Multi-Omics Analysis to Understand the Effects of Dietary Proanthocyanidins on Antioxidant Capacity, Muscle Nutrients, Lipid Metabolism, and Intestinal Microbiota in Cyprinus carpio
Abstract
:1. Introduction
2. Materials and Methods
2.1. Experimental Design and Sampling
2.2. Measurement of Antioxidant Parameters
2.3. Determination of Amino Acids and Fatty Acids in Muscle
2.4. Non-Targeted Metabolome Sequencing in Muscle
2.5. Transcriptome Sequencing in Intestines
2.6. 16S rRNA Sequencing in Intestinal Bacteria
2.7. Statistical Analysis
3. Results
3.1. Common Carp Growth Performance
3.2. Antioxidant and Lipid Peroxidation Parameters in Different Tissues
3.3. Amino Acid and Fatty Acid Composition in Muscle
3.4. Metabolomics Analysis in Muscle
3.5. Transcriptomic Analysis in Intestines
3.6. Intestinal Microbiota Characteristics
3.7. Interactions between Intestinal Microbes and Lipid Metabolism
4. Discussion
4.1. The Effect of Pros on Growth Performance
4.2. The Effect of Pros on Antioxidant Capacity
4.3. The Effect of Pros on Muscle Nutrient Quality
4.4. The Effect of Pros on Lipid Metabolism in Intestines
4.5. The Effect of Pros on Intestinal Microbiota
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Growth Index | NC | 0.2 g/kg Pro | 0.4 g/kg Pro | 0.8 g/kg Pro |
---|---|---|---|---|
Initial body weight (g) | 42.5 ± 1.71 | 42.6 ± 1.63 | 42.6 ± 1.89 | 40.5 ± 2.01 |
Final body weight (g) | 89.9 ± 3.48 a | 86.5 ± 3.38 a | 101.5 ± 2.80 b | 103.5 ± 3.92 b |
Specific growth rate (%/d) | 1.07 ± 0.05 a | 1.01 ± 0.06 a | 1.24 ± 0.04 b | 1.35 ± 0.01 b |
Feed conversion ratio | 2.13 ± 0.02 a | 2.07 ± 0.02 a | 1.83 ± 0.04 b | 1.85 ± 0.03 b |
Survival ratio (%) | 96.7 | 96.7 | 100 | 96.7 |
Fatty Acids (% of Total Fatty Acid) | Groups | ||
---|---|---|---|
NC | 0.8 g/kg Pro | Sig. | |
C16:0 | 19.92 ± 0.41 | 22.41 ± 0.18 | ** |
C18:0 | 7.72 ± 0.3 | 9.49 ± 0.15 | ** |
C18:1n9c | 22.67 ± 2.63 | 31.03 ± 2.79 | ns |
C18:2n6c | 21.92 ± 2.05 | 29.5 ± 2.06 | ns |
C18:3n3 | 0.99 ± 0.04 | 1.72 ± 0.1 | ** |
C20:1 | 1.04 ± 0.04 | 1.32 ± 0.07 | * |
C20:2 | 0.94 ± 0.08 | 1.20 ± 0.02 | * |
C20:3n6 | 2.53 ± 0.11 | 2.97 ± 0.13 | ns |
C22:1n9 | 0.84 ± 0.02 | 1.10 ± 0.03 | ** |
C20:4n6 | 5.73 ± 0.35 | 7.81 ± 0.35 | * |
C22:6n3 | 3.24 ± 0.39 | 3.95 ± 0.11 | ns |
Total SFA | 27.64 ± 0.7 | 31.91 ± 0.32 | ** |
Total MUFA | 24.55 ± 2.65 | 33.44 ± 2.84 | ns |
Total PUFA | 35.35 ± 1.4 | 47.15 ± 1.81 | ** |
n-3 PUFA | 4.23 ± 0.41 | 5.67 ± 0.07 | * |
n-6 PUFA | 30.18 ± 1.74 | 40.28 ± 1.73 | * |
n-6/n-3 | 7.61 ± 1.3 | 7.09 ± 0.24 | ns |
PUFA/SFA | 1.28 ± 0.06 | 1.48 ± 0.08 | ns |
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Jia, R.; Hou, Y.; Feng, W.; Nomingerel, M.; Li, B.; Zhu, J. Multi-Omics Analysis to Understand the Effects of Dietary Proanthocyanidins on Antioxidant Capacity, Muscle Nutrients, Lipid Metabolism, and Intestinal Microbiota in Cyprinus carpio. Antioxidants 2023, 12, 2095. https://doi.org/10.3390/antiox12122095
Jia R, Hou Y, Feng W, Nomingerel M, Li B, Zhu J. Multi-Omics Analysis to Understand the Effects of Dietary Proanthocyanidins on Antioxidant Capacity, Muscle Nutrients, Lipid Metabolism, and Intestinal Microbiota in Cyprinus carpio. Antioxidants. 2023; 12(12):2095. https://doi.org/10.3390/antiox12122095
Chicago/Turabian StyleJia, Rui, Yiran Hou, Wenrong Feng, Munkhjargal Nomingerel, Bing Li, and Jian Zhu. 2023. "Multi-Omics Analysis to Understand the Effects of Dietary Proanthocyanidins on Antioxidant Capacity, Muscle Nutrients, Lipid Metabolism, and Intestinal Microbiota in Cyprinus carpio" Antioxidants 12, no. 12: 2095. https://doi.org/10.3390/antiox12122095