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Article

Exogenous Production of Silver Nanoparticles by Tephrosia apollinea Living Plants under Drought Stress and Their Antimicrobial Activities

by
Muna A. Ali
1,
Kareem A. Mosa
1,2,*,
Ali El-Keblawy
1 and
Hussain Alawadhi
3
1
Department of Applied Biology, College of Sciences, University of Sharjah, Sharjah P.O. Box 27272, UAE
2
Department of Biotechnology, Faculty of Agriculture, Al-Azhar University, Cairo 11651, Egypt
3
Center for Advanced Materials Research, Research Institute of Sciences and Engineering, University of Sharjah, Sharjah P.O. Box 27272, UAE
*
Author to whom correspondence should be addressed.
Nanomaterials 2019, 9(12), 1716; https://doi.org/10.3390/nano9121716
Submission received: 30 September 2019 / Revised: 11 November 2019 / Accepted: 26 November 2019 / Published: 1 December 2019
(This article belongs to the Special Issue Toxicological Impact of Nanomaterials on Plants)
Browse Figures
Versions Notes

Abstract

:
Nanoparticle (NP) synthesis by biological systems is more cost-effective, safe, and environmentally friendly when compared to currently used chemical and physical methods. Although many studies have utilized different plant extracts to synthesize NPs, few studies have incorporated living plants. In this study, silver nanoparticles (AgNPs) were synthesized exogenously by Tephrosia apollinea living plant system under the combined stresses of silver nitrate and different levels of drought stress simulated by Polyethylene glycol (PEG) (0, −0.1, −0.2, and −0.4 MPa for three and six days). Biomass, cell death, and H2O2 content were evaluated to determine the toxicological effect of the treatments on the plant. More severe effects were detected in day 6 plants compared to day 3 plants, and at higher drought levels. UV-visible spectrum, energy dispersive X-ray spectroscopy, X-ray diffraction, scanning electron microscope, and Fourier transform infrared spectroscopy were used to detect and characterize the T. apollinea synthesized NPs. The shapes of the NPs were spherical and cubic with different phytochemicals being the possible capping agents. Broth microdilution was used to determine the antimicrobial activity of the NPs against Escherichia coli and Staphylococcus aureus. In this case, antimicrobial activity increased at higher PEG concentrations. Bactericidal effects were observed against E. coli, while only bacteriostatic effects were detected against S. aureus.

Graphical Abstract

1. Introduction

Nanoparticles (NPs) are used in various applications, including environmental, medical, nanobiosensors, agriculture, and clinical clothing [1]. Among the various types of NPs, silver nanoparticles (AgNPs) are one of the most commercialized, comprising over 50% of global nanomaterial-based market products available in 2015 [2]. AgNPs are used widely due to their unique antimicrobial, optical, electromagnetic, and physicochemical properties [3]. The synthesis of NPs is dependent upon chemical- or physical-based methods that are expensive and require harmful chemicals despite their efficiency [4]. In addition, these methodologies require harsh environments for the synthesis reaction to occur, including extremes of temperature, pressure, and pH [5].
Recently, biological methods were utilized as a green method for NP synthesis, involving the use of biological systems like bacteria, fungi, yeast, algae, and plants [6]. These methods are considered more cost-effective, safe, and eco-friendly [7]. In addition, they require ambient conditions like room temperature, atmospheric pressure, and physiological pH [8]. Among biological systems, living plants are renewable, safe to handle and easily available resources for nanoparticle production [1]. In contrast to bacteria, plant systems eliminate the need to frequently maintain cell cultures [8]. Extracts of different plant species have been utilized to synthesize NPs as they comprise different biomolecules that act as reducing agents [6]. AgNPs synthesized by plant extracts have a variety of applications in the medical field. Examples of plant extracts utilized to synthesize AgNPs with antimicrobial activity include Cressa cretica [9] and Azadirachta indica [10]. Anticancer activity was found in AgNPs synthesized using the extracts of Cyperus conglomeratus [11] and Syzygium aromaticum [12]. In addition, extracts of Tephrosia tinctoria [13] and Argyreia nervosa [14] demonstrated promising antidiabetic activities.
Although plant extracts have been widely used for nanoparticle synthesis [15], only a few studies have investigated the synthesis of AgNPs using living plants. In comparison to the production of NPs using plant extracts, living plants can reduce the spillover of toxic chemicals into the environment [3]. Thus, living plants are promising resources for nanoparticle synthesis that can meet the growing market demand for nanoparticle production. The synthesis of NPs depends on reducing agents and phytochemicals which play an important role in the reduction of Ag+ to Ag0 and the subsequent stabilization of the formed NPs [7]. Different plant species release different groups of phytochemicals. It has been reported that Vigna radiata plants treated with AgNO3 exhibited reduced levels of proteins and sugars, suggesting their probable role in NP synthesis [3].
Tephrosia apollinea (Delile) DC. is a perennial shrub native to the United Arab Emirates that grows in the lower mountain regions [16]. It has been used as a traditional medicine to relieve nasal congestion, earache, wounds, and bone fractures [17]. In addition, the plant has demonstrated insecticidal, anticancer and antibacterial properties [18]. In this regard, antibacterial compounds like tephrosin, deguelin, and quercetin were isolated from different Tephrosia spp. [17]. Furthermore, extracts of Tephrosia spp. were used for the synthesis of metal NPs. The stem extract of Tephrosia tinctoria was also utilized to synthesize AgNPs with promising antidiabetic properties [13]. In addition, AgNPs were synthesized from the leaf extract of Tephrosia purpurea and found to possess antimicrobial properties [19]. Antibacterial gold NPs were also synthesized using the leaf extract of T. purpurea [20].
In this study, the ability of T. apollinea to exogenously synthesize AgNPs is reported under the combined stress of silver ions and different levels of drought based on plant treatments. Furthermore, the antimicrobial activity of the isolated AgNPs was investigated. To the best of the authors’ knowledge, this is the first report to investigate the antimicrobial activities of NPs synthesized by living plant roots, as well as the first time that living T. apollinea plants were employed for the biosynthesis of NPs.

