Sodium Houttuybonate Promotes the Browning of White Adipose Tissue by Inhibiting Ferroptosis via the AMPK-NRF2-HO1 Pathway
Abstract
:1. Introduction
2. Materials and Methods
2.1. Mice and Treatments
2.2. Glucose Tolerance Tests (GTTs)
2.3. Metabolic and Biochemical Analyses
2.4. Histological and Immunohistochemical Analyses
2.5. Primary SVF Isolation, Maturation, and Treatment
2.6. Quantitative PCR (qPCR) Analysis
2.7. Western Blotting
2.8. Data Statistics
2.9. Data and Resource Availability
3. Results
3.1. SH Promotes iWAT Browning and Prevents Diet-Induced Obesity
3.2. Effects of SH on Adipose Tissue under Cold Stimulation
3.3. Effects of SH on Adipose Tissue at Thermoneutrality
3.4. SH Directly Promotes the Browning of Adipocytes In Vitro
3.5. SH Enhances Adipocyte Browning by Inhibiting Ferroptosis
3.6. SH Promotes iWAT Browning via the AMPK-NRF2-HO1 Axis
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Liu, W.; Zou, H.; You, D.; Zhang, H.; Xu, L. Sodium Houttuybonate Promotes the Browning of White Adipose Tissue by Inhibiting Ferroptosis via the AMPK-NRF2-HO1 Pathway. Antioxidants 2024, 13, 1057. https://doi.org/10.3390/antiox13091057
Liu W, Zou H, You D, Zhang H, Xu L. Sodium Houttuybonate Promotes the Browning of White Adipose Tissue by Inhibiting Ferroptosis via the AMPK-NRF2-HO1 Pathway. Antioxidants. 2024; 13(9):1057. https://doi.org/10.3390/antiox13091057
Chicago/Turabian StyleLiu, Wenhui, Huren Zou, Danming You, Huijie Zhang, and Lingling Xu. 2024. "Sodium Houttuybonate Promotes the Browning of White Adipose Tissue by Inhibiting Ferroptosis via the AMPK-NRF2-HO1 Pathway" Antioxidants 13, no. 9: 1057. https://doi.org/10.3390/antiox13091057