Combination Design of Time-Dependent Magnetic Field and Magnetic Nanocomposites to Guide Cell Behavior
Abstract
:1. Introduction
2. Materials and Methods
2.1. Design and Manufacturing of PCL/Fe3O4 Nanocomposite Substrates
2.2. Small Punch Test
2.3. Scanning Electron Microscopy
2.4. Cell Culture
2.4.1. Magnetic Stimulation
2.4.2. Cell Metabolic Activity
2.4.3. Alkaline Phosphatase Activity
2.4.4. Confocal Laser Scanning Microscopy
2.4.5. Immunoblot Analysis
2.5. Statistical Analysis
3. Results
3.1. Small Punch Test
3.2. Scanning Electron Microscopy
3.3. Cell Metabolic Activity
3.4. Alkaline Phosphatase Activity
3.5. Confocal Laser Scanning Microscopy
3.6. Immunoblot Analysis
4. Discussion
5. Conclusions
- The possibility to develop PCL/Fe3O4 (80/20 w/w) substrates with improved mechanical properties (higher strength than PCL without negatively affecting the work to failure) was demonstrated by the small punch test.
- The combination of a time-dependent magnetic field with PCL/Fe3O4 nanocomposites (PCL/Fe3O4 Mag) impacted the behavior of hMSCs, especially resulting in a prolonged cell differentiation.
- The effect of a time-dependent magnetic field in increasing ERK phosphorylation levels and, hence, in the activation of the MAPK pathway, was reported, also corroborating previous findings on the combination of a magnetic field and magnetic nanocomposite structures.
- The role of the material–magnetic field combination was revealed, as the highest ERK phosphorylation levels were found in the case of PCL/Fe3O4 Mag.
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Materials | Peak Load (N) | Work to Failure (mJ) |
---|---|---|
PCL | 28.1 ± 2.1 | 31.4 ± 4.2 |
PCL/Fe3O4 (80/20 w/w) | 34.4 ± 2.6 | 30.1 ± 5.3 |
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Russo, T.; Peluso, V.; Gloria, A.; Oliviero, O.; Rinaldi, L.; Improta, G.; De Santis, R.; D’Antò, V. Combination Design of Time-Dependent Magnetic Field and Magnetic Nanocomposites to Guide Cell Behavior. Nanomaterials 2020, 10, 577. https://doi.org/10.3390/nano10030577
Russo T, Peluso V, Gloria A, Oliviero O, Rinaldi L, Improta G, De Santis R, D’Antò V. Combination Design of Time-Dependent Magnetic Field and Magnetic Nanocomposites to Guide Cell Behavior. Nanomaterials. 2020; 10(3):577. https://doi.org/10.3390/nano10030577
Chicago/Turabian StyleRusso, Teresa, Valentina Peluso, Antonio Gloria, Olimpia Oliviero, Laura Rinaldi, Giovanni Improta, Roberto De Santis, and Vincenzo D’Antò. 2020. "Combination Design of Time-Dependent Magnetic Field and Magnetic Nanocomposites to Guide Cell Behavior" Nanomaterials 10, no. 3: 577. https://doi.org/10.3390/nano10030577