2. Materials and Methods

2.1. Seed Germination

Seeds of T. apollinea collected from Wadi Al-Ejili, Ras Al-Khaima in the United Arab Emirates were immersed in concentrated sulfuric acid for 25 min with stirring. Subsequently, they were washed several times with running tap water and then with distilled water. Seeds were then soaked in distilled water and incubated in a growth chamber adjusted at 30 °C/20 °C (12 h day/12 h night) at medium light intensity for 24 h. Accordingly, the seeds were germinated on filter papers in petri plates with 5 mL of distilled water and incubated until radical emergence. Seedlings were then transferred to strainers in 100 mL beakers filled with distilled water. Five plants were grown in each beaker. They were incubated at 30 °C/20 °C (12 h day/12 h night) at medium light intensity. After one week, the distilled water was replaced with 15% Hoagland’s solution with a final pH of 5.7–5.8. During incubation, Hoagland’s solution was added as needed. After seven weeks, plants were treated with solutions as explained in the subsequent section.

2.2. Preparation of Treatment Solutions and Plant Harvest

T. apollinea plants were treated with 15% Hoagland’s media with 1 mM AgNO3 alone or with different PEG concentrations corresponding to −0.1 MPa, −0.2 MPa, and −0.4 MPa. The control solutions contained only 15% Hoagland’s media. These PEG levels were selected based on a preliminary experiment tested the tolerance of T. apollinea to osmotic stress. The final pH of all the solutions was adjusted to 5.7–5.8. Eight replicas were prepared for each treatment, and the plants were incubated in the same conditions described above. The day of adding the treatment solutions was considered as day 0. Four plant replicas of each treatment were harvested on day 3, and the other four replicas were harvested on day 6. Plants were harvested by separating the shoots and roots, and the fresh biomass was measured. The plant tissues were stored at −80 °C until being ground in liquid nitrogen to form a fine powder which was stored at −80 °C for further analysis. The electrical conductivity (EC) of the growth media solutions were measured at day 0, day 3, and day 6 respectively. After plant harvest, the solutions were kept in the dark at room temperature.

2.3. Evan’s Blue Staining

The control and treated plant root tips were stained with 0.25% (w/v) of Evan’s blue (Himedia) in 0.1 M CaCl2 for 15 min with gentle shaking at room temperature. Subsequently, roots were washed three times with CaCl2 followed by three further washings with distilled water and observed using a bright-field microscope (Optika B-1000 BF, Ponteranica, Italy).

2.4. Measurement of H2O2 Content

H2O2 content was measured as described previously [21] with slight modifications. 100 mg of frozen roots were homogenized with 1 mL of 0.1% trichloroacetic acid (TCA) solution and then centrifuged at 13,000× g for 10 min at 4 °C. The clear supernatant (50 µL) was mixed with 50 µL of 10 mM potassium phosphate buffer (pH 7) and 100 µL of 1 M potassium iodide, with the absorbance measured at 390 nm (Epoch™ 2 Microplate Spectrophotometer, BioTek Instruments, Inc., Winooski, VT, USA). The H2O2 content was determined using an extinction coefficient of 0.28 µM−1 cm−1.

2.5. Nanoparticle Characterization

The solutions obtained from the replicas of each treatment were combined and centrifuged at 5500 RPM for 20 min; the supernatant was discarded, and the pellet was washed once with deionized water and air-dried. The pellets were analyzed by XRD (D8 Advance, Bruker AXS, Karlsruhe, Germany) with step size 0.03°, time/step = 0.50 s, and Cu kα as the X-ray source. In addition, the pellets were analyzed by FT-IR (FT/IR 6300, Jasco, Tokyo, Japan). 1 mg of the pellet was dispersed in 500 µL deionized water and characterized with UV-vis spectrophotometry (Epoch™ 2 Microplate Spectrophotometer, BioTek Instruments, Inc., Winooski, VT, USA), with the wavelength range between 300 nm and 700 nm. The prepared nanoparticle suspensions were sonicated for 30 min (XUBA Analogue Ultrasonic Bath, Grant instruments, Cambridgeshire, United Kingdom). 10 µL of the sonicated suspension was placed on the aluminum slide and left to air dry. SEM and EDS analyses were carried out using a TESCAN VEGA3 XM SEM, SE Detector, 30 kV, high vacuum (TESCAN, Brno – Kohoutovice, Czech Republic).

