Properties of An Oral Nanoformulation of A Molecularly Dispersed Amphotericin B Comprising A Composite Matrix of Theobroma Oil and Bee’S Wax
Abstract
:1. Introduction
2. Experimental Section
2.1. Materials
2.2. Preparation of Lipid Nanoparticles
AmB (mg) | 20 mL of aqueous sodium cholate solution (% w/w) | Lecithin (% w/w of total lipid) | Lipid matrix (mg) | Water (mL) | |
---|---|---|---|---|---|
Theobroma oil | Beeswax | ||||
10.0; 35.0; 50.0; 65.0 | 5.0 | 30 | 200 | 200 | 80 |
2.3. Photon Correlation Spectroscopy Analysis
2.4. Encapsulation Efficiency
2.5. Nanoparticle Tracking Analysis (NTA)
2.6. Field Emission Scanning Electron Microscopy (FESEM) and Scanning Transmission Electron Microscopy (STEM) Analysis
2.7. Time-of-Flight Secondary Ion Mass Spectrometry (ToF-SIMS) Analysis
2.8. Statistical Analysis
3. Results and Discussion
3.1. Encapsulation Efficiency (%EE)
3.2. PCS Analysis
Formulation | z-average diameter (nm) | PDI | ξ (mV) |
---|---|---|---|
Drug-free nanoparticles | 169 ± 1 | 0.215 ± 0.023 | 40.8 ± 0.9 |
AmB-loaded nanoparticle | 222 ± 2 | 0.255 ± 0.006 | 50.3 ± 1.0 |
3.3. NTA Analysis
3.4. FESEM and STEM Analysis
3.5. Time of Flight Secondary Ion Mass Spectroscopy (ToF-SIMS) Analysis
4. Conclusions
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Tan, C.S.W.; Billa, N.; Roberts, C.J.; Scurr, D.J. Properties of An Oral Nanoformulation of A Molecularly Dispersed Amphotericin B Comprising A Composite Matrix of Theobroma Oil and Bee’S Wax. Nanomaterials 2014, 4, 905-916. https://doi.org/10.3390/nano4040905
Tan CSW, Billa N, Roberts CJ, Scurr DJ. Properties of An Oral Nanoformulation of A Molecularly Dispersed Amphotericin B Comprising A Composite Matrix of Theobroma Oil and Bee’S Wax. Nanomaterials. 2014; 4(4):905-916. https://doi.org/10.3390/nano4040905
Chicago/Turabian StyleTan, Chloe See Wei, Nashiru Billa, Clive J. Roberts, and David J. Scurr. 2014. "Properties of An Oral Nanoformulation of A Molecularly Dispersed Amphotericin B Comprising A Composite Matrix of Theobroma Oil and Bee’S Wax" Nanomaterials 4, no. 4: 905-916. https://doi.org/10.3390/nano4040905