Acylated Ghrelin Receptor Agonist HM01 Decreases Lean Body and Muscle Mass, but Unacylated Ghrelin Protects against Redox-Dependent Sarcopenia
Abstract
:1. Introduction
2. Materials and Methods
2.1. Animal Care
2.2. HM01 and Unacylated Ghrelin Delivery
2.3. Assessment of Body Composition
2.4. In Vitro Contractile Properties and Force Generation
2.5. Western Blot
2.6. Statistical Analysis
3. Results
3.1. HM01 Treatment Transiently Increases Food Intake, but Leads to Decreases in Lean Body Mass and Muscle Quantity
3.2. Longer Term Treatment of HM01 Results in Loss of Muscle Mass and Reduced Maximum Force Generation
3.3. GHSR1a Receptor Downstream Pathways and Mechanisms Are Involved in Myogenesis and Protein Synthesis in Muscle
3.4. Unacylated Ghrelin Increases Muscle Mass without Changes in Food Consumption or Body Weights
3.5. Unacylated Ghrelin Improves Contractile Properties
3.6. Downstream Pathways Activated by Unacylated Ghrelin
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Ranjit, R.; Van Remmen, H.; Ahn, B. Acylated Ghrelin Receptor Agonist HM01 Decreases Lean Body and Muscle Mass, but Unacylated Ghrelin Protects against Redox-Dependent Sarcopenia. Antioxidants 2022, 11, 2358. https://doi.org/10.3390/antiox11122358
Ranjit R, Van Remmen H, Ahn B. Acylated Ghrelin Receptor Agonist HM01 Decreases Lean Body and Muscle Mass, but Unacylated Ghrelin Protects against Redox-Dependent Sarcopenia. Antioxidants. 2022; 11(12):2358. https://doi.org/10.3390/antiox11122358
Chicago/Turabian StyleRanjit, Rojina, Holly Van Remmen, and Bumsoo Ahn. 2022. "Acylated Ghrelin Receptor Agonist HM01 Decreases Lean Body and Muscle Mass, but Unacylated Ghrelin Protects against Redox-Dependent Sarcopenia" Antioxidants 11, no. 12: 2358. https://doi.org/10.3390/antiox11122358