Magnetic Levitation Patterns of Microfluidic-Generated Nanoparticle–Protein Complexes
Abstract
:1. Introduction
2. Materials and Methods
2.1. Preparation of Graphene Oxide
2.2. Preparation of GO-HP Samples
2.3. MagLev Device
2.4. Fundamentals of MagLev
2.5. Atomic Force Microscopy (AFM)
2.6. Bicinchoninic Acid Assay (BCA)
2.7. Sodium Dodecyl Sulphate–Polyacrylamide Gel Electrophoresis (SDS-PAGE)
2.8. Nanoliquid Chromatography–Tandem Mass Spectrometry
3. Results and Discussion
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Dynamic Incubation | Static Incubation | |||
---|---|---|---|---|
GO:HP FRR | GO Flow Rate (μL/min) | HP Flow Rate (μL/min) | GO Amount (μL) | HP Amount (μL) |
4:1 | 2.80 | 0.70 | 80 | 20 |
9:1 | 3.14 | 0.35 | 90 | 10 |
19:1 | 3.30 | 0.17 | 95 | 5 |
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Digiacomo, L.; Quagliarini, E.; Marmiroli, B.; Sartori, B.; Perini, G.; Papi, M.; Capriotti, A.L.; Montone, C.M.; Cerrato, A.; Caracciolo, G.; et al. Magnetic Levitation Patterns of Microfluidic-Generated Nanoparticle–Protein Complexes. Nanomaterials 2022, 12, 2376. https://doi.org/10.3390/nano12142376
Digiacomo L, Quagliarini E, Marmiroli B, Sartori B, Perini G, Papi M, Capriotti AL, Montone CM, Cerrato A, Caracciolo G, et al. Magnetic Levitation Patterns of Microfluidic-Generated Nanoparticle–Protein Complexes. Nanomaterials. 2022; 12(14):2376. https://doi.org/10.3390/nano12142376
Chicago/Turabian StyleDigiacomo, Luca, Erica Quagliarini, Benedetta Marmiroli, Barbara Sartori, Giordano Perini, Massimiliano Papi, Anna Laura Capriotti, Carmela Maria Montone, Andrea Cerrato, Giulio Caracciolo, and et al. 2022. "Magnetic Levitation Patterns of Microfluidic-Generated Nanoparticle–Protein Complexes" Nanomaterials 12, no. 14: 2376. https://doi.org/10.3390/nano12142376