Distinct Uptake Routes Participate in Silver Nanoparticle Engulfment by Earthworm and Human Immune Cells
Abstract
:1. Introduction
2. Materials and Methods
2.1. Nanoparticles
2.2. Earthworm Husbandry and Coelomocyte Isolations
2.3. Cell Culture Conditions
2.4. Endocytosis Inhibitor Treatments and In Vitro Exposure Conditions
2.5. Flow Cytometry Measurement of SSC Parameters
2.6. Transmission Electron Microscopy (TEM) for Intracellular AgNP Localization
2.7. Quantification of Intracellular Silver Content by Inductively Coupled Plasma Mass Spectrometry (ICP-MS)
2.8. RNA Isolation, cDNA Synthesis, and Real-Time PCR
2.9. Quantification of Global Genomic DNA Methylation Levels
2.10. Bioenergetic Analysis of Mitochondria
2.11. Statistical Analysis
3. Results and Discussion
3.1. Physico-Chemical Characterization of AgNPs
3.2. Earthworm and Human Immunocytes Possess Different Sensitivities towards AgNPs
3.3. Human THP-1 and Diff. THP-1 Cells Utilize Similar Endocytosis Mechanisms for AgNP Uptake
3.4. Earthworm Coelomocytes Rely on Actin-Dependent Phagocytosis for AgNPs Uptake
3.5. AgNPs Do Not Enter the Target Cell Nuclei
3.6. Quantification of Intracellular Silver Content
3.7. TLR/MyD88 Signaling Facilitates AgNPs Internalization
3.8. Earthworm Coelomocytes, but Not Human Immunocytes, Exhibit Global DNA Hypermethylation upon AgNPs’ Uptake
3.9. Cellular Respiration Parameters Changed in Response to AgNPs
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Inhibitor | Target Pathway | Mode of Action | Applied Conditions | References |
---|---|---|---|---|
Cytochalasin B | Phagocytosis | F-actin depolymerization | 5 µM, 1 h | [22] |
Cytochalasin D | 5 µM, 1 h | [23] | ||
Colchicine | Pinocytosis | Disrupting microtubules | 100 µM, 2 h | [24] |
5-(N-ethyl-N-isopropyl) amiloride (EIPA) | Macropinocytosis | Blocking the Na+/H+ exchanger | 5 µM, 30 min | [25] |
Amantadine | Clathrin-mediated endocytosis (CME) | Blocks the clathrin-coated pits | 500 µM, 30 min | [26] |
Methyl-ß-cyclodextrin (MßCD) | Caveolae-mediated endocytosis (CvME) | Remove cholesterol from membrane | 1 mM, 30 min | [27] |
Nystatin | CvME | Cholesterol sequestration | 54 µM, 30 min | [28] |
Polyinosinic acid (Poly(I)) | Various pathways (Scavenger receptor (ScR)-mediated) | Binds SR | 287.2 µM, 1 h | [29] |
Polycytidylic acid (PolyI) | None (Poly(I) antagonist) | Does not bind SR | 107.1 µM, 1 h | [30] |
Wortmannin | Macropinocytosis | PI3K inhibition | 400 nM, 30 min | [31] |
% Total Silver Mass in Cells | Ø Inhibitor | Poly(I) | Colchicine | Wortmannin | Cyt. B | Cyt. D |
---|---|---|---|---|---|---|
THP-1 | 19.72 ± 0.48 | 11.05 ± 7.99 | 8.94 ± 1.02 | 10.34 ± 3.39 | - | - |
diff. THP-1 | 17.34 ± 10.11 | 12.51 ± 9.61 | 11.38 ± 10.55 | 13.3 ± 2.99 | - | - |
Coelomocytes | 16.22 ± 0.4 | - | - | - | 16.62 ± 2.2 | 19.62 ± 0.73 |
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Kokhanyuk, B.; Vántus, V.B.; Radnai, B.; Vámos, E.; Kajner, G.; Galbács, G.; Telek, E.; Mészáros, M.; Deli, M.A.; Németh, P.; et al. Distinct Uptake Routes Participate in Silver Nanoparticle Engulfment by Earthworm and Human Immune Cells. Nanomaterials 2022, 12, 2818. https://doi.org/10.3390/nano12162818
Kokhanyuk B, Vántus VB, Radnai B, Vámos E, Kajner G, Galbács G, Telek E, Mészáros M, Deli MA, Németh P, et al. Distinct Uptake Routes Participate in Silver Nanoparticle Engulfment by Earthworm and Human Immune Cells. Nanomaterials. 2022; 12(16):2818. https://doi.org/10.3390/nano12162818
Chicago/Turabian StyleKokhanyuk, Bohdana, Viola Bagóné Vántus, Balázs Radnai, Eszter Vámos, Gyula Kajner, Gábor Galbács, Elek Telek, Mária Mészáros, Mária A. Deli, Péter Németh, and et al. 2022. "Distinct Uptake Routes Participate in Silver Nanoparticle Engulfment by Earthworm and Human Immune Cells" Nanomaterials 12, no. 16: 2818. https://doi.org/10.3390/nano12162818