Human Serum Albumin Protein Corona in Prussian Blue Nanoparticles
Abstract
:1. Introduction
2. Materials and Methods
2.1. Materials
2.2. Synthesis of PBnps
2.3. Synthesis of HSA-Coated PBnps (PBnp@HSA)
2.4. Phosphate Buffer at pH 7.4
2.5. PBnp@HSA Stability in Phosphate Buffer at pH 7.4
2.6. PBnp Stability in Serum-like Conditions
2.7. pH-Spectrophotometric Titration of PBnp@HSA
2.8. Quantitative Determination of HSA with Folin Reagent
2.8.1. Calibration Curve
2.8.2. Determination of HSA
2.9. Determination of the PBnp Yield
2.10. Instrumentation
2.11. Statistical Analysis
3. Results and Discussion
3.1. Synthesis of PBnps and Their HSA Coating
3.2. Spectral Changes with pH in PBnp@HSA
3.3. Stability vs. Decomposition of PBnp@HSA at pH 7.4 as a Function of CHSA
3.4. Quantitative Determination of HSA Bound to PBnps
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Solution E (mL) | Bidistilled Water (mL) | Solution D (mL) | 1 N Folin Reagent (mL) | HSA Concentration (μg/mL) | |
---|---|---|---|---|---|
blank | 0.00 | 5.00 | 0.70 | 0.10 | 0 |
Std 1 | 0.10 | 4.90 | 0.70 | 0.10 | 3.59 |
Std 2 | 0.30 | 4.70 | 0.70 | 0.10 | 10.76 |
Std 3 | 0.50 | 4.50 | 0.70 | 0.10 | 17.93 |
Std 4 | 1.00 | 4.00 | 0.70 | 0.10 | 35.86 |
Std 5 | 1.50 | 3.50 | 0.70 | 0.10 | 53.79 |
Std 6 | 2.00 | 3.00 | 0.70 | 0.10 | 71.72 |
Std 7 | 2.50 | 2.50 | 0.70 | 0.10 | 89.65 |
Std 8 | 3.00 | 2.00 | 0.70 | 0.10 | 107.59 |
CHSA (mg/mL) | dh (nm) | PDI 2 |
---|---|---|
0.025 | 320(8) | 0.37(0.01) |
0.050 | 291(3) | 0.23(0.03) |
0.10 | 240(1) | 0.21(0.04) |
0.25 | 183(1) | 0.18(0.02) |
0.50 | 176(2) | 0.17(0.02) |
1.0 | 142(1) | 0.19(0.02) |
3.0 | 123(2) | 0.22(0.01) |
5.0 | 112(2) | 0.20(0.01) |
7.0 | 113(2) | 0.21(0.02) |
CHSA (μg/mL) | p-CHSA a (μg/mL) | s-CHSA a (μg/mL) | % HSAE/C |
---|---|---|---|
25 | 48(6) | 4.4(0.6) | 210 |
50 | 58(5) | 9(3) | 134 |
100 | 72(6) | 35(6) | 107 |
250 | 89(2) | 162(12) | 100 |
500 | 78(7) | 406(18) | 97 |
1000 | 118(12) | 899(103) | 102 |
3000 | 121(33) | 2566(101) | 90 |
5000 | 164(11) | 4574(471) | 95 |
7000 | 182(7) | 6627(390) | 97 |
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Colombi, C.; Dacarro, G.; Diaz Fernandez, Y.A.; Taglietti, A.; Pallavicini, P.; Doveri, L. Human Serum Albumin Protein Corona in Prussian Blue Nanoparticles. Nanomaterials 2024, 14, 1336. https://doi.org/10.3390/nano14161336
Colombi C, Dacarro G, Diaz Fernandez YA, Taglietti A, Pallavicini P, Doveri L. Human Serum Albumin Protein Corona in Prussian Blue Nanoparticles. Nanomaterials. 2024; 14(16):1336. https://doi.org/10.3390/nano14161336
Chicago/Turabian StyleColombi, Chiara, Giacomo Dacarro, Yuri Antonio Diaz Fernandez, Angelo Taglietti, Piersandro Pallavicini, and Lavinia Doveri. 2024. "Human Serum Albumin Protein Corona in Prussian Blue Nanoparticles" Nanomaterials 14, no. 16: 1336. https://doi.org/10.3390/nano14161336