Safety of Lavender Oil-Loaded Niosomes for In Vitro Culture and Biomedical Applications
Abstract
:1. Introduction
2. Materials and Methods
2.1. Niosomal Lavender Oil Preparation Method
2.2. LON Characterization
2.3. Loading Efficiency and Loading Capacity of Lavender Oil
2.4. LON Storage Stability Study
2.5. LON Release Study
2.6. Cell Culture
2.7. Cell Viability
2.8. Statistical Analysis
3. Results
3.1. LON Characterization, Storage Stability, and Release Profile
3.2. Cytotoxicity
4. Discussion
5. Conclusions
Author Contributions
Funding
Data Availability Statement
Conflicts of Interest
References
- Fakari, F.R.; Tabatabaeichehr, M.; Kamali, H.; Fakari, F.R.; Naseri, M. Effect of Inhalation of Aroma of Geranium Essence on Anxiety and Physiological Parameters during First Stage of Labor in Nulliparous Women: A Randomized Clinical Trial. J. Caring Sci. 2015, 4, 135–141. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Santos, F.; Rao, V.S. Antiinflammatory and antinociceptive effects of 1,8-cineole a terpenoid oxide present in many plant essential oils. Phytother. Res. 2000, 14, 240–244. [Google Scholar] [CrossRef]
- Koulivand, P.H.; Ghadiri, M.K.; Gorji, A. Lavender and the Nervous System. Evid.-Based Complement. Altern. Med. 2013, 2013, 681304. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Ghods, A.A.; Abforosh, N.H.; Ghorbani, R.; Asgari, M.R. The effect of topical application of lavender essential oil on the intensity of pain caused by the insertion of dialysis needles in hemodialysis patients: A randomized clinical trial. Complement. Ther. Med. 2015, 23, 325–330. [Google Scholar] [CrossRef] [PubMed]
- Hajiali, H.; Summa, M.; Russo, D.; Armirotti, A.; Brunetti, V.; Bertorelli, R.; Athanassiou, A.; Mele, E. Alginate–lavender nanofibers with antibacterial and anti-inflammatory activity to effectively promote burn healing. J. Mater. Chem. B 2016, 4, 1686–1695. [Google Scholar] [CrossRef] [Green Version]
- Sayorwan, W.; Siripornpanich, V.; Piriyapunyaporn, T.; Hongratanaworakit, T.; Kotchabhakdi, N.; Ruangrungsi, N. The effects of lavender oil inhalation on emotional states, autonomic nervous system, and brain electrical activity. J. Med. Assoc. Thail. 2012, 95, 598–606. [Google Scholar]
- Cavanagh, H.M.A.; Wilkinson, J.M. Biological activities of Lavender essential oil. Phytother. Res. 2002, 16, 301–308. [Google Scholar] [CrossRef]
- Balasubramanian, K.; Kodam, K.M. Encapsulation of therapeutic lavender oil in an electrolyte assisted polyacrylonitrile nanofibres for antibacterial applications. RSC Adv. 2014, 4, 54892–54901. [Google Scholar] [CrossRef]
- Aboutaleb, N.; Jamali, H.; Abolhasani, M.; Toroudi, H.P. Lavender oil (Lavandula angustifolia) attenuates renal ischemia/reperfusion injury in rats through suppression of inflammation, oxidative stress and apoptosis. Biomed. Pharmacother. 2018, 110, 9–19. [Google Scholar] [CrossRef]
- Souri, F.; Rakhshan, K.; Erfani, S.; Azizi, Y.; Maleki, S.N.; Aboutaleb, N. Natural lavender oil (Lavandula angustifolia) exerts cardioprotective effects against myocardial infarction by targeting inflammation and oxidative stress. Inflammopharmacology 2018, 27, 799–807. [Google Scholar] [CrossRef]
- Vakili, A.; Sharifat, S.; Akhavan, M.M.; Bandegi, A.R. Effect of lavender oil (Lavandula angustifolia) on cerebral edema and its possible mechanisms in an experimental model of stroke. Brain Res. 2014, 1548, 56–62. [Google Scholar] [CrossRef] [PubMed]
