Chronic Vitamin E Deficiency Dysregulates Purine, Phospholipid, and Amino Acid Metabolism in Aging Zebrafish Skeletal Muscle
Abstract
:1. Introduction
2. Materials and Methods
2.1. Materials and Reagents
2.2. Zebrafish Husbandry and Dietary Conditions
2.3. Muscle Extraction
2.4. LC-MS/MS for Metabolomic Analysis
2.5. Quantitation and Statistical Analysis of α-Tocopherol in Tissues
2.6. Metabolomics Data Processing and Statistical Analyses
3. Results
3.1. Muscle Vitamin E Concentrations
3.2. Effect of Age on Muscle Metabolites
3.3. Effect at 12 Months of Vitamin E Deficiency on Muscle Metabolites
3.4. Effect of 18 Months of Vitamin E Deficiency of Muscle Metabolites
3.5. Effect of Prolonged Vitamin E Deficiency on Muscle Metabolites
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Henderson, T.D.; Choi, J.; Leonard, S.W.; Head, B.; Tanguay, R.L.; Barton, C.L.; Traber, M.G. Chronic Vitamin E Deficiency Dysregulates Purine, Phospholipid, and Amino Acid Metabolism in Aging Zebrafish Skeletal Muscle. Antioxidants 2023, 12, 1160. https://doi.org/10.3390/antiox12061160
Henderson TD, Choi J, Leonard SW, Head B, Tanguay RL, Barton CL, Traber MG. Chronic Vitamin E Deficiency Dysregulates Purine, Phospholipid, and Amino Acid Metabolism in Aging Zebrafish Skeletal Muscle. Antioxidants. 2023; 12(6):1160. https://doi.org/10.3390/antiox12061160
Chicago/Turabian StyleHenderson, Trent D., Jaewoo Choi, Scott W. Leonard, Brian Head, Robyn L. Tanguay, Carrie L. Barton, and Maret G. Traber. 2023. "Chronic Vitamin E Deficiency Dysregulates Purine, Phospholipid, and Amino Acid Metabolism in Aging Zebrafish Skeletal Muscle" Antioxidants 12, no. 6: 1160. https://doi.org/10.3390/antiox12061160