Spermidine Improves Freezing Tolerance by Regulating H2O2 in Brassica napus L.
Abstract
:1. Introduction
2. Materials and Methods
2.1. Plant Materials
2.2. Freezing Treatment
2.3. Determination of H2O2 Concentration
2.4. RNA-Seq
2.5. Quantitative Real-Time PCR Analysis
2.6. Statistics and Analysis
3. Results
3.1. Two Concentrations of Exogenous Spd Improve the Freezing Tolerance of Seedlings
3.2. Transcriptome Sequencing Analysis of the Effect of Spd on Rapeseed Seedlings under Freezing Treatment
3.3. Gene Ontology Functional and Kyoto Encyclopedia of Genes and Genomes Pathway Enrichment Analysis
3.4. qRT-PCR Validation of DEGs
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
Appendix A
Primer Name | ZS11 Gene ID | Primer Sequence (5′→3′) |
---|---|---|
Darmor Gene ID | ||
Actin-F | - | GGTTGGGATGGACCAGAAGG |
Actin-R | BnaC05g34300D | TCAGGAGCAATACGGAGC |
DREB1C_qF | BnaA03G0486800ZS | CGGCAAATCAGCTTGTCTCAA |
DREB1C_qR | - | GCAGCCGCCTTCTGAAATCT |
AZF2_qF | BnaA05G0364000ZS | CAAGACGCAACCGCAAGAAT |
AZF2_qR | BnaA05g20490D | GTTGTTGAATCGTCGTCGGC |
COR27_qF | BnaA06G0444700ZS | GCCAAAACGGAAGCTCCAAG |
COR27_qR | BnaA06g37010D | AGTGGAACAACCTATACGCCA |
SPE2_qF | BnaA08G0133500ZS | CCGTATCTAGCGACTGAGCC |
SPE2_qR | BnaA08g10990D | CCACACGACTACCCTTGTCA |
CML4_qF | BnaA09G0541500ZS | ACCGACGACAAGATCACACC |
CML4_qR | BnaA09g37780D | GCCGTCTCCGTTCTTGTCA |
ERF019_qF | BnaC05G0198700ZS | TAGTTTCCAAGACCTCGCCG |
ERF019_qR | BnaC05g18050D | CGTGATCGTGACCGTCCAAA |
NAC055_qF | BnaC05G0447000ZS | TACGTTACGTTAACCGGGTCG |
NAC055_qR | BnaC05g38150D | TACTTCCTGTCCCTTGGGCT |
DREB1B_qF | BnaC08G0165900ZS | GAGTCTCCGGTAGGTGGTGA |
DREB1B_qR | - | GTGACGTGTCTCCCGAAACT |
JAZ1_qF | BnaC08G0251600ZS | CATCGCTCCTACCTCGAACC |
JAZ1_qR | BnaC08g18640D | TGAGATAGATTACCGCATGCCA |
AOS-qF | BnaA02G0279100ZS | TTCTCTTCACGCTCGGCTTCAC |
AOS-qR | BnaA02g23180D | ACGGCTAGATCAGGAGGAGTCTC |
AOC2-qF | BnaA06G0393700ZS | TTACAGCTTCTACTTCGGCGACTAC |
AOC2-qR | BnaA06g33410D | GGTGATGGCGAGGAACGAGTC |
CuAOs_qF | BnaA03G0591500ZS | CTTGATTCGGGTCGGGTCGTTTC |
CuAOs_qR | - | GCGATACCTGAGAAGCCACGATG |
Appendix B
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Li, S.; Liu, Y.; Kang, Y.; Liu, W.; Wang, W.; Wang, Z.; Xia, X.; Chen, X.; Wang, C.; He, X. Spermidine Improves Freezing Tolerance by Regulating H2O2 in Brassica napus L. Antioxidants 2024, 13, 1032. https://doi.org/10.3390/antiox13091032
Li S, Liu Y, Kang Y, Liu W, Wang W, Wang Z, Xia X, Chen X, Wang C, He X. Spermidine Improves Freezing Tolerance by Regulating H2O2 in Brassica napus L. Antioxidants. 2024; 13(9):1032. https://doi.org/10.3390/antiox13091032
Chicago/Turabian StyleLi, Shun, Yan Liu, Yu Kang, Wei Liu, Weiping Wang, Zhonghua Wang, Xiaoyan Xia, Xiaoyu Chen, Chen Wang, and Xin He. 2024. "Spermidine Improves Freezing Tolerance by Regulating H2O2 in Brassica napus L." Antioxidants 13, no. 9: 1032. https://doi.org/10.3390/antiox13091032