Coagulant Plus Bacillus nitratireducens Fermentation Broth Technique Provides a Rapid Algicidal Effect of Toxic Red Tide Dinoflagellate

Round 1

Reviewer 1 Report

Authors demonstrated the application of  Polyaluminum chloride (PAC) to trat the red tide bloom of Gymnodinium catenatum  at 90mg/L, the removal rate reached 100%. 

1. In introduction section add,  the impact of red tide bloom 

2. Figure 1 representation is very poor,  Should be replaced with better version. 

3. The details of microscope used for this study and preparation of sample for analysis?

4. The details of UV spectroscopy used for this study and preparation of sample for analysis, and concentration of sample etc., ?

 

 

Author Response

Response to Editor and Reviewers’ Comments

                   25 March 2021

 

Manuscript ID: jmse-1160869
Type of manuscript: Article
Title: Combined algicidal effect of Bacillus nitratireducens fermentation broth and PAC coagulantion to toxic dinoflagellate Gymnodinium catenatum

Authors: Balaji P. Barathan, Hong Lin, Yu P. Su^*, Wan N. Zheng, Ying Wang, Hong Yang

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Dear Editors and Reviewers:

 

We would like to thank you for your time and effort in reviewing our manuscript, and for your suggestion on improving the quality of our manuscript. We have incorporated almost all suggestions from you.

The following is a point-by-point response to the reviewer’s comments. All revised items were marked in red in the text, tables, and figures. The minor changes have been marked in red color in the new version; we have done our best to improve the quality of the paper.

 

Response to Editor’s comments

Comment: Please revise the manuscript according to the reviewers' comments and upload the revised file within 2 days. Use the version of your manuscript found at the above link for your revisions, as the editorial office may have made formatting changes to your original submission. Any revisions should be clearly highlighted, for example using the "Track Changes" function in Microsoft Word, so that they are easily visible to the editors and reviewers. Please provide a short cover letter detailing any changes, for the benefit of the editors and reviewers.

Response: The authors carefully revised the manuscript based on the reviewer’s comments. The revised one highlighted in ‘red’ color. According to the reviewers’ comments, point-by-point the details of the revisions have been made in the manuscript

Response to Reviewer 1:

 

Comment: In introduction section add, the impact of red tide bloom

 

Response:  In introduction, we have included the impact of red tide bloom

 

Comment: Figure 1 representation is very poor, Should be replaced with better version.

Response: It has changed according to the reviewer’s comments

 

Comment: The details of microscope used for this study and preparation of sample for analysis?

Response: Overall structure and sections have been modified based on the reviewer’s comments

 

 

Comment: The details of UV spectroscopy used for this study and preparation of sample for analysis, and concentration of sample etc., ?

Response:  UV spectroscopy used for this study and preparation of the sample for analysis have written extensively based on the reviewer’s comments.

 

 

Thanks again for your suggestion.

 

 

Prof. Yuping Su

College of Environmental Science and Engineering,

Fujian Normal University

Fuzhou 350007, PR China.

Email: [email protected]

Reviewer 2 Report

Dear Editor and Authors,

The manuscript entitled “Combined algicidal effect of Bacillus nitratireducens fermentation broth and PAC coagulation to toxic dinoflagellate Gymnodinium catenatum” provides a basis for improving the control of the marine red tide caused by the dinoflagellate G. catenatum, using environmentally friendly methods.

Only few points must be addressed.

 

Title: Replace “coangulation” by “coagulation”.

Also in the Title, the authors should not use abbreviations.

The species identifier should be indicated the first time it is mentioned in the manuscript (Gymnodinium catenatum H.W. Graham).

Keywords: Replace G. catenatum by Gymnodinium catenatum.

The authors need to uniformize: a) the measuring units (mL instead of ml); b) the space between number and units (e.g., 10 g/L instead of 10g/L); c) the abbreviation used for the bacterial fermentation broth (is it ba3, Ba3?).

Section 2.4.2: 180r/min is 180 rpm?

Figures 4, 6, and 9 have low definition. In figure captions, avoid using “4(B)”. Instead, place only the letter before or after each description.

Add a “Conclusions” section and improve the conclusions drawn. How can your research effectively improve the control of marine red tide? Provide some future perspectives, highlighting the novelty of your work, and the interest of your results, opening doors for the effective application.

Comments for author File: Comments.pdf

Author Response

Response to Editor and Reviewers’ Comments

                             25 March, 2021

 

Manuscript ID: jmse-1160869
Type of manuscript: Article
Title: Combined algicidal effect of Bacillus nitratireducens fermentation broth and PAC coagulantion to toxic dinoflagellate Gymnodinium catenatum

Authors: Balaji P. Barathan, Hong Lin, Yu P. Su^*, Wan N. Zheng, Ying Wang, Hong Yang

-------------------------------------------------------------------------------------------------------

 

Dear Editors and Reviewers:

 

We would like to thank you for your time and effort in reviewing our manuscript, and for your suggestion on improving the quality of our manuscript. We have incorporated almost all suggestions from you.

 

The following is a point-by-point response to the reviewer’s comments. All revised items were marked in red in the text, tables, and figures. The minor changes have been marked in red color in the new version; we have done our best to improve the quality of the paper.

 

Response to Editor’s comments

 

Comment: Please revise the manuscript according to the reviewers' comments and upload the revised file within 2 days. Use the version of your manuscript found at the above link for your revisions, as the editorial office may have made formatting changes to your original submission. Any revisions should be clearly highlighted, for example using the "Track Changes" function in Microsoft Word, so that they are easily visible to the editors and reviewers. Please provide a short cover letter detailing any changes, for the benefit of the editors and reviewers.

Response: The authors carefully revised the manuscript based on the reviewer’s comments. The revised one highlighted in ‘red’ color. According to the reviewers’ comments, point-by-point the details of the revisions have been made in the manuscript

Response to reviewer 2

Comment: Title: Replace “coangulation” by “coagulation”

Response: It has changed according to the reviewer’s comments

 

 

Comment: Also in the Title, the authors should not use abbreviations.

Response: It has changed according to the reviewer’s comments

 

Comment:The species identifier should be indicated the first time it is mentioned in the manuscript (Gymnodinium catenatum H.W. Graham)

Response: It has changed according to the reviewer’s comments

 

Comment:Keywords: Replace G. catenatum by Gymnodinium catenatum.

Response: It has changed according to the reviewer’s comments

 

Comment:The authors need to uniformize: a) the measuring units (mL instead of ml); b) the space between number and units (e.g., 10 g/L instead of 10g/L); c) the abbreviation used for the bacterial fermentation broth (is it ba3, Ba3?).

Response: These comments have reviewed throughout the manuscript

 

Comment: Section 2.4.2: 180r/min is 180 rpm?

Response: It has changed according to the reviewer’s comments

 

Comment: Figures 4, 6, and 9 have low definition. In figure captions, avoid using “4(B)”. Instead, place only the letter before or after each description.

Response: It has changed according to the reviewer’s comments

 

Comment: Add a “Conclusions” section and improve the conclusions drawn. How can your research effectively improve the control of marine red tide? Provide some future perspectives, highlighting the novelty of your work, and the interest of your results, opening doors for the effective application.

Response: In conclusion, we have included the future perspectives of the study

 

 

Thanks again for your suggestion.

 

 

Prof. Yuping Su

College of Environmental Science and Engineering,

Fujian Normal University

Fuzhou 350007, PR China.

Email:[email protected]