Optical Biosensing System for the Detection of Survivin mRNA in Colorectal Cancer Cells Using a Graphene Oxide Carrier-Bound Oligonucleotide Molecular Beacon
Abstract
:1. Introduction
2. Results and Discussion
2.1. Hairpin Structures of SurMB-Joe, tDNA Target, and the Hybridization Product
2.2. Supramolecular Interactions of SurMB-Joe Components with GO Nanocarriers
2.3. Desorptive Hybridization of GO-Bound SurMB-Joe with Complementary tDNA Target and Mutants
2.4. Detection of Survivin mRNA in Colorectal Cancer Cells SW480 Using GO@SurMB-Joe Nanoprobes
2.5. Mechanism of Hairpin–Hairpin Interactions in Desorptive Hybridization of GO@SurMB-Joe and tDNA
3. Materials and Methods
3.1. Chemicals
3.2. Apparatus
3.3. Cell Culture
3.4. Cell Transfection
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Ratajczak, K.; Krazinski, B.E.; Kowalczyk, A.E.; Dworakowska, B.; Jakiela, S.; Stobiecka, M. Optical Biosensing System for the Detection of Survivin mRNA in Colorectal Cancer Cells Using a Graphene Oxide Carrier-Bound Oligonucleotide Molecular Beacon. Nanomaterials 2018, 8, 510. https://doi.org/10.3390/nano8070510
Ratajczak K, Krazinski BE, Kowalczyk AE, Dworakowska B, Jakiela S, Stobiecka M. Optical Biosensing System for the Detection of Survivin mRNA in Colorectal Cancer Cells Using a Graphene Oxide Carrier-Bound Oligonucleotide Molecular Beacon. Nanomaterials. 2018; 8(7):510. https://doi.org/10.3390/nano8070510
Chicago/Turabian StyleRatajczak, Katarzyna, Bartlomiej E. Krazinski, Anna E. Kowalczyk, Beata Dworakowska, Slawomir Jakiela, and Magdalena Stobiecka. 2018. "Optical Biosensing System for the Detection of Survivin mRNA in Colorectal Cancer Cells Using a Graphene Oxide Carrier-Bound Oligonucleotide Molecular Beacon" Nanomaterials 8, no. 7: 510. https://doi.org/10.3390/nano8070510