- Mohanraj, V.; Chen, Y. Nanoparticles-a review. Trop. J. Pharm. Res. 2006, 5, 561–573. [Google Scholar] [CrossRef] [Green Version]
- Campbell, F.W.; Compton, R.G. The use of nanoparticles in electroanalysis: An updated review. Anal. Bioanal. Chem. 2009, 396, 241–259. [Google Scholar] [CrossRef]
- Moghassemi, S.; Hadjizadeh, A. Nano-niosomes as nanoscale drug delivery systems: An illustrated review. J. Control. Release 2014, 185, 22–36. [Google Scholar] [CrossRef] [PubMed]
- Moghassemi, S.; Parnian, E.; Hakamivala, A.; Darzianiazizi, M.; Vardanjani, M.M.; Kashanian, S.; Larijani, B.; Omidfar, K. Uptake and transport of insulin across intestinal membrane model using trimethyl chitosan coated insulin niosomes. Mater. Sci. Eng. C 2015, 46, 333–340. [Google Scholar] [CrossRef] [PubMed]
- Verma, S.; Singh, S.; Syan, N.; Mathur, P.; Valecha, V. Nanoparticle vesicular systems: A versatile tool for drug delivery. J. Chem. Pharm. Res. 2010, 2, 496–509. [Google Scholar]
- Zhang, Y.; Xia, Q.; Wu, T.; He, Z.; Li, Y.; Li, Z.; Hou, X.; He, Y.; Ruan, S.; Wang, Z. A novel multi-functionalized multicellular nanodelivery system for non-small cell lung cancer photochemotherapy. J. Nanobiotechnol. 2021, 19, 245. [Google Scholar] [CrossRef]
- Wu, T.; Zhu, C.; Wang, X.; Kong, Q.; Guo, T.; He, Z.; He, Y.; Ruan, S.; Ruan, H.; Pei, L.; et al. Cholesterol and Phospholipid-free Multilamellar Niosomes Regulate Transdermal Permeation of a Hydrophobic Agent Potentially Administrated for Treating Diseases in Deep Hair Follicles. J. Pharm. Sci. 2022, 111, 1785–1797. [Google Scholar] [CrossRef]
- Zhang, Y.T.; Jing, Q.; Hu, H.; He, Z.; Wu, T.; Guo, T.; Feng, N. Sodium dodecyl sulfate improved stability and transdermal delivery of salidroside-encapsulated niosomes via effects on zeta potential. Int. J. Pharm. 2020, 580, 119183. [Google Scholar] [CrossRef]
- Dominici, M.; Le Blanc, K.; Mueller, I.; Slaper-Cortenbach, I.; Marini, F.C.; Krause, D.S.; Deans, R.J.; Keating, A.; Prockop, D.J.; Horwitz, E.M. Minimal criteria for defining multipotent mesenchymal stromal cells. The International Society for Cellular Therapy position statement. Cytotherapy 2006, 8, 315–317. [Google Scholar] [CrossRef]
- Manavella, D.; Cacciottola, L.; Desmet, C.M.; Jordan, B.F.; Donnez, J.; A Amorim, C.; Dolmans, M.M. Adipose tissue-derived stem cells in a fibrin implant enhance neovascularization in a peritoneal grafting site: A potential way to improve ovarian tissue transplantation. Hum. Reprod. 2018, 33, 270–279. [Google Scholar] [CrossRef] [PubMed]
- Manavella, D.; Cacciottola, L.; Payen, V.L.; A Amorim, C.; Donnez, J.; Dolmans, M.M. Adipose tissue-derived stem cells boost vascularization in grafted ovarian tissue by growth factor secretion and differentiation into endothelial cell lineages. Mol. Hum. Reprod. 2019, 25, 184–193. [Google Scholar] [CrossRef] [PubMed]
- Manavella, D.; Cacciottola, L.; Pommé, S.; Desmet, C.M.; Jordan, B.F.; Donnez, J.; A Amorim, C.; Dolmans, M.M. Two-step transplantation with adipose tissue-derived stem cells increases follicle survival by enhancing vascularization in xenografted frozen–thawed human ovarian tissue. Hum. Reprod. 2018, 33, 1107–1116. [Google Scholar] [CrossRef] [Green Version]
- Yong, K.W.; Pingguan-Murphy, B.; Xu, F.; Abas, W.A.B.W.; Choi, J.R.; Omar, S.Z.; Azmi, M.A.N.; Chua, K.H.; Safwani, W.K.Z.W. Phenotypic and Functional Characterization of Long-Term Cryopreserved Human Adipose-derived Stem Cells. Sci. Rep. 2015, 5, 9596. [Google Scholar] [CrossRef] [Green Version]
