Homeostatic Functions of Tecrem, a CD46-Like Regulatory Protein of Complement Activation, on Epithelial Cells in Carp Fish
Abstract
:1. Introduction
2. Materials and Methods
2.1. Materials
2.2. Detection of Tecrem Protein by ELISA and Immunoprecipitation
2.3. Expression of Recombinant SCR1-2 and SCR3-4 Domains of cTecrem
2.4. Preparation of Rabbit Polyclonal Anti-cTecrem Domains
2.5. Flow Cytometric Analysis of Tecrem Expression on KF-1 Cells
2.6. Western Blotting Analysis of Tecrem Expression on KF-1 and CFS Cells
2.7. Adhesion Assays of KF-1 Cells
2.8. Wound Healing Assay
2.9. Immunohistochemistry Assay
3. Results
3.1. Preparation of Recombinant SCR 1-2 and SCR 3-4 and Its Polyclonal Antibodies
3.2. Activation of cTecrem Results in An Increase in the Adhesion of KF-1 Cells on to the Cell Culture Plate
3.3. Tecrem Activation Promotes Epithelial Cell Growth as well as Wound Healing
3.4. Enhancement of Tight Junction Protein Expression by cTecrem Stimulation in CFS Cells
3.5. Morphological Changes in the cTecrem-Activated KF-1 and CFS Cell Lines
4. Discussion
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Conflicts of Interest
References
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Name | Nucleotide Sequence 1 | Amino Acids Encoded | Strand |
---|---|---|---|
SCR1-2F | gcctcgagGCGGAATGTACACAGCCTTC | AECTQP | Sense |
SCR1-2R | cgctgcagttaCGATTCACAGAGAGGATCTC | DPLCES | Antisense |
SCR3-4F | gcctcgagGTAAAATGTTCAGCACCTCCAGC | VKCSAPP | Sense |
SCR3-4R | cgctgcagttaGACAATGCATTGTGGTGGTTCAG | EPPQCIV | Antisense |
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Prakash, H.; Motobe, S.; Nagasawa, T.; Somamoto, T.; Nakao, M. Homeostatic Functions of Tecrem, a CD46-Like Regulatory Protein of Complement Activation, on Epithelial Cells in Carp Fish. J. Mar. Sci. Eng. 2021, 9, 687. https://doi.org/10.3390/jmse9070687
Prakash H, Motobe S, Nagasawa T, Somamoto T, Nakao M. Homeostatic Functions of Tecrem, a CD46-Like Regulatory Protein of Complement Activation, on Epithelial Cells in Carp Fish. Journal of Marine Science and Engineering. 2021; 9(7):687. https://doi.org/10.3390/jmse9070687
Chicago/Turabian StylePrakash, Harsha, Shiori Motobe, Takahiro Nagasawa, Tomonori Somamoto, and Miki Nakao. 2021. "Homeostatic Functions of Tecrem, a CD46-Like Regulatory Protein of Complement Activation, on Epithelial Cells in Carp Fish" Journal of Marine Science and Engineering 9, no. 7: 687. https://doi.org/10.3390/jmse9070687