Simultaneous Detection of Dopamine and Uric Acid Using a Poly(l-lysine)/Graphene Oxide Modified Electrode
Abstract
:1. Introduction
2. Results and Discussion
2.1. Fabrication and Characterization of Poly(l-lysine)/Graphene Oxide Modified Glassy Carbon Electrode (PLL/GO/GCE)
2.2. Electrochemical Behaviors of Uric Acid and Dopamine at Various Electrodes
2.3. Effective Surface Area of the PLL/GO/GCE
2.4. Influence of Scan Rate
2.5. Influence of pH
2.6. Optimization of Preparation Conditions of PLL/GO/GCE Sensor
2.7. Detection of UA and DA
2.7.1. Selective Detection
2.7.2. Simultaneous Detection
2.8. Reproducibility, Stability and Interferences
2.9. Analysis of Practical Samples
2.9.1. Determination of UA in Real Biological Samples
2.9.2. Determination of DA in Dopamine Hydrochloride Injection
3. Experimental Section
3.1. Reagents
3.2. Apparatus
3.3. Preparation of the Modified Electrodes
3.4. Experimental Methods
3.5. Sample Preparation
4. Conclusions
Supplementary Materials
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Electrode | Analyte | Linear Range (μM) | Detection Limit (μM) | Sensitivity (μA·cm−2·μM−1) | References |
---|---|---|---|---|---|
Nitrogen doped graphene/GCE | DA | 0.5–170 | 0.28 | 0.45 | [13] |
UA | 0.1–20 | 0.045 | 20.61 | ||
ERGOa/GCE | DA | 0.5–60 | 0.5 | 7.11 | [16] |
UA | 0.5–60 | 0.5 | 7.97 | ||
Poly(Vinyl alcohol) | DA | 2–70 | 1.4 | 13.52 | [24] |
UA | 2–50 | 0.6 | 43.82 | ||
Au/Reduced graphene oxide/GCE | DA | 6.8–41 | 1.4 | 14.08 | [17] |
UA | 8.8–53 | 1.8 | 7.04 | ||
PLL/GO/GCE | DA | 0.5–35 | 0.021 | 19.72 | This work |
UA | 0.5–20 20–200 | 0.074 | 11.50 |
Sample | Found (μM) | Added (μM) | Found (μM) | Recovery (%) | R.S.D (%) |
---|---|---|---|---|---|
Serum 1 | 48.26 | 10 | 58.4 | 101.4 | 2.4 |
Serum 2 | 56.57 | 20 | 75.87 | 96.5 | 3.1 |
Urine 1 | 45.17 | 20 | 64.30 | 95.65 | 1.4 |
Urine 2 | 40.46 | 15 | 56.05 | 103.9 | 1.8 |
DA Specified (μM) | Added (μM) | Found (μM) | Recovery (%) | R.S.D (%) |
---|---|---|---|---|
10.5 | 0 | 10.25 | - | 1.86 |
10.5 | 5 | 15.63 | 102.6 | 1.07 |
10.5 | 10 | 20.45 | 99.5 | 0.83 |
10.5 | 15 | 25.67 | 101.1 | 1.21 |
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Zhang, Y.; Lei, W.; Xu, Y.; Xia, X.; Hao, Q. Simultaneous Detection of Dopamine and Uric Acid Using a Poly(l-lysine)/Graphene Oxide Modified Electrode. Nanomaterials 2016, 6, 178. https://doi.org/10.3390/nano6100178
Zhang Y, Lei W, Xu Y, Xia X, Hao Q. Simultaneous Detection of Dopamine and Uric Acid Using a Poly(l-lysine)/Graphene Oxide Modified Electrode. Nanomaterials. 2016; 6(10):178. https://doi.org/10.3390/nano6100178
Chicago/Turabian StyleZhang, Yuehua, Wu Lei, Yujuan Xu, Xifeng Xia, and Qingli Hao. 2016. "Simultaneous Detection of Dopamine and Uric Acid Using a Poly(l-lysine)/Graphene Oxide Modified Electrode" Nanomaterials 6, no. 10: 178. https://doi.org/10.3390/nano6100178
APA StyleZhang, Y., Lei, W., Xu, Y., Xia, X., & Hao, Q. (2016). Simultaneous Detection of Dopamine and Uric Acid Using a Poly(l-lysine)/Graphene Oxide Modified Electrode. Nanomaterials, 6(10), 178. https://doi.org/10.3390/nano6100178