- Hinz, B.; Phan, S.H.; Thannickal, V.J.; Galli, A.; Bochaton-Piallat, M.-L.; Gabbiani, G. The Myofibroblast: One Function, Multiple Origins. Am. J. Pathol. 2007, 170, 1807–1816. [Google Scholar] [CrossRef] [PubMed]
- Hinz, B. The myofibroblast: Paradigm for a mechanically active cell. J. Biomech. 2010, 43, 146–155. [Google Scholar] [CrossRef] [PubMed]
- Mosher, A.A.; Rainey, K.J.; Bolstad, S.S.; Lye, S.J.; Mitchell, B.F.; Olson, D.M.; Wood, S.L.; Slater, D.M. Development and validation of primary human myometrial cell culture models to study pregnancy and labour. BMC Pregnancy Childbirth 2013, 13, S7. [Google Scholar] [CrossRef] [Green Version]
- Vallet-Strouve, C.; Mowszowicz, I. Myometrial cells in primary culture: Characterization and hormonal profile. Mol. Cell. Endocrinol. 1978, 12, 97–110. [Google Scholar] [CrossRef]
- Dadashzadeh, A.; Imani, R.; Moghassemi, S.; Omidfar, K.; Abolfathi, N. Study of hybrid alginate/gelatin hydrogel-incorporated niosomal Aloe vera capable of sustained release of Aloe vera as potential skin wound dressing. Polym. Bull. 2019, 77, 387–403. [Google Scholar] [CrossRef]
- Moghassemi, S.; Hadjizadeh, A.; Hakamivala, A.; Omidfar, K. Growth Factor-Loaded Nano-niosomal Gel Formulation and Characterization. AAPS PharmSciTech 2016, 18, 34–41. [Google Scholar] [CrossRef]
- Moghassemi, S.; Hadjizadeh, A.; Omidfar, K. Formulation and Characterization of Bovine Serum Albumin-Loaded Niosome. AAPS PharmSciTech 2017, 18, 27–33. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Nguyen-Thi, N.-T.; Tran, L.P.P.; Le, N.T.T.; Cao, M.-T.; Tran, T.-N.; Nguyen, N.T.; Nguyen, C.H.; Nguyen, D.-H.; Than, V.T.; Le, Q.T.; et al. The Engineering of Porous Silica and Hollow Silica Nanoparticles to Enhance Drug-loading Capacity. Processes 2019, 7, 805. [Google Scholar] [CrossRef] [Green Version]
- Wang, Z.; Ling, L.; Du, Y.; Yao, C.; Li, X. Reduction responsive liposomes based on paclitaxel-ss-lysophospholipid with high drug loading for intracellular delivery. Int. J. Pharm. 2019, 564, 244–255. [Google Scholar] [CrossRef] [PubMed]
- Miyazaki, K.; Maruyama, T. Partial regeneration and reconstruction of the rat uterus through recellularization of a decellularized uterine matrix. Biomaterials 2014, 35, 8791–8800. [Google Scholar] [CrossRef]
- Ono, M.; Kajitani, T.; Uchida, H.; Arase, T.; Oda, H.; Uchida, S.; Ota, K.; Nagashima, T.; Masuda, H.; Miyazaki, K.; et al. CD34 and CD49f Double-Positive and Lineage Marker-Negative Cells Isolated from Human Myometrium Exhibit Stem Cell-Like Properties Involved in Pregnancy-Induced Uterine Remodeling. Biol. Reprod. 2015, 93, 37. [Google Scholar] [CrossRef] [Green Version]
- Gu, Q.; Tomaskovic-Crook, E.; Wallace, G.G.; Crook, J.M. 3D bioprinting human induced pluripotent stem cell constructs for in situ cell proliferation and successive multilineage differentiation. Adv. Healthc. Mater. 2017, 6, 1700175. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Amorim, C.; Van Langendonckt, A.; David, A.; Dolmans, M.-M.; Donnez, J. Survival of human pre-antral follicles after cryopreservation of ovarian tissue, follicular isolation and in vitro culture in a calcium alginate matrix. Hum. Reprod. 2008, 24, 92–99. [Google Scholar] [CrossRef] [Green Version]
