Anti-Inflammatory and Antimicrobial Activity of Silver Nanoparticles Green-Synthesized Using Extracts of Different Plants
Abstract
:1. Introduction
2. Materials and Methods
2.1. Instrumentation
2.2. Materials and Reagents
2.3. Methods
2.3.1. Preparation of Plant Powders
2.3.2. Preparation of Plant Extracts
2.4. Synthesis of Silver Nanoparticles
2.5. Loading of AgNPs with an Anti-Inflammatory Drug
2.6. Anti-Inflammatory Activity of Tenoxicam/Meloxicam-Loaded AgNPs
2.7. Antimicrobial Activity of AgNPs
3. Results and Discussions
3.1. Characterization of Silver Nanoparticles
3.1.1. UV–Visible Spectroscopic Analysis of AgNPs
3.1.2. Morphological Study Using SEM
3.1.3. Fourier-Transform Infrared Spectroscopy (FT-IR)
3.2. Effect of Reaction Time
3.3. Effect of Extract and Silver Ion Volumes
3.4. In Vitro Anti-Inflammatory Activity of Tenoxicam/Meloxicam-Loaded AgNPs
3.5. Antimicrobial Activity
4. Conclusions
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Functional Group | GB-AgNPs | CI-AgNPs | AC-AgNPs | RO-AgNPs |
---|---|---|---|---|
(N-H) Stretching | 3350 cm−1 | 3390 cm−1 | 3384 cm−1 | 3406 cm−1 |
(O-H) Stretching | 3175 cm−1 | 3181 cm−1 | 3200 cm−1 | 3214 cm−1 |
(C-H) Stretching | 2920 cm−1 | 2920 cm−1 | 2850 cm−1 | 2950 cm−1 |
(C=O) Stretching | 1631 cm−1 | 1627 cm−1 | 1644 cm−1 | 1632 cm−1 |
(C=C) Stretching | 1540 cm−1 | 1475 cm−1 | 1644 cm−1 | 1632 cm−1 |
(C-O) Stretching | 1446 cm−1 | 1445 cm−1 | 1442 cm−1 | 1443 cm−1 |
(C-N) Stretching | 1369 cm−1 | 1364 cm−1 | 1371 cm−1 | 1361 cm−1 |
Aromatic C-H Bending | 881–672 cm−1 | 880–672 cm−1 | 880–671 cm−1 | 880–671 cm−1 |
Material | Tenoxicam | TNX-Loaded AgNPs | |||
---|---|---|---|---|---|
GB-AgNPs | CI-AgNPs | AC-AgNPs | RO-AgNPs | ||
% Inhibition | 29.9489 | 42.1456 | 62.5798 | 32.9502 | 50.9578 |
Material | Meloxicam | MLX-Loaded AgNPs | |||
GB-AgNPs | CI-AgNPs | AC-AgNPs | RO-AgNPs | ||
% Inhibition | 50.5108 | 57.4074 | 2.5543 | 27.8416 | 7.6628 |
Test Microorganism | Control Gentamycin | AgNPs Samples | |||
---|---|---|---|---|---|
GB-AgNPs | CI-AgNPs | AC-AgNPs | RO-AgNPs | ||
Gram-Positive bacteria: Staphylococcus aureus ATCC 25923 | 27 | 14 | 16 | 16 | 15 |
Gram-Negative bacteria: Escherichia coli ATCC 25922 | 31 | 18 | 19 | 18 | 19 |
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Mohamed, A.; Dayo, M.; Alahmadi, S.; Ali, S. Anti-Inflammatory and Antimicrobial Activity of Silver Nanoparticles Green-Synthesized Using Extracts of Different Plants. Nanomaterials 2024, 14, 1383. https://doi.org/10.3390/nano14171383
Mohamed A, Dayo M, Alahmadi S, Ali S. Anti-Inflammatory and Antimicrobial Activity of Silver Nanoparticles Green-Synthesized Using Extracts of Different Plants. Nanomaterials. 2024; 14(17):1383. https://doi.org/10.3390/nano14171383
Chicago/Turabian StyleMohamed, Amr, Marwa Dayo, Sana Alahmadi, and Samah Ali. 2024. "Anti-Inflammatory and Antimicrobial Activity of Silver Nanoparticles Green-Synthesized Using Extracts of Different Plants" Nanomaterials 14, no. 17: 1383. https://doi.org/10.3390/nano14171383