17-β Estradiol Rescued Immature Rat Brain against Glutamate-Induced Oxidative Stress and Neurodegeneration via Regulating Nrf2/HO-1 and MAP-Kinase Signaling Pathway
Abstract
:1. Introduction
2. Materials and Methods
2.1. Chemicals
2.2. Animals and Drug Treatment
- (1)
- Control group (treated subcutaneously with 0.9% saline as a vehicle)
- (2)
- Glu group (treated subcutaneously with 10 mg/kg of glutamate)
- (3)
- Glu + E2 group (co-treated subcutaneously with 10 mg/kg of glutamate plus 10 mg/kg of 17β-estradiol).
- (4)
- E2 group (treated subcutaneously with 10 mg/kg of 17β-estradiol)
2.3. Brain Tissue Collection/Sample Preparation
2.4. Western Blot Analysis
2.5. Immunofluorescence
2.6. Antibodies
2.7. GSH Assays
2.8. Reactive Oxygen Species (ROS) Assay
2.9. Lipid Peroxidation (LPO) Assay
2.10. Statistical Analyses
3. Results
3.1. 17β-Estradiol Reduced Glutamate-Induced Oxidative Stress by Activating Nrf2/HO-1 Pathway and Enhanced Cellular Glutathione Stores in Postnatal Rat Brain
3.2. 17β-Estradiol Alleviates Glutamate Induced Neuroinflammation in Developing Rat Brain
3.3. 17β-Estradiol Abrogates Glutamate-Induced Synaptic Dysfunction in Postnatal Rat Brain
3.4. 17β-Estradiol Treatment Overcame Glutamate-Induced Neurodegeneration in Postnatal Rat Brain
3.5. 17β-Estradiol Protect Developing Rat Brain against Glutamate-Induced Excitotoxicity through Regulating P-JNK/P38 and Erk1/2 MAPK Signaling Pathways
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
Abbreviations
OS | Oxidative stress |
ROS | Reactive oxygen species |
E2 | Estradiol |
Glu | Glutamate |
LPO | Lipid peroxidation |
Nrf2 | Nuclear factor erythroid 2-related factor 2 |
HO-1 | Heme oxygenase-1 |
p-JNK | c-Jun n-terminal kinase |
P-p38 | phosphorylated p38 |
P-Erk1/2 | Extracellular signal-regulated kinase 1 and 2 |
GFAP | Glial fibrillary acidic protein |
Iba-1 | Ionized calcium-binding adaptor molecule 1 |
TNF-α | Tumor necrosis factor alpha |
Bax | Bcl-2-associated X protein |
Caspas3 | cysteine-aspartic acid protease 3 |
Bcl-2 | B-cell lymphoma 2 |
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Antibody | Catalog/Product # | Application (Conc.) | Host | Manufacturer |
---|---|---|---|---|
β-Actin | SC-47,778 | WB (1:1000) | Mouse | Santa Cruz Biotech |
Nrf2 | SC-722 | WB/IF (1:1000) | = | = |
HO1 | SC-136,961 | WB/IF (1:1000/1:100) | = | = |
GFAP | SC-33,673 | WB/IF (1:1000/1:100) | = | = |
Iba-1 | SC-32,725 | WB (1:1000) | = | = |
Iba-1 | PA5-27,436 | IF (1:100) | Rabbit | Thermo Fisher |
TNF-α | SC-52,746 | WB (1:1000) | Mouse | Santa Cruz Biotech |
PSD-95 | SC-71,933 | WB (1:1000) | = | = |
SYP | SC-17,750 | WB (1:1000) | = | = |
Bax | 2772S | WB (1:1000) | Rabbit | Cell Signaling |
Bcl-2 | SC-7382 | WB (1:1000) | Mouse | Santa Cruz Biotech |
Caspase-3 | SC-7272 | WB (1:1000) | = | = |
Caspase-3 | 9661S | IF (1:100) | Rabbit | Cell Signaling |
P-JNK | SC-6254 | WB/IF (1:1000/1:100) | Mouse | Santa Cruz Biotech |
JNK | SC-7345 | WB (1:1000) | = | = |
P-p38 | #9212 | WB (1:10,000) | Rabbit | Cell Signaling |
p38 | #9211s | WB (1:1000) | = | = |
P-Erk1/2 | #9101 | WB (1:10,000) | = | = |
Erk1/2 | 9102S | WB (1:10,000) | = | = |
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Khan, I.; Saeed, K.; Jo, M.G.; Kim, M.O. 17-β Estradiol Rescued Immature Rat Brain against Glutamate-Induced Oxidative Stress and Neurodegeneration via Regulating Nrf2/HO-1 and MAP-Kinase Signaling Pathway. Antioxidants 2021, 10, 892. https://doi.org/10.3390/antiox10060892
Khan I, Saeed K, Jo MG, Kim MO. 17-β Estradiol Rescued Immature Rat Brain against Glutamate-Induced Oxidative Stress and Neurodegeneration via Regulating Nrf2/HO-1 and MAP-Kinase Signaling Pathway. Antioxidants. 2021; 10(6):892. https://doi.org/10.3390/antiox10060892
Chicago/Turabian StyleKhan, Ibrahim, Kamran Saeed, Min Gi Jo, and Myeong Ok Kim. 2021. "17-β Estradiol Rescued Immature Rat Brain against Glutamate-Induced Oxidative Stress and Neurodegeneration via Regulating Nrf2/HO-1 and MAP-Kinase Signaling Pathway" Antioxidants 10, no. 6: 892. https://doi.org/10.3390/antiox10060892
APA StyleKhan, I., Saeed, K., Jo, M. G., & Kim, M. O. (2021). 17-β Estradiol Rescued Immature Rat Brain against Glutamate-Induced Oxidative Stress and Neurodegeneration via Regulating Nrf2/HO-1 and MAP-Kinase Signaling Pathway. Antioxidants, 10(6), 892. https://doi.org/10.3390/antiox10060892