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10.3390/nano10010050
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Article

Accelerated Osteogenic Differentiation of MC3T3-E1 Cells by Lactoferrin-Conjugated Nanodiamonds through Enhanced Anti-Oxidant and Anti-Inflammatory Effects

by
Sung Eun Kim
1,†,
Somang Choi
1,2,†,
Jae-Young Hong
3,†,
Kyu-Sik Shim
2,
Tae-Hoon Kim
4,
Kyeongsoon Park
5 and
Suk-Ha Lee
4,*
1
Department of Orthopedic Surgery and Rare Diseases Institute, Korea University Guro Hospital, #148, Gurodong-ro, Guro-gu, Seoul 08308, Korea
2
Department of Biomedical Science, College of Medicine, Korea University, Anam-dong, Seongbuk-gu, Seoul 02841, Korea
3
Department of Orthopedic Surgery, Korea University Ansan Hospital, #123, Jeokgeum-ro, Danwon-gu, Ansan-si, Gyeonggi-do 15355, Korea
4
Department of Orthopedic Surgery, Konkuk University Medical Center, Konkuk University School of Medicine, #120-1, Neungdong-ro, Hwayang-dong, Gwangjin-gu, Seoul 05030, Korea
5
Department of Systems Biotechnology, College of Biotechnology and Natural Resources, Chung-Ang University, 4726 Seodong-daero, Daedeok-myeon, Anseong-si, Gyeonggi-do 17546, Korea
*
Author to whom correspondence should be addressed.
†
These authore contributed equally to this work.
Nanomaterials 2020, 10(1), 50; https://doi.org/10.3390/nano10010050
Submission received: 2 December 2019 / Revised: 17 December 2019 / Accepted: 18 December 2019 / Published: 24 December 2019
(This article belongs to the Special Issue New Avenues of Research for Nanoparticle Drug Delivery Systems)
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Abstract

The purpose of this study was to investigate the effects of lactoferrin (LF)-conjugated nanodiamonds (NDs) in vitro on both anti-oxidant and anti-inflammation activity as well as osteogenic promotion. The application of LF-NDs resulted in sustained release of LF for up to 7 days. In vitro anti-oxidant analyses performed using Dichlorofluorescin diacetate (DCF-DA) assay and cell proliferation studies showed that LF (50 μg)-NDs effectively scavenged the reactive oxygen species (ROS) in MC3T3-E1 cells (osteoblast-like cells) after H2O2 treatment and increased proliferation of cells after H2O2 treatment. Treatment of lipopolysaccharide (LPS)-induced MC3T3-E1 cells with LF-NDs suppressed levels of pro-inflammatory cytokines, including interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α). In addition, LF-NDs were associated with outstanding enhancement of osteogenic activity of MC3T3-E1 cells due to increased alkaline phosphatase (ALP) and calcium deposition. Our findings suggest that LF-NDs are an important substrate for alleviating ROS effects and inflammation, as well as promoting osteogenic differentiation of cells.
Keywords: nanodiamonds; lactoferrin; anti-oxidant; anti-inflammatory; osteogenic differentiation nanodiamonds; lactoferrin; anti-oxidant; anti-inflammatory; osteogenic differentiation
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MDPI and ACS Style

Kim, S.E.; Choi, S.; Hong, J.-Y.; Shim, K.-S.; Kim, T.-H.; Park, K.; Lee, S.-H. Accelerated Osteogenic Differentiation of MC3T3-E1 Cells by Lactoferrin-Conjugated Nanodiamonds through Enhanced Anti-Oxidant and Anti-Inflammatory Effects. Nanomaterials 2020, 10, 50. https://doi.org/10.3390/nano10010050

AMA Style

Kim SE, Choi S, Hong J-Y, Shim K-S, Kim T-H, Park K, Lee S-H. Accelerated Osteogenic Differentiation of MC3T3-E1 Cells by Lactoferrin-Conjugated Nanodiamonds through Enhanced Anti-Oxidant and Anti-Inflammatory Effects. Nanomaterials. 2020; 10(1):50. https://doi.org/10.3390/nano10010050

Chicago/Turabian Style

Kim, Sung Eun, Somang Choi, Jae-Young Hong, Kyu-Sik Shim, Tae-Hoon Kim, Kyeongsoon Park, and Suk-Ha Lee. 2020. "Accelerated Osteogenic Differentiation of MC3T3-E1 Cells by Lactoferrin-Conjugated Nanodiamonds through Enhanced Anti-Oxidant and Anti-Inflammatory Effects" Nanomaterials 10, no. 1: 50. https://doi.org/10.3390/nano10010050

APA Style

Kim, S. E., Choi, S., Hong, J.-Y., Shim, K.-S., Kim, T.-H., Park, K., & Lee, S.-H. (2020). Accelerated Osteogenic Differentiation of MC3T3-E1 Cells by Lactoferrin-Conjugated Nanodiamonds through Enhanced Anti-Oxidant and Anti-Inflammatory Effects. Nanomaterials, 10(1), 50. https://doi.org/10.3390/nano10010050

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