Antioxidant Compound, Oxyresveratrol, Inhibits APP Production through the AMPK/ULK1/mTOR-Mediated Autophagy Pathway in Mouse Cortical Astrocytes
Abstract
:1. Introduction
2. Materials and Methods
2.1. Reagents
2.2. Cortical Astrocyte Culture
2.3. Cortical Neuron Cultures
2.4. Immunocytochemical Analysis
2.5. ULK1 Small Interfering RNA (siRNA) Transfection
2.6. Immunoblotting
2.7. Autophagic Flux Evaluation
2.8. Statistical Analysis
3. Results
3.1. OxyR Treatment Activates Autophagic Flux in Mouse Cortical Astrocytes and Rat Cortical Neurons
3.2. OxyR Activates the Autophagic Pathway in Mouse Cortical Astrocytes and Rat Cortical Neurons
3.3. OxyR-Mediated Autophagy Is Dependent on the AMPK-mTOR Signaling Pathway in Mouse Cortical Astrocytes
3.4. OxyR Activates the Autophagy Initiation Protein ULK1 in Mouse Cortical Astrocytes
3.5. OxyR Activates Lysosomal Protein LAMP1 in Mouse Cortical Astrocytes
3.6. OxyR Decreases Glucocorticoid-Induced APP Expression via the Autophagy Pathway in Mouse Cortical Astrocytes
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Rahman, M.A.; Cho, Y.; Nam, G.; Rhim, H. Antioxidant Compound, Oxyresveratrol, Inhibits APP Production through the AMPK/ULK1/mTOR-Mediated Autophagy Pathway in Mouse Cortical Astrocytes. Antioxidants 2021, 10, 408. https://doi.org/10.3390/antiox10030408
Rahman MA, Cho Y, Nam G, Rhim H. Antioxidant Compound, Oxyresveratrol, Inhibits APP Production through the AMPK/ULK1/mTOR-Mediated Autophagy Pathway in Mouse Cortical Astrocytes. Antioxidants. 2021; 10(3):408. https://doi.org/10.3390/antiox10030408
Chicago/Turabian StyleRahman, Md. Ataur, Yoonjeong Cho, Ghilsoo Nam, and Hyewhon Rhim. 2021. "Antioxidant Compound, Oxyresveratrol, Inhibits APP Production through the AMPK/ULK1/mTOR-Mediated Autophagy Pathway in Mouse Cortical Astrocytes" Antioxidants 10, no. 3: 408. https://doi.org/10.3390/antiox10030408
APA StyleRahman, M. A., Cho, Y., Nam, G., & Rhim, H. (2021). Antioxidant Compound, Oxyresveratrol, Inhibits APP Production through the AMPK/ULK1/mTOR-Mediated Autophagy Pathway in Mouse Cortical Astrocytes. Antioxidants, 10(3), 408. https://doi.org/10.3390/antiox10030408