2.6. Antimicrobial Broth Microdilution Assay

The synthesized AgNPs were tested for their antimicrobial activities against E. coli (ATCC 25922) and S. aureus (ATCC 29213). The minimum inhibitory concentration (MIC) was determined using a broth microdilution assay prepared according to the Clinical and Laboratory Standards Institute (CLSI) [22,23]. Briefly, 100 µL of 2000 µg/mL nanoparticle suspension was added to the first column of a 96-well plate and 1:2 dilution series with Mueller Hinton Broth (MHB) was performed. Then, overnight cultures of E. coli and S. aureus grown on nutrient agar at 37 °C were diluted with sterile normal saline to OD600 of 0.1, resulting in a suspension of 1–2 × 108 colony forming units (CFUs) mL−1. Subsequently, the bacterial suspension was diluted by 1:10 and 5 µL of the diluted suspension was added to each well. In each plate, a row containing only MHB and a column containing bacterial suspension in the absence of NPs served as negative and positive controls respectively. Each treatment was tested in triplicates. The plate was incubated in a shaker at 37 °C overnight and bacterial growth was observed by visual inspection. The MIC was considered as the lowest concentration of NPs that inhibited the visible growth of bacteria. To determine the minimum bactericidal concentration (MBC), an aliquot of 3 µL obtained from all wells showing no visible bacterial growth was spotted onto nutrient agar plates and incubated overnight at 37 °C. MBC was defined as the lowest concentration that completely inhibited bacterial growth on the plate [24]. The broth microdilution experiment was repeated three times and the obtained MIC and MBC values were representative of the typical results.

2.7. Statistical Analysis

The student’s t-test was used to assess the significant difference between the mean values obtained from treated and control plants respectively. A p-value < 0.05 was considered as statistically significant. The student’s t-test was done using R [25]. The obtained data were categorized based on the p-value and indicated with *: p ≤ 0.05, and **: p ≤ 0.01. R packages of ggplot2 [26] and dplyr [27] were used to create certain graphs. Error bars in the figures represent the standard error.

3. Results and Discussion

3.1. Effects of AgNO3 and PEG on Plant Phenotype and Biomass

Control plants featured green healthy leaves on both days 3 and 6. The phenotype of the plant leaves treated with 1 mM AgNO3 were similar to control plants on day 3 but demonstrated chlorosis and started to wilt by day 6. Plants treated with 1 mM AgNO3 and PEG wilted and demonstrated yellowish leaves, with the severity of symptoms dependent on increased PEG concentrations as well as increased incubation times Figure 1a. On day 0, all the solution colors were transparent. However, on day 3 and day 6, the solution colors of treated plants turned brown, with darker colors observed in plants treated with PEG of −0.4 MPa. The alteration of media color from transparent to brown or dark brown indicates the formation of AgNPs [5,28]. The variation in solution colors might be due to differences in sizes and shapes of the formed NPs [29].
Fresh biomass was measured to evaluate the effect of AgNO3 and PEG treatments on T. apollinea growth Figure 1b. T. apollinea plants treated with 1 mM AgNO3 showed similar biomass as control plants on both day 3 and day 6. On the other hand, plants treated with different concentrations of PEG had reduced biomass in comparison to control plants. Increased plant biomass reduction was observed to be in accordance with increased PEG concentration. Biomass reduction under drought stress is commonly observed among plants [30]. Additionally, Cassia angustifolia plants treated with different concentrations of PEG also revealed reduced biomass, with more severe symptoms demonstrated at higher PEG concentrations [31]. A similar result was obtained in investigations where Sesuvium portulacastrum was treated with PEG [32]. Treatment with AgNO3 also led to a significant reduction in the biomass of Spirodela polyrhiza [33] and Brassica sp. [34] respectively.

3.2. AgNO3 and PEG Exhibited Increase in T. apollinea EC

For all treated plants, the EC of the media increased on days 3 and 6 respectively compared to day 0, as shown in Figure 2. This increase in EC may be attributed to the attractive interactions between AgNPs and silver ions, suggesting increased AgNP numbers in the media [35,36]. EC was also observed to reduce under increasing PEG concentrations. This may be due to the presence of more unreacted silver ions at lower PEG concentrations [37]. In addition, on days 0, 3, and 6, the EC of control plant solutions was lower in comparison to treated plants, except at 0.4 PEG on day 6 which was similar to the control EC value. The lower EC values at higher PEG concentrations may be related to the interactions of the ions with PEG [38].

