Feasibility of Application of the Newly Developed Nano-Biomaterial, β-TCP/PDLLA, in Maxillofacial Reconstructive Surgery: A Pilot Rat Study
Abstract
:1. Introduction
2. Materials and Methods
2.1. Materials
2.2. Animals
2.3. Anesthetic Agents
2.4. Surgical Procedures
2.5. Micro-Computed Tomography
2.6. Bone Mineral Density and the Bone Volume to Total Volume Ratio
2.7. Histochemical Staining
2.8. Optical Density Assessment
2.9. Statistical Analysis
3. Results
3.1. Micro-CT
3.2. Histochemical Staining
3.2.1. H&E Staining
3.2.2. IHC Staining
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Components (wt%) | Size (mm) Av. ± SD | Porosity (%) Av. ± S.D. | Weight (g) Av. ± SD | Density (g/cm3) Av. ± SD | |||
---|---|---|---|---|---|---|---|
β-TCP | PDLLA | Diameter | Thickness | ||||
β-TCP/PDLLA | 70 | 30 | 4.0 ± 0.2 | 2.0 ± 0.2 | 70 ± 5 | 0.017 ± 0.001 | 0.68 ± 0.04 |
β-TCP | 100 | 0 | 4.0 ± 0.2 | 2.0 ± 0.2 | 77.5 ± 4.5 | 0.016 ± 0.001 | 0.69 ± 0.06 |
PDLLA | 0 | 100 | 4.0 ± 0.2 | 2.0 ± 0.2 | 70 ± 5 | 0.008 ± 0.001 | 0.37 ± 0.03 |
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Toda, E.; Bai, Y.; Sha, J.; Dong, Q.N.; Ngo, H.X.; Suyama, T.; Miyamoto, K.; Matsuzaki, Y.; Kanno, T. Feasibility of Application of the Newly Developed Nano-Biomaterial, β-TCP/PDLLA, in Maxillofacial Reconstructive Surgery: A Pilot Rat Study. Nanomaterials 2021, 11, 303. https://doi.org/10.3390/nano11020303
Toda E, Bai Y, Sha J, Dong QN, Ngo HX, Suyama T, Miyamoto K, Matsuzaki Y, Kanno T. Feasibility of Application of the Newly Developed Nano-Biomaterial, β-TCP/PDLLA, in Maxillofacial Reconstructive Surgery: A Pilot Rat Study. Nanomaterials. 2021; 11(2):303. https://doi.org/10.3390/nano11020303
Chicago/Turabian StyleToda, Erina, Yunpeng Bai, Jingjing Sha, Quang Ngoc Dong, Huy Xuan Ngo, Takashi Suyama, Kenichi Miyamoto, Yumi Matsuzaki, and Takahiro Kanno. 2021. "Feasibility of Application of the Newly Developed Nano-Biomaterial, β-TCP/PDLLA, in Maxillofacial Reconstructive Surgery: A Pilot Rat Study" Nanomaterials 11, no. 2: 303. https://doi.org/10.3390/nano11020303