CeO2 Nanoparticles-Regulated Plasmid Uptake and Bioavailability for Reducing Transformation of Extracellular Antibiotic Resistance Genes
Abstract
:1. Introduction
2. Materials and Methods
2.1. Plasmid Extraction, Bacterial Strains and Characterization of CeO2 NPs
2.2. Establishment of Transformation Systems and Measurement of Transformation Efficiency
2.3. Identification of Transformants
2.4. FTIR Analysis and Quantification of Plasmids after Binding with CeO2 NPs
2.5. Measurements of ROS and Cell Membrane Permeability
2.6. Measurement of CeO2 NPs Internalized by E. coli Cells
2.7. Relative Expression of Transformation-Related Genes Determined by qRT-PCR
2.8. Statistical Analysis
3. Results and Discussion
3.1. Effects of CeO2 NPs on the Transformation of eDNA
3.2. Interactions between Plasmid and CeO2 NPs
3.3. Regulation of ROS Generation and Cell Membrane Permeability by CeO2 NPs
3.4. Expression of Transformation-Related Genes Altered by CeO2 NPs
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Xu, Y.; Du, H.; Wang, C.; Yue, L.; Chen, F.; Wang, Z. CeO2 Nanoparticles-Regulated Plasmid Uptake and Bioavailability for Reducing Transformation of Extracellular Antibiotic Resistance Genes. Nanomaterials 2023, 13, 969. https://doi.org/10.3390/nano13060969
Xu Y, Du H, Wang C, Yue L, Chen F, Wang Z. CeO2 Nanoparticles-Regulated Plasmid Uptake and Bioavailability for Reducing Transformation of Extracellular Antibiotic Resistance Genes. Nanomaterials. 2023; 13(6):969. https://doi.org/10.3390/nano13060969
Chicago/Turabian StyleXu, Yinuo, Hao Du, Chuanxi Wang, Le Yue, Feiran Chen, and Zhenyu Wang. 2023. "CeO2 Nanoparticles-Regulated Plasmid Uptake and Bioavailability for Reducing Transformation of Extracellular Antibiotic Resistance Genes" Nanomaterials 13, no. 6: 969. https://doi.org/10.3390/nano13060969