3.3. AgNO3 and PEG Stresses Reduced Viability of T. apollinea Root Cells

Root tips were stained with Evan’s blue to detect cell membrane damage, which reflects cell death. In this case, viable cells exclude while dead cells retain Evan’s blue stain. Plants treated with either AgNO3 alone or different concentrations of PEG showed increased uptake of Evan’s blue stain (indicated by the intense blue color obtained) demonstrating reduced cell viability Figure 3. Cell death is caused by different types of abiotic stresses, including drought and metals. As such, exposure of Sorghum bicolor to drought stress resulted in reduced cell viability [39]. Furthermore, the treatment of Pandanus odorifer with silver ions or AgNPs caused root cell death [40]. Similarly, Coriandrum sativum plants treated with different concentrations of copper NPs also revealed root cell death [41].

3.4. AgNO3 and PEG Exposure Caused Oxidative Stress in T. apollinea Roots

H2O2 is one of the reactive oxygen species (ROS) produced under drought stress [42]. It causes many deleterious effects to plants including lipid peroxidation, disruption of cellular metabolic function as well as affecting cellular integrity [43]. H2O2 levels in T. apollinea roots were increased significantly in treated plants Figure 4. This indicates that the treatments caused oxidative stress in T. apollinea roots. A similar pattern was observed in Coriandrum sativum plants treated with copper NPs, where only the roots were oxidatively stressed [41]. Exposure of Cucumis sativus plants to copper NPs also leads to a significant H2O2 increase in roots [44].

3.5. Detection of Exogenous AgNPs Synthesised by T. apollinea Plants

The formation of exogenous AgNPs was detected by the Uv-vis absorption spectra of treatment suspensions. Broad surface plasmon resonance (SPR) peaks between 370–430 nm were observed on both day 3 and day 6, as shown in Figure 5a. The SPR spectra depends on the size, morphology, and composition of the NPs [45]. According to previous studies, absorption at this range is due to the SPR of AgNPs [45,46]. In addition, the position of the absorption peak varies depending on plant species [28].
SEM results showed the presence of NPs in the prepared colloidal solutions of treated plants on both days 3 and 6 Figure 5b. Most of the formed NPs were round, with some cubic NPs also observed. EDS and element mapping results indicated the presence of silver in the NPs Figure 5c. The EDS peaks around 3 KeV indicates the presence of silver [47,48]. In addition, the resulting peaks of carbon and oxygen were possibly due to organic phytochemicals that acted as capping agents of the synthesized NPs. This was observed in silver nanoparticles synthesized by the extract of Kappaphycus alverazii [49] and the cell-free filtrate of Aspergillus flavus NJP08 [50]. The additional peak of aluminium was due to the aluminium slide used in EDS.

3.6. XRD Analysis of the Synthesized Nanoparticles

The crystalline nature of the synthesized NPs was confirmed using XRD Figure 6. In all treatments, peaks were found at 2θ values of 38°, 46°, 67°, and 76°, which correspond to the (111), (200), (220), and (311) planes of the face-centered cubic (fcc) silver crystal, respectively (JCPDS no. 00-04-0783) [51]. In addition, there are other peaks that may correspond to silver oxide nanoparticles (JCPDS no. 00-076-1393) [52], which could be resulted from the oxidative leaching of Ag+ from already formed AgNPs. This was observed in the green synthesis of AgNPs by 16 different species of living plant systems [28]. Moreover, it was found in AgNPs developed from purple acid phosphatase apoenzyme isolated from Limonia acidissima [53].

3.7. Encapsulation of AgNPs and T. apollinea Phytochemicals

FT-IR analysis was performed to identify possible interactions between synthesized AgNPs and plant bioactive molecules that may act as capping and stabilizing agents, as shown in Figure 7. The band around 3300 cm−1 could be due to N–H and O–H stretching [53]. The band around 1630 cm−1 might indicate C=O of amide I bond, and the bands at 1385 and 1030 cm−1 could match with C–N stretching vibrations of the aromatic and aliphatic amines, respectively [54]. The results indicate the binding of proteins serving as capping agents with the synthesized silver nanoparticles. A similar result was obtained previously upon the synthesis of extracellular AgNPs by Aspergillus flavus NJP08 [50]. The band around 2920 cm−1 could be attributed to O–H stretch of carboxylic acids, which was detected in AgNPs produced by the extract of Tephrosia tinctoria plant [13].

3.8. Phytosenthesized AgNP Produced by T. apollinea Exhibited Antimicrobial Activities

The synthesized NPs demonstrated antimicrobial activities against both E. coli and S. aureus. The NPs with the highest antimicrobial activity were synthesized at PEG treatment corresponding to −0.2 MPa day 3 and −0.4 MPa day 3 and day 6, with MIC values of 31.25 μg/mL against E. coli and 15.63 μg/mL against S. aureus Table 1. This might be a result of more NPs and less unreacted Ag+ ions present at higher PEG concentrations, which was concluded based on the EC results. The control plants’ media solution demonstrated no antimicrobial activity against both E. coli and S. aureus.
The antimicrobial activity of AgNPs was tested widely against several pathogenic bacteria. It has been established that AgNPs possess strong antimicrobial activity against both Gram-positive and Gram-negative bacteria [55]. However, previous investigations have not provided a definite answer on whether Gram-negative [56] or Gram-positive bacteria [57,58] are more sensitive to AgNPs. While most of the T. apollinea synthesized NPs in our study were bactericidal against E. coli, they were only bacteriostatic against S. aureus. The higher sensitivity of Gram-negative E. coli to AgNPs compared to gram-positive S. aureus is due to the structural features of the bacterial cell wall. The cell wall of Gram-positive bacteria (30 nm) is thicker than Gram-negative bacteria (5–6 nm) due to the presence of multiple layers of peptidoglycan in the cell wall of Gram-Positive bacteria [56]. As a result, gram-negative bacteria may indeed be more susceptible to AgNPs [24].

