MiR155-5p Inhibits Cell Migration and Oxidative Stress in Vascular Smooth Muscle Cells of Spontaneously Hypertensive Rats
Abstract
:1. Introduction
2. Materials and Methods
2.1. Animals
2.2. Culture of Primary VSMCs
2.3. Boyden Chamber Assay
2.4. Wound-Healing Assay
2.5. Transfection of miR155-5p Mimic and Inhibitor
2.6. MiR155-5p Overexpression and Ang II Treatment in VSMCs
2.7. Western Blot Analysis
2.8. Measurement of miR155-5p Level with qPCR
2.9. Measurement of ACE mRNA Level with qPCR
2.10. DHE Fluorescence Staining
2.11. Measurement of NAD(P)H Oxidase Activity
2.12. Statistics and Data Analysis
3. Results
3.1. Effects of miR155-5p Mimic and Inhibitor on VSMC Migration
3.2. Effects of miR155-5p Mimic and Inhibitor on ACE Expression
3.3. Effects of miR155-5p Mimic on Oxidative Stress
3.4. Effects of miR155-5p Mimic on IL-1β and TNF-α Levels
3.5. Effects of miR155-5p Overexpression on Ang II-Induced VSMC Migration
3.6. Effects of miR155-5p Overexpression on Ang II-Induced Oxidative Stress and Inflammation
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
Data Availability
References
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Wu, N.; Ye, C.; Zheng, F.; Wan, G.-W.; Wu, L.-L.; Chen, Q.; Li, Y.-H.; Kang, Y.-M.; Zhu, G.-Q. MiR155-5p Inhibits Cell Migration and Oxidative Stress in Vascular Smooth Muscle Cells of Spontaneously Hypertensive Rats. Antioxidants 2020, 9, 204. https://doi.org/10.3390/antiox9030204
Wu N, Ye C, Zheng F, Wan G-W, Wu L-L, Chen Q, Li Y-H, Kang Y-M, Zhu G-Q. MiR155-5p Inhibits Cell Migration and Oxidative Stress in Vascular Smooth Muscle Cells of Spontaneously Hypertensive Rats. Antioxidants. 2020; 9(3):204. https://doi.org/10.3390/antiox9030204
Chicago/Turabian StyleWu, Nan, Chao Ye, Fen Zheng, Guo-Wei Wan, Lu-Lu Wu, Qi Chen, Yue-Hua Li, Yu-Ming Kang, and Guo-Qing Zhu. 2020. "MiR155-5p Inhibits Cell Migration and Oxidative Stress in Vascular Smooth Muscle Cells of Spontaneously Hypertensive Rats" Antioxidants 9, no. 3: 204. https://doi.org/10.3390/antiox9030204