Antimicrobial Properties of Biofunctionalized Silver Nanoparticles on Clinical Isolates of Streptococcus mutans and Its Serotypes
Abstract
:1. Introduction
2. Results and Discussion
2.1. Characterization of BSA and CS Coated SNP
2.2. Antibacterial Test
2.3. Scanning Electron Microscopy
3. Materials and Methods
3.1. Materials and Reagents
3.2. Silver Nanoparticles Preparation
3.3. Preparation of BSA Coated Silver Nanoparticles
3.4. Preparation of CS Coated Silver Nanoparticles
3.5. Characterization
3.6. Identification of S. mutans Serotypes Using PCR
3.7. Antibacterial Assay
3.8. Statistical Analysis
4. Conclusions
Acknowledgments
Author Contributions
Conflicts of Interest
Abbreviations
µg/mL | microgram per milliliter |
BSA | Bovine serum albumin |
CFU | colony forming units |
CS | Chitosan |
DLS | Dynamic light scattering |
DSC | Differential scanning calorimetry |
MIC | Minimum inhibitory concentration |
S. mutans | Streptococcus mutans |
SEM | Scanning electron microscopy |
SNP | Silver nanoparticles |
TEM | Transmission electron microscopy |
TGA | Thermo-gravimetric analysis |
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SNP Sample (nm) | Diameter DLS (nm) | Zeta Potential (mV ± ZD) | Residues TGA (%) | Total Weight Loss TGA (%) |
---|---|---|---|---|
7.1 | 7.1 | −48.4 ± 6.9 | 98.5 | 1.5 |
17.4 | 17.4 | −52.6 ± 8.5 | 98.2 | 1.7 |
87.6 | 87.6 | −55.7 ± 9.9 | 97.8 | 2.1 |
BSA 16.5 | 16.5 | −13.5 ± 4.7 | 96.3 | 3.6 |
BSA 23.3 | 23.3 | −44.0 ± 6.9 | 86.9 | 13.0 |
BSA 115.2 | 115.2 | −32.7 ± 6.3 | 96.7 | 3.2 |
CS 22.5 | 22.5 | 37.9 ± 13.0 | 67.3 | 32.6 |
CS 44.1 | 44.1 | 48.3 ± 7.9 | 97.5 | 2.4 |
CS 133.7 | 133.7 | 52.0 ± 5.4 | 95.9 | 4.1 |
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Martínez-Robles, Á.M.; Loyola-Rodríguez, J.P.; Zavala-Alonso, N.V.; Martinez-Martinez, R.E.; Ruiz, F.; Lara-Castro, R.H.; Donohué-Cornejo, A.; Reyes-López, S.Y.; Espinosa-Cristóbal, L.F. Antimicrobial Properties of Biofunctionalized Silver Nanoparticles on Clinical Isolates of Streptococcus mutans and Its Serotypes. Nanomaterials 2016, 6, 136. https://doi.org/10.3390/nano6070136
Martínez-Robles ÁM, Loyola-Rodríguez JP, Zavala-Alonso NV, Martinez-Martinez RE, Ruiz F, Lara-Castro RH, Donohué-Cornejo A, Reyes-López SY, Espinosa-Cristóbal LF. Antimicrobial Properties of Biofunctionalized Silver Nanoparticles on Clinical Isolates of Streptococcus mutans and Its Serotypes. Nanomaterials. 2016; 6(7):136. https://doi.org/10.3390/nano6070136
Chicago/Turabian StyleMartínez-Robles, Ángel Manuel, Juan Pablo Loyola-Rodríguez, Norma Verónica Zavala-Alonso, Rita Elizabeth Martinez-Martinez, Facundo Ruiz, René Homero Lara-Castro, Alejandro Donohué-Cornejo, Simón Yobanny Reyes-López, and León Francisco Espinosa-Cristóbal. 2016. "Antimicrobial Properties of Biofunctionalized Silver Nanoparticles on Clinical Isolates of Streptococcus mutans and Its Serotypes" Nanomaterials 6, no. 7: 136. https://doi.org/10.3390/nano6070136