Oleuropein-Laded Ufasomes Improve the Nutraceutical Efficacy
Abstract
:1. Introduction
2. Materials and Methods
2.1. Materials
2.2. Preparation of Samples
2.3. Physico-Chemical and Technological Characterization of Carriers
2.4. Transmission Electron Microscopy (TEM)
2.5. Entrapment Efficacy of Samples
2.6. Oleuropein Release Profiles
2.7. HPLC Analysis
2.8. Cell Cultures and In Vitro Cytotoxic and Antioxidant Evaluation
2.9. Confocal Laser Scanning Microscopy (CLSM)
2.10. Statistical Analysis
3. Results
3.1. Physico-Chemical and Technological Characterization of Vesicular Carriers
3.2. In Vitro Cytotoxic Evaluation
3.3. Ufasomes-Cell Interaction
3.4. Oleuropein-Loaded Ufasomes Preparation and Physico-Chemical Characterization
3.5. Evaluation of Oleuropein Release Profile
3.6. Antioxidant Activity Evaluation: MTT and LDH Assay
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Formulation | Oleuropein (µM) | Lipid Composition (Molar Ratio) | ||
---|---|---|---|---|
Oleic Acid | Linoleic Acid | PL90G® | ||
A | - | 1 | 1 | 0.5 |
A10 | 10 | 1 | 1 | 0.5 |
A100 | 100 | 1 | 1 | 0.5 |
B | - | 1 | 1 | - |
B10 | 10 | 1 | 1 | - |
B100 | 100 | 1 | 1 | - |
Sample | Lipid Composition (Molar Ratio) | Mean Size (nm) | Polidispersity Index | Zeta Potential (mV) | ||
---|---|---|---|---|---|---|
Oleic Acid | Linoleic Acid | PL90G | ||||
A | 1 | 1 | 0.5 | 185 ± 2 | 0.18 ± 0.01 | −43 ± 1 |
B | 1 | 1 | - | 280 ± 5 | 0.21 ± 0.02 | −41 ± 1 |
Sample | Mean Size (nm) | Polidispersity Index | Zeta Potential (mV) | EE (%) |
---|---|---|---|---|
A | 185 ± 2 | 0.18 ± 0.01 | −43 ± 1 | - |
A10 | 184 ± 1 | 0.21 ± 0.01 | −39 ± 2 | 85 ± 1 |
A100 | 199 ± 1 | 0.25 ± 0.03 | −42 ± 1 | 89 ± 2 |
B | 280 ± 5 | 0.21 ± 0.02 | −41 ± 1 | - |
B10 | 350 ± 6 | 0.84 ± 0.06 | −38 ± 5 | 45 ± 1 |
B100 | - | - | - | - |
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Cristiano, M.C.; Froiio, F.; Mancuso, A.; Cosco, D.; Dini, L.; Di Marzio, L.; Fresta, M.; Paolino, D. Oleuropein-Laded Ufasomes Improve the Nutraceutical Efficacy. Nanomaterials 2021, 11, 105. https://doi.org/10.3390/nano11010105
Cristiano MC, Froiio F, Mancuso A, Cosco D, Dini L, Di Marzio L, Fresta M, Paolino D. Oleuropein-Laded Ufasomes Improve the Nutraceutical Efficacy. Nanomaterials. 2021; 11(1):105. https://doi.org/10.3390/nano11010105
Chicago/Turabian StyleCristiano, Maria Chiara, Francesca Froiio, Antonia Mancuso, Donato Cosco, Luciana Dini, Luisa Di Marzio, Massimo Fresta, and Donatella Paolino. 2021. "Oleuropein-Laded Ufasomes Improve the Nutraceutical Efficacy" Nanomaterials 11, no. 1: 105. https://doi.org/10.3390/nano11010105