Toxicity to RAW264.7 Macrophages of Silica Nanoparticles and the E551 Food Additive, in Combination with Genotoxic Agents
Abstract
:1. Introduction
2. Materials and Methods
2.1. Chemicals and Reagents
2.2. Cell Culture and Exposure
2.3. Cytotoxicity Assays
2.4. Genotoxicity Assays
2.5. RT-qPCR
2.6. Statistical Analysis
3. Results
3.1. Physico-Chemical Characterization of SiO2 Particles
3.2. Acute Cytotoxicity and Genotoxicity of SiO2 Particles
3.3. Cytotoxicity and Genotoxicity of LS30, Fumed Silica or E551 after Repeated Exposure
3.4. Cytotoxicity and Genotoxicity after Co-Exposure to SiO2 and B[a]P or MMS
4. Discussion
5. Conclusions
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Dussert, F.; Arthaud, P.-A.; Arnal, M.-E.; Dalzon, B.; Torres, A.; Douki, T.; Herlin, N.; Rabilloud, T.; Carriere, M. Toxicity to RAW264.7 Macrophages of Silica Nanoparticles and the E551 Food Additive, in Combination with Genotoxic Agents. Nanomaterials 2020, 10, 1418. https://doi.org/10.3390/nano10071418
Dussert F, Arthaud P-A, Arnal M-E, Dalzon B, Torres A, Douki T, Herlin N, Rabilloud T, Carriere M. Toxicity to RAW264.7 Macrophages of Silica Nanoparticles and the E551 Food Additive, in Combination with Genotoxic Agents. Nanomaterials. 2020; 10(7):1418. https://doi.org/10.3390/nano10071418
Chicago/Turabian StyleDussert, Fanny, Pierre-Adrien Arthaud, Marie-Edith Arnal, Bastien Dalzon, Anaëlle Torres, Thierry Douki, Nathalie Herlin, Thierry Rabilloud, and Marie Carriere. 2020. "Toxicity to RAW264.7 Macrophages of Silica Nanoparticles and the E551 Food Additive, in Combination with Genotoxic Agents" Nanomaterials 10, no. 7: 1418. https://doi.org/10.3390/nano10071418