- Asaithambi, K.; Muthukumar, J.; Chandrasekaran, R.; Ekambaram, N.; Roopan, S.M. Synthesis and characterization of turmeric oil loaded non-ionic surfactant vesicles (niosomes) and its enhanced larvicidal activity against mosquito vectors. Biocatal. Agric. Biotechnol. 2020, 29, 101737. [Google Scholar] [CrossRef]
- Yeo, L.K.; Olusanya, T.O.B.; Chaw, C.S.; Elkordy, A.A. Brief Effect of a Small Hydrophobic Drug (Cinnarizine) on the Physicochemical Characterisation of Niosomes Produced by Thin-Film Hydration and Microfluidic Methods. Pharmaceutics 2018, 10, 185. [Google Scholar] [CrossRef] [Green Version]
- Van Vuuren, S.F.; du Toit, L.; Parry, A.; Pillay, V.; Choonara, Y. Encapsulation of Essential Oils within a Polymeric Liposomal Formulation for Enhancement of Antimicrobial Efficacy. Nat. Prod. Commun. 2010, 5, 1401–1408. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Clogston, J.D.; Patri, A.K. Zeta potential measurement. In Characterization of Nanoparticles Intended for Drug Delivery; Springer: Berlin/Heidelberg, Germany, 2011; pp. 63–70. [Google Scholar]
- Rouholamini, S.E.Y.; Moghassemi, S.; Maharat, Z.; Hakamivala, A.; Kashanian, S.; Omidfar, K. Effect of silibinin-loaded nano-niosomal coated with trimethyl chitosan on miRNAs expression in 2D and 3D models of T47D breast cancer cell line. Artif. Cells Nanomed. Biotechnol. 2017, 46, 524–535. [Google Scholar] [CrossRef]
- Junyaprasert, V.B.; Teeranachaideekul, V.; Supaperm, T. Effect of Charged and Non-ionic Membrane Additives on Physicochemical Properties and Stability of Niosomes. AAPS PharmSciTech 2008, 9, 851–859. [Google Scholar] [CrossRef] [PubMed]
- Nelson, R.R. In-vitro activities of five plant essential oils against methicillin-resistant Staphylococcus aureus and vancomycin-resistant Enterococcus faecium. J. Antimicrob. Chemother. 1997, 40, 305–306. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Inouye, S.; Watanabe, M.; Nishiyama, Y.; Takeo, K.; Akao, M.; Yamaguchi, H. Antisporulating and respiration-inhibitory effects of essential oils on filamentous fungi. Mycoses 1998, 41, 403–410. [Google Scholar] [CrossRef] [PubMed]
- Hakamivala, A.; Moghassemi, S.; Omidfar, K. Modeling and optimization of the niosome nanovesicles using response surface methodology for delivery of insulin. Biomed. Phys. Eng. Express 2019, 5, 045041. [Google Scholar] [CrossRef]
Time (Months) | Size (nm) | Polydispersity Index | Zeta Potential (mV) | LE (%) | LC (%) |
---|---|---|---|---|---|
0 | 1216 ± 106 | 0.328 ± 0.045 | −22.4 ± 0.9 | 78.032 ± 2.141 | 28.35 ± 0.56 |
15 | 1142.5 ± 47.5 | 0.425 ± 0.035 | −24.55 ± 1.45 | 73.99 ± 4.35 | 27.26 ± 1.17 |
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Vilela, J.d.M.V.; Moghassemi, S.; Dadashzadeh, A.; Dolmans, M.-M.; Azevedo, R.B.; Amorim, C.A. Safety of Lavender Oil-Loaded Niosomes for In Vitro Culture and Biomedical Applications. Nanomaterials 2022, 12, 1999. https://doi.org/10.3390/nano12121999
Vilela JdMV, Moghassemi S, Dadashzadeh A, Dolmans M-M, Azevedo RB, Amorim CA. Safety of Lavender Oil-Loaded Niosomes for In Vitro Culture and Biomedical Applications. Nanomaterials. 2022; 12(12):1999. https://doi.org/10.3390/nano12121999
Chicago/Turabian StyleVilela, Janice de M. V., Saeid Moghassemi, Arezoo Dadashzadeh, Marie-Madeleine Dolmans, Ricardo B. Azevedo, and Christiani A. Amorim. 2022. "Safety of Lavender Oil-Loaded Niosomes for In Vitro Culture and Biomedical Applications" Nanomaterials 12, no. 12: 1999. https://doi.org/10.3390/nano12121999