4. Conclusions

AgNPs were successfully synthesized exogenously by T. apollinea plants under the combined stress of silver ions and drought on days 3 and 6 respectively. More severe effects on plants were observed at higher PEG levels in relation to biomass and root cell death. Treatments also caused oxidative damage in roots. Most of the characterization results were similar in both NPs isolated after days 3 or 6. The shapes of the NPs were spherical and cubic with different phytochemicals being the possible capping agents involved. The EC values were decreased at higher PEG concentrations, indicating the presence of lower ionic silver. This last factor may explain the reason behind the higher antimicrobial activity of NPs synthesized at PEG treatment corresponding to −0.4 MPa against E. coli and S. aureus respectively. To the best of the authors’ knowledge, the findings of this manuscript presented for the first time the antimicrobial activity of AgNPs synthesized via living plants. Further studies are required to investigate the other possible applications of such living plant-derived NPs. In addition, more plant stress parameters should be evaluated along with the ability of plants to recover following treatment. It is our hope that such investigations will eventually lead to the possibility of utilizing plants as bio-factories for the sustainable synthesis of NPs.

Author Contributions

Conceptualization, K.A.M.; methodology, K.A.M. and M.A.A.; validation, K.A.M., M.A.A., and A.E.-K.; formal analysis, K.A.M., M.A.A., H.A.; investigation, K.A.M., M.A.A., A.E.-K. and H.A.; resources, K.A.M.; data curation, K.A.M., M.A.A., A.E.-K. and H.A.; writing—original draft preparation, M.A.A.; writing—review and editing, K.A.M., A.E.-K.; supervision, K.A.M., A.E.-K., funding acquisition, K.A.M.

Funding

This research was funded by the Office of VC for Research and Graduate Studies at the University of Sharjah.

Acknowledgments

We would like to thank the following people from the University of Sharjah, UAE; Ahmed Ali for his kind help with thoughtful discussion and useful comments, Mohamed Shameer, and Muhammad Adil Abbasi for their help on NP characterization, and Islam M. Ahmady for her help on antimicrobial analysis.

Conflicts of Interest

The authors declare no conflict of interest.

