Related Genes Expression
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Genes | Accession Number | Forward Primer (5′–3′) | Reverse Primer (5′–3′) |
---|---|---|---|
Sod1 | NM_011434.2 | GTGATTGGGATTGCGCAGTA | TGGTTTGAGGGTAGCAGATGAGT |
Gpx1 | U13705.1 | GAAGAACTTGGGCCATTTGG | TCTCGCCTGGCTCCTGTTT |
Cat | NM_009804.2 | TGAGAAGCCTAAGAACGCAA | CCCTTCGCAGCCATGTG |
MuRF1 | NM_001039048.2 | GACAGTCGCATTTCAAAGCA | AGGGATTCGCAGCCTGGAAG |
Atrogin1 | NM_026346.3 | CAGCTTCGTGAGCGACCTC | GGCAGTCGAGAAGTCCAGTC |
Ppargc1a | NM_008904.2 | ATGTGTCGCCTTCTTGCTCT | ATCTACTGCCTGGGGACCTT |
Sirt1 | NM_019812.3 | GCTGACGACTTCGACGACG | TCGGTCAACAGGAGGTTGTCT |
Actb | NM_007393.5 | CCACAGCTGAGAGGGAAATC | AAGGAAGGCTGGAAAAGAGC |
Rn18s | NR_003278.3 | AGCCTGAGAAACGGCTACC | TCCCAAGATCCAACTACGAG |
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Lee, Y.-S.; Park, E.-J.; Kim, S.-M.; Kim, J.-Y.; Lee, H.-J. Anti-Sarcopenic Obesity Effects of Lonicera caerulea Extract in High-Fat Diet-Fed Mice. Antioxidants 2021, 10, 1633. https://doi.org/10.3390/antiox10101633
Lee Y-S, Park E-J, Kim S-M, Kim J-Y, Lee H-J. Anti-Sarcopenic Obesity Effects of Lonicera caerulea Extract in High-Fat Diet-Fed Mice. Antioxidants. 2021; 10(10):1633. https://doi.org/10.3390/antiox10101633
Chicago/Turabian StyleLee, You-Suk, Eun-Jung Park, Sung-Min Kim, Jong-Yeon Kim, and Hae-Jeung Lee. 2021. "Anti-Sarcopenic Obesity Effects of Lonicera caerulea Extract in High-Fat Diet-Fed Mice" Antioxidants 10, no. 10: 1633. https://doi.org/10.3390/antiox10101633