Study on the Surface Interactions of Co(II) with Phospholipids from the Marine Environment
Round 1
Reviewer 1 Report
Dear Authors,
The submitted paper entitled Study on the surface interactions of Co(II) with phospholipids from the marine environment shows a three methods to determine surface interactions of Co(II) with various type of lipids. The study is very wide and profound. However, some minor revision and text editing is required.
Figure 1 is not informative. Authors shell consider to revise the picture in terms to have more information of extraction procedure. They might consider a flowchart.
In various parts of text square millimeter is written as mm2. Please use mm2. E.g.: page 4, line 133
On page 5 line 182 it is written: "... by washing the samples with water ...". The authors should write what type of water or solution is used in this part.
The authors should explain why PC, PG and PE lipids were used in the study.
The authors have joint the Results and Discussion. It is expected that comment of different parameters provided by each method will be compared and explained.
In figure 4 the SW voltamograms of Co(II) due to a title should be main graph.
Some small typos to be corrected:
page 2, line 63: the space before semicolon shell be removed
page 10, line 350 "graphic image was recorded registered" - one verb should be used only.
page 12, line 376 Word "Although" does not fit in the text.
Author Response
We want to thank to all reviewers for their methodical and very useful comments, as well as for supportive judgement and helpful advices.
We appreciate very much positive comments of Reviewers about understanding and quality of our results. In revised version, we have taken into account comments of all Reviewers, and improved the text according to suggestions.
We thank the referee 1 for positively evaluating our manuscript, taking an in-depth look on our manuscript, and providing constructive comments. We believe we have now answered the questions that were raised and consequently improved the manuscript.
Author Response File: 
Author Response.pdf
Reviewer 2 Report
The manuscript of Bačinić et al. regarding the interaction of Co(II) with phospholipids and phenanthroline is well written. Methods and results provide enough details to understand and support the statements in conclusions. I have only a few minor issues that could improve the work.
1) Title: Why is phenanthroline not included in the title? Probably it should as “The interaction of Co(II) with natural and standard phospholipids (…) are registered only in the presence of the additional chelating ligand 1,10-Phenathroline (Phen).”
2) The identification of the type of phospholipids is based on MS and then compared with literature data. Is it enough? Do you have any structures and theoretical masses that could be compared with experimental data?
3) Fig. 2. The accuracy of mass values could be reduced to a maximum of one or two decimal places.
Author Response
We want to thank to all reviewers for their methodical and very useful comments, as well as for supportive judgement and helpful advices.
We appreciate very much positive comments of Reviewers about understanding and quality of our results. In revised version, we have taken into account comments of all Reviewers, and improved the text according to suggestions.
We thank Referee 2 for positively evaluating our manuscript and providing constructive comments. We believe we have answered minor comments and subsequently improved the manuscript.
Author Response File: Author Response.pdf