References

  1. Prasad, R. Synthesis of silver nanoparticles in photosynthetic plants. J. Nanopart. 2014, 2014, 963961. [Google Scholar] [CrossRef]
  2. Inshakova, E.; Inshakov, O. World market for nanomaterials: Structure and trends. EDP Sci. 2017, 129, 02013. [Google Scholar] [CrossRef]
  3. Kumari, R.; Singh, J.S.; Singh, D.P. Biogenic synthesis and spatial distribution of silver nanoparticles in the legume mungbean plant (Vigna radiata L.). Plant Physiol. Biochem. 2017, 110, 158–166. [Google Scholar] [CrossRef]
  4. Kharissova, O.V.; Dias, H.R.; Kharisov, B.I.; Pérez, B.O.; Pérez, V.M.J. The greener synthesis of nanoparticles. Trends Biotechnol. 2013, 31, 240–248. [Google Scholar] [CrossRef]
  5. Raju, D.; Paneliya, N.; Mehta, U. Extracellular synthesis of silver nanoparticles using living peanut seedling. Appl. Nanosci. 2014, 4, 875–879. [Google Scholar] [CrossRef]
  6. Marchiol, L. Synthesis of metal nanoparticles in living plants. Ital. J. Agron. 2012, 7, e37. [Google Scholar] [CrossRef]
  7. Makarov, V.V.; Love, A.J.; Sinitsyna, O.V.; Makarova, S.S.; Yaminsky, I.V.; Taliansky, M.E.; Kalinina, N.O. “Green” nanotechnologies: Synthesis of metal nanoparticles using plants. Acta Nat. 2014, 6, 35–44. [Google Scholar] [CrossRef]
  8. Raju, D.; Mehta, U.J.; Ahmad, A. Phytosynthesis of intracellular and extracellular gold nanoparticles by living peanut plant (Arachis hypogaea L.). Biotechnol. Appl. Biochem. 2012, 59, 471–478. [Google Scholar] [CrossRef]
  9. Vijayalakshmi, M.; Akilandeswari, K.; Kavitha, K.; Gokila, S.; Vinothkumar, R. Green synthesis and antibacterial activity of silver nanoparticle using Cressa cretica plant extract. Chem. Lett. 2018, 1, 12–18. [Google Scholar]
  10. Ahmed, S.; Saifullah; Ahmad, M.; Swami, B.L.; Ikram, S. Green synthesis of silver nanoparticles using Azadirachta indica aqueous leaf extract. J. Radiat. Res. Appl. Sci. 2016, 9, 1–7. [Google Scholar] [CrossRef]
  11. Al-Nuairi, A.G.; Mosa, K.A.; Mohammad, M.G.; El-Keblawy, A.; Soliman, S.; Alawadhi, H. Biosynthesis, Characterization, and Evaluation of the Cytotoxic Effects of Biologically Synthesized Silver Nanoparticles from Cyperus conglomeratus Root Extracts on Breast Cancer Cell Line MCF-7. Biol. Trace Elem. Res. 2019, 1–10. [Google Scholar] [CrossRef]
  12. Venugopal, K.; Rather, H.A.; Rajagopal, K.; Shanthi, M.P.; Sheriff, K.; Illiyas, M.; Rather, R.A.; Manikandan, E.; Uvarajan, S.; Bhaskar, M. Synthesis of silver nanoparticles (Ag NPs) for anticancer activities (MCF 7 breast and A549 lung cell lines) of the crude extract of Syzygium aromaticum. J. Photochem. Photobiol. B Biol. 2017, 167, 282–289. [Google Scholar] [CrossRef]
  13. Rajaram, K.; Aiswarya, D.C.; Sureshkumar, P. Green synthesis of silver nanoparticle using Tephrosia tinctoria and its antidiabetic activity. Mater. Lett. 2015, 138, 251–254. [Google Scholar] [CrossRef]
  14. Saratale, G.D.; Saratale, R.G.; Benelli, G.; Kumar, G.; Pugazhendhi, A.; Kim, D.S.; Shin, H.S. Anti-diabetic potential of silver nanoparticles synthesized with Argyreia nervosa leaf extract high synergistic antibacterial activity with standard antibiotics against foodborne bacteria. J. Clust. Sci. 2017, 28, 1709–1727. [Google Scholar] [CrossRef]
  15. Jemal, K.; Sandeep, B.V.; Pola, S. Synthesis, Characterization, and Evaluation of the Antibacterial Activity of Allophylus serratus Leaf and Leaf Derived Callus Extracts Mediated Silver Nanoparticles. J. Nanomater. 2017, 2017, 4213275. [Google Scholar] [CrossRef]
  16. Jongbloed, M. The Comprehensive Guide to the Wild Flowers of the United Arab Emirates; E.R.W.D.A.: Abu Dhabi, UAE, 2003. [Google Scholar]
  17. Ghazanfar, S.A.; Al-Sabahi, A.M.A. Medicinal Plants of Northern and Central Oman (Arabia). Econ. Bot. 1993, 47, 89–98. [Google Scholar] [CrossRef]
  18. Cheruth, A.J.; Al Baloushi, S.A.M.; Karthishwaran, K.; Maqsood, S.; Kurup, S.S.; Sakkir, S. Medicinally active principles analysis of Tephrosia apollinea (Delile) DC. growing in the UAE. BMC Res. Notes 2017, 10, 61. [Google Scholar] [CrossRef]
  19. Ajitha, B.; Reddy, Y.A.K.; Reddy, P.S. Biogenic nano-scale silver particles by Tephrosia purpurea leaf extract and their inborn antimicrobial activity. Spectrochim. Acta A Mol. Biomol. Spectrosc. 2014, 121, 164–172. [Google Scholar] [CrossRef]
  20. Jisha, E.R.; Balamurugan, G.; Edison, N.; Selvakumar, P.; Rathiga, R. Biogenic synthesis of Gold Nanoparticles using leaf extract of Tephrosia purpurea and study of their antibacterial effect. Int. J. Pharm Tech Res. 2012, 4, 1323–1331. [Google Scholar]
