Platinum Nanoparticles Decrease Reactive Oxygen Species and Modulate Gene Expression without Alteration of Immune Responses in THP-1 Monocytes
Abstract
:1. Introduction
2. Materials and Methods
2.1. Platinum Nanoparticles Synthesis and Characterization
2.2. Cell Culture
2.3. Transmission Electron Microscopy (TEM) Analysis of Cellular Internalization of PtNPs
2.4. Intracellular Uptake of Pt NPs by Flow Cytometry Analysis
2.5. Annexin-PI Assay
2.6. WST-8 Assay
2.7. Receptor Expression
2.8. Inflammatory Cytokine Release
2.9. DCFDH-DA Assay
2.10. RNA Extraction and Microarray Analysis
2.11. Statistical Analysis
3. Results
3.1. Cytocompatibility of PtNPs with THP-1 Monocytes
3.2. THP-1 Monocyte Internalization of PtNPs
3.3. Immune Receptor Expression in the Presence of PtNPs
3.4. Cytokine Expression in the Presence of PTNPs
3.5. Reactive Oxigen Species ( ROS) Scavenging Activity of PtNPs in THP-1 Monocytes
3.6. Gene Transcription in THP-1 Treated with PtNPs
3.7. Cytokine Expression in PtNP-Treated THP-1 after LPS Stimulation
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Acknowledgments
Conflicts of Interest
References
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Gatto, F.; Moglianetti, M.; Pompa, P.P.; Bardi, G. Platinum Nanoparticles Decrease Reactive Oxygen Species and Modulate Gene Expression without Alteration of Immune Responses in THP-1 Monocytes. Nanomaterials 2018, 8, 392. https://doi.org/10.3390/nano8060392
Gatto F, Moglianetti M, Pompa PP, Bardi G. Platinum Nanoparticles Decrease Reactive Oxygen Species and Modulate Gene Expression without Alteration of Immune Responses in THP-1 Monocytes. Nanomaterials. 2018; 8(6):392. https://doi.org/10.3390/nano8060392
Chicago/Turabian StyleGatto, Francesca, Mauro Moglianetti, Pier Paolo Pompa, and Giuseppe Bardi. 2018. "Platinum Nanoparticles Decrease Reactive Oxygen Species and Modulate Gene Expression without Alteration of Immune Responses in THP-1 Monocytes" Nanomaterials 8, no. 6: 392. https://doi.org/10.3390/nano8060392