Construction of N-CDs and Calcein-Based Ratiometric Fluorescent Sensor for Rapid Detection of Arginine and Acetaminophen
Abstract
:1. Introduction
2. Experimental Part
2.1. Materials
2.2. Experimental Instruments
2.3. Synthesis of N-CDs
2.4. Quantum Yield of N-CDs
2.5. Construction of N-CD/Calcein Ratiometric Fluorescent Sensor
2.6. Stability Evaluation
2.7. Characteristics of N-CD/Calcein Sensor in Arg and AP Detection
3. Results and Discussion
3.1. Characterization of N-CDs
3.2. Optical Properties of N-CDs and Calcein
3.3. Construction of N-CD/Calcein Ratiometric Fluorescent Sensor
3.4. Characteristics of N-CD/Calcein Ratiometric Fluorescent Sensor
3.5. Ratiometric Fluorescence and Visual Detection of Arg and AP
3.6. Mechanism of the Ratiometric Sensing System
3.7. Detection of Arg and AP in Bovine Serum
3.8. Logic Gate Application and Reversibility of N-CD/Calcein Sensor
4. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Sensor | Linear Range | LOD | Ref. |
---|---|---|---|
Mg-CDs | 0.3–130 μM | 0.15 μM | [25] |
DNA-CuNC/CDs | 0–100 μM | 0.35 μM | [38] |
Conductometric biosensor | −2.5–500 μM | −2.5 μM | [54] |
AuNPs | 1–6 μM | 10 μM | [55] |
Citrate-capped AuNPs | 0.08–13.2 μM | 0.02 μM | [56] |
Capillary | 30–1500 μM | 10 μM | [57] |
N-CDs/calcein | 0.1–100 μM | 0.08 μM | This work |
Sensor | Linear Range | LOD | Ref. |
---|---|---|---|
Ni2P nanosheets/GCE | 0.5~4500 μM | 0.11 μM | [58] |
DNA-CuNC/CDs | 0–100 μM | 0.26 μM | [38] |
Mo2C@BMZIFs/GCE | 0.1–300 μM | 0.03 μM | [59] |
P-NC/GCE | 3–110 μM | 0.5 μM | [60] |
ZnO-MoO3-C/GCE | 2.5–2000 μM | 1.14 μM | [61] |
N-CDs/calcein/Arg | 0.03–3 μM | 0.02 μM | This Work |
Sample | Found in Sample (μM) | Added (μM) | Measured (μM) | Recovery (%) | RSD (%) |
---|---|---|---|---|---|
Serum | 70.82 | 5 | 76.04 | 104.4 | 0.7 |
72.04 | 15 | 86.37 | 95.5 | 0.2 | |
70.91 | 25 | 96.07 | 100.6 | 0.4 |
Sample | Found in Sample (μM) | Added (μM) | Measured (μM) | Recovery (%) | RSD (%) |
---|---|---|---|---|---|
Serum | 0 | 0.5 | 0.51 | 102.0 | 0.8 |
0 | 1.5 | 1.49 | 99.3 | 1.9 | |
0 | 2.5 | 2.49 | 99.6 | 2.7 |
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Qi, H.; Li, Q.; Jing, J.; Jing, T.; Liu, C.; Qiu, L.; Sami, R.; Helal, M.; Ismail, K.A.; Aljahani, A.H. Construction of N-CDs and Calcein-Based Ratiometric Fluorescent Sensor for Rapid Detection of Arginine and Acetaminophen. Nanomaterials 2022, 12, 976. https://doi.org/10.3390/nano12060976
Qi H, Li Q, Jing J, Jing T, Liu C, Qiu L, Sami R, Helal M, Ismail KA, Aljahani AH. Construction of N-CDs and Calcein-Based Ratiometric Fluorescent Sensor for Rapid Detection of Arginine and Acetaminophen. Nanomaterials. 2022; 12(6):976. https://doi.org/10.3390/nano12060976
Chicago/Turabian StyleQi, Haiyan, Qiuying Li, Jing Jing, Tao Jing, Chuntong Liu, Lixin Qiu, Rokayya Sami, Mahmoud Helal, Khadiga Ahmed Ismail, and Amani H. Aljahani. 2022. "Construction of N-CDs and Calcein-Based Ratiometric Fluorescent Sensor for Rapid Detection of Arginine and Acetaminophen" Nanomaterials 12, no. 6: 976. https://doi.org/10.3390/nano12060976