  21. Velikova, V.; Yordanov, I.; Edreva, A. Oxidative stress and some antioxidant systems in acid rain-treated bean plants: Protective role of exogenous polyamines. Plant Sci. 2000, 151, 59–66. [Google Scholar] [CrossRef]
  22. Mauro, N.; Schillaci, D.; Varvarà, P.; Cusimano, M.G.; Geraci, D.M.; Giuffrè, M.; Cavallaro, G.; Maida, C.M.; Giammona, G. Branched High Molecular Weight Glycopolypeptide With Broad-Spectrum Antimicrobial Activity for the Treatment of Biofilm Related Infections. ACS Appl. Mater. Interfaces 2018, 10, 318–331. [Google Scholar] [CrossRef] [PubMed]
  23. Ahmady, I.M.; Hameed, M.K.; Almehdi, A.M.; Arooj, M.; Workie, B.; Sahle-Demessie, E.; Han, C.; Mohamed, A.A. Green and Cytocompatible Carboxyl Modified Gold-Lysozyme Nanoantibacterial for Combating Multidrug-Resistant Superbugs. Biomater. Sci. 2019, 7, 5016–5026. [Google Scholar] [CrossRef] [PubMed]
  24. Erjaee, H.; Rajaian, H.; Nazifi, S. Synthesis and characterization of novel silver nanoparticles using Chamaemelum nobile extract for antibacterial application. Adv. Nat. Sci. Nanosci. Nanotechnol. 2017, 8, 25004. [Google Scholar] [CrossRef]
  25. R Core Team. R: A language and environment for statistical computing. In R Foundation for Statistical Computing; R Core Team: Vienna, Austria, 2018; Available online: https://www.R-project.org/ (accessed on 30 November 2019).
  26. Wickham, H. Ggplot2: Elegant Graphics for Data Analysis; Springer: New York, NY, USA, 2016. [Google Scholar]
  27. Wickham, H.; François, R.; Henry, L.; Müller, K. Dplyr: A Grammar of Data Manipulation. R Package Version 0.7.8. 2018. Available online: https://CRAN.R-project.org/package=dplyr (accessed on 30 November 2019).
  28. Pardha-Saradhi, P.; Yamal, G.; Peddisetty, T.; Sharmila, P.; Nagar, S.; Singh, J.; Nagarajan, R.; Rao, K.S. Reducing Strength Prevailing at Root Surface of Plants Promotes Reduction of Ag+ and Generation of Ag0/Ag2O Nanoparticles Exogenously in Aqueous Phase. PLoS ONE 2014, 9, e106715. [Google Scholar] [CrossRef] [PubMed]
  29. Rajasekharreddy, P.; Rani, P.U.; Sreedhar, B. Qualitative assessment of silver and gold nanoparticle synthesis in various plants: A photobiological approach. J. Nanopart. Res. 2010, 12, 1711–1721. [Google Scholar] [CrossRef]
  30. Jaleel, C.A.; Manivannan, P.; Wahid, A.; Farooq, M.; Al-Juburi, H.J.; Somasundaram, R.; Panneerselvam, R. Drought stress in plants: A review on morphological characteristics and pigments composition. Int. J. Agric. Biol. 2009, 11, 100–105. [Google Scholar]
  31. Shitole, S.M.; Dhumal, K.N. Effect of water stress by polyethylene glycol 6000 and sodium chloride on seed germination and seedling growth of Cassia angustifolia. Int. J. Pharm. Sci. Res. 2012, 3, 528. [Google Scholar]
  32. Slama, I.; Ghnaya, T.; Hessini, K.; Messedi, D.; Savouré, A.; Abdelly, C. Comparative study of the effects of mannitol and PEG osmotic stress on growth and solute accumulation in Sesuvium portulacastrum. Environ. Exp. Bot. 2007, 61, 10–17. [Google Scholar] [CrossRef]
  33. Jiang, H.; Li, M.; Chang, F.; Li, W.; Yin, L. Physiological analysis of silver nanoparticles and AgNO3 toxicity to Spirodela polyrhiza. Environ. Toxicol. Chem. 2012, 31, 1880–1886. [Google Scholar] [CrossRef]
  34. Vishwakarma, K.; Upadhyay, N.; Singh, J.; Liu, S.; Singh, V.P.; Prasad, S.M.; Chauhan, D.K.; Tripathi, D.K.; Sharma, S. Differential phytotoxic impact of plant mediated silver nanoparticles (AgNPs) and silver nitrate (AgNO3) on Brassica sp. Front. Plant Sci. 2017, 8, 1501. [Google Scholar] [CrossRef]
  35. Danwanichakul, P.; Suwatthanarak, T.; Suwanvisith, C.; Danwanichakul, D. The Role of Ammonia in Synthesis of Silver Nanoparticles in Skim Natural Rubber Latex. J. Nanosci. 2016, 2016, 1–6. [Google Scholar] [CrossRef]
  36. Suwatthanarak, T.; Than-ardna, B.; Danwanichakul, P.; Danwanichakul, D. Synthesis of silver nanoparticles in skim natural rubber latex at room temperature. Mater. Lett. 2016, 168, 31–35. [Google Scholar] [CrossRef]
  37. Tien, D.; Tseng, K.; Liao, C.; Huang, J.; Tsung, T. Discovery of ionic silver in silver nanoparticle suspension fabricated by arc discharge method. J. Alloys Compd. 2008, 463, 408–411. [Google Scholar] [CrossRef]
  38. Qiu, C.; Xiao, X.; Liu, R. Biomimetic synthesis of spherical nano-hydroxyapatite in the presence of polyethylene glycol. Ceram. Int. 2008, 34, 1747–1751. [Google Scholar] [CrossRef]
  39. Nxele, X.; Klein, A.; Ndimba, B.K. Drought and salinity stress alters ROS accumulation, water retention, and osmolyte content in sorghum plants. S. Afr. J. Bot. 2017, 108, 261–266. [Google Scholar] [CrossRef]
  40. Panda, B.B.; Panda, K.K.; Achary, V.M.M.; Krishnaveni, R.; Padhi, B.K.; Sarangi, S.N.; Sahu, S.N. In vitro biosynthesis and genotoxicity bioassay of silver nanoparticles using plants. Toxicol. Vitr. 2011, 25, 1097–1105. [Google Scholar] [CrossRef]
  41. AlQuraidi, A.O.; Mosa, K.A.; Ramamoorthy, K. Phytotoxic and Genotoxic Effects of Copper Nanoparticles in Coriander (Coriandrum sativum -Apiaceae). Plants 2019, 8, 19. [Google Scholar] [CrossRef] [Green Version]
  42. Zlatev, Z.; Lidon, F.; Ramalho, J.; Yordanov, I. Comparison of resistance to drought of three bean cultivars. Biol. Plant 2006, 50, 389–394. [Google Scholar] [CrossRef]
  43. Zlatev, Z.; Lidon, F.C. An overview on drought induced changes in plant growth, water relationsand photosynthesis. Emir. J. Food Agric. 2012, 24, 57–72. [Google Scholar]
  44. Mosa, K.A.; El-Naggar, M.; Ramamoorthy, K.; Alawadhi, H.; Elnaggar, A.; Wartanian, S.; Ibrahim, E.; Hani, H. Copper nanoparticles induced genotoxicty, oxidative stress, and changes in Superoxide Dismutase (SOD) gene expression in cucumber (Cucumis sativus) plants. Front. Plant Sci. 2018, 9, 872. [Google Scholar] [CrossRef] [Green Version]
  45. Shameli, K.; Ahmad, M.B.; Jazayeri, S.D.; Sedaghat, S.; Shabanzadeh, P.; Jahangirian, H.; Mahdavi, M.; Abdollahi, Y. Synthesis and Characterization of Polyethylene Glycol Mediated Silver Nanoparticles by the Green Method. Int. J. Mol. Sci. 2012, 13, 6639–6650. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  46. Yamal, G.; Sharmila, P.; Rao, K.S.; Pardha-Saradhi, P. Inbuilt Potential of YEM Medium and Its Constituents to Generate Ag/Ag2O Nanoparticles. PLoS ONE 2013, 8, e61750. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  47. Krishnaraj, C.; Jagan, E.G.; Rajasekar, S.; Selvakumar, P.; Kalaichelvan, P.T.; Mohan, N. Synthesis of silver nanoparticles using Acalypha indica leaf extracts and its antibacterial activity against water borne pathogens. Colloids Surf. B Biointerfaces 2010, 76, 50–56. [Google Scholar] [CrossRef] [PubMed]
  48. El-Naggar, N.E.; Hussein, M.H.; El-Sawah, A.A. Phycobiliprotein-mediated synthesis of biogenic silver nanoparticles, characterization, in vitro and in vivo assessment of anticancer activities. Sci. Rep. 2018, 8, 8925. [Google Scholar] [CrossRef]
  49. Ganesan, V.; Aruna Devi, J.; Astalakshmi, A.; Nima, P.; Thangaraja, A. Eco-friendly synthesis of silver nanoparticles using a sea weed, Kappaphycus alvarezii (Doty) Doty ex PC Silva. Int. J. Eng. Adv. Technol. 2013, 2, 559–563. [Google Scholar]
  50. Jain, N.; Bhargava, A.; Majumdar, S.; Tarafdar, J.C.; Panwar, J. Extracellular biosynthesis and characterization of silver nanoparticles using Aspergillus flavus NJP08: A mechanism perspective. Nanoscale 2011, 3, 635–641. [Google Scholar] [CrossRef]
  51. Salehi, S.; Shandiz, S.A.S.; Ghanbar, F.; Darvish, M.R.; Ardestani, M.S.; Mirzaie, A.; Jafari, M. Phytosynthesis of silver nanoparticles using Artemisia marschalliana Sprengel aerial part extract and assessment of their antioxidant, anticancer, and antibacterial properties. Int. J. Nanomed. 2016, 11, 1835–1846. [Google Scholar]
  52. Ravichandran, S.; Paluri, V.; Kumar, G.; Loganathan, K.; Kokati Venkata, B.R. A novel approach for the biosynthesis of silver oxide nanoparticles using aqueous leaf extract of Callistemon lanceolatus (Myrtaceae) and their therapeutic potential. J. Exp. Nanosci. 2016, 11, 445–458. [Google Scholar] [CrossRef] [Green Version]
  53. Pawar, O.; Deshpande, N.; Dagade, S.; Waghmode, S.; Nigam Joshi, P. Green synthesis of silver nanoparticles from purple acid phosphatase apoenzyme isolated from a new source Limonia acidissima. J. Exp. Nanosci. 2016, 11, 28–37. [Google Scholar] [CrossRef]
  54. Vigneshwaran, N.; Kathe, A.A.; Varadarajan, P.V.; Nachane, R.P.; Balasubramanya, R.H. Silver−Protein (Core−Shell) Nanoparticle Production Using Spent Mushroom Substrate. Langmuir ACS J. Surf. Colloids 2007, 23, 7113–7117. [Google Scholar] [CrossRef]
  55. Bedlovičová, Z.; Salayová, A. Green-Synthesized Silver Nanoparticles and Their Potential for Antibacterial Applications. In Bacterial Pathogenesis and Antibacterial Control, 1st ed.; Kırmusaoğlu, S., Ed.; IntechOpen: London, UK, 2018; pp. 73–94. [Google Scholar]
  56. Yun’an Qing, L.C.; