Entomotherapeutic Role of Periplaneta americana Extract in Alleviating Aluminum Oxide Nanoparticles-Induced Testicular Oxidative Impairment in Migratory Locusts (Locusta migratoria) as an Ecotoxicological Model
Abstract
:1. Introduction
2. Materials and Methods
2.1. Collection of Insects
2.2. Al2O3 NPs Characterization
2.3. Preparation of Al2O3 NPs Suspension and PAE Solution
2.4. Experimental Design
2.5. Dissection Procedures
2.6. X-ray Detection of Al in the Testes of L. migratoria
2.7. Biochemical Evaluations
2.7.1. Determination of Protein Content in Hemolymph and Total Hemocyte Count
2.7.2. Biochemical Assays in Testicular Tissues
2.7.3. Molecular Docking and Computational Studies
2.7.4. Assessment of HSP70 and HSP90 mRNA Expressions
2.8. Cells Viability Assessment by Flow Cytometry
2.9. Assessment of DNA Damage
2.10. Scanning Electron Microscope (SEM) Analysis of Testes
2.11. Histological and Ultrastructural Analyses of Testicular Tissues
2.12. Statistical Analysis
3. Results
3.1. Characterization of Al2O3 NPs
3.2. Survival and Mortality Analyses of L. migratoria Male after Injection with Al2O3 NPs and PAE
3.3. Evaluation of Al Accumulated in Testicular Tissues of Locusts
3.4. Impact of Al2O3 NPs and Combinatorial Treatment of Al2O3 NPs and PAE on the Physiological Properties of L. migratoria
3.5. Evaluation of DNA Impairment by Comet Assay
3.6. Assessment of Cell Viability by Flow Cytometric Analysis
3.7. Molecular Docking Analysis
3.8. SEM Analysis
3.9. Histological Analysis
3.10. Ultrastructural Analysis
4. Discussion
4.1. Size and Bioaccumulation of Al2O3 in the Testicular Tissues
4.2. Impact of Alumina and PAE on Hemocytes
4.3. Al-induced Oxidative Stress in the Locust Testes
4.4. Apoptotic Analysis and DNA Damage of Testicular Tissues
4.5. Histological and Ultrastructural Analyses of Testicular Tissues
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Control Group | Group Treated with Al2O3 NPs | Group Treated with PAE + Al2O3 NPs | ||||
---|---|---|---|---|---|---|
Element | Mass (%) | Atom (%) | Mass (%) | Atom (%) | Mass (%) | Atom (%) |
C | 55.47 ± 0.30 | 62.00 ± 0.33 | 43.97 ± 0.29 | 50.12 ± 0.33 | 44.86 ± 0.29 | 51.10 ± 0.33 |
N | 9.63 ± 0.56 | 9.23 ± 0.54 | 18.83 ± 0.66 | 18.40 ± 0.64 | 20.93 ± 0.66 | 20.45 ± 0.65 |
O | 33.45 ± 0.61 | 28.07 ± 0.52 | 36.26 ± 0.70 | 31.03 ± 0.60 | 32.11 ± 0.65 | 27.46 ± 0.56 |
Na | 0.46 ± 0.06 | 0.27 ± 0.03 | 0.13 ± 0.05 | 0.08 ± 0.03 | 0.46 ± 0.06 | 0.28 ± 0.03 |
P | 0.63 ± 0.04 | 0.27 ± 0.02 | 0.24 ± 0.04 | 0.11 ± 0.02 | 0.74 ± 0.05 | 0.33 ± 0.02 |
S | 0.36 ± 0.03 | 0.15 ± 0.01 | 0.33 ± 0.04 | 0.14 ± 0.02 | 0.83 ± 0.05 | 0.35 ± 0.02 |
Al | --- | --- | 0.25 ± 0.04 | 0.13 ± 0.02 | 0.07 ± 0.03 | 0.03 ± 0.01 |
Ligand | 1AR5 | 8CAT | ||
---|---|---|---|---|
Ebinding (kcal/mol) | EIntermol. (kcal/mol) | Ebinding (kcal/mol) | EIntermol. (kcal/mol) | |
MHI | −5.79 | −6.09 | −6.03 | −6.93 |
MTTD | −4.66 | −8.24 | 16.14 | 12.56 |
MTP | 171.76 | 168.78 | 470.36 | 467.37 |
PAE | −3.79 | −4.68 | −4.12 | −5.02 |
OAM | −3.73 | −8.56 | 20.11 | 15.34 |
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Arafat, E.A.; El-Sayed, D.S.; Hussein, H.K.; Flaven-Pouchon, J.; Moussian, B.; El-Samad, L.M.; El Wakil, A.; Hassan, M.A. Entomotherapeutic Role of Periplaneta americana Extract in Alleviating Aluminum Oxide Nanoparticles-Induced Testicular Oxidative Impairment in Migratory Locusts (Locusta migratoria) as an Ecotoxicological Model. Antioxidants 2023, 12, 653. https://doi.org/10.3390/antiox12030653
Arafat EA, El-Sayed DS, Hussein HK, Flaven-Pouchon J, Moussian B, El-Samad LM, El Wakil A, Hassan MA. Entomotherapeutic Role of Periplaneta americana Extract in Alleviating Aluminum Oxide Nanoparticles-Induced Testicular Oxidative Impairment in Migratory Locusts (Locusta migratoria) as an Ecotoxicological Model. Antioxidants. 2023; 12(3):653. https://doi.org/10.3390/antiox12030653
Chicago/Turabian StyleArafat, Esraa A., Doaa S. El-Sayed, Hussein K. Hussein, Justin Flaven-Pouchon, Bernard Moussian, Lamia M. El-Samad, Abeer El Wakil, and Mohamed A. Hassan. 2023. "Entomotherapeutic Role of Periplaneta americana Extract in Alleviating Aluminum Oxide Nanoparticles-Induced Testicular Oxidative Impairment in Migratory Locusts (Locusta migratoria) as an Ecotoxicological Model" Antioxidants 12, no. 3: 653. https://doi.org/10.3390/antiox12030653
APA StyleArafat, E. A., El-Sayed, D. S., Hussein, H. K., Flaven-Pouchon, J., Moussian, B., El-Samad, L. M., El Wakil, A., & Hassan, M. A. (2023). Entomotherapeutic Role of Periplaneta americana Extract in Alleviating Aluminum Oxide Nanoparticles-Induced Testicular Oxidative Impairment in Migratory Locusts (Locusta migratoria) as an Ecotoxicological Model. Antioxidants, 12(3), 653. https://doi.org/10.3390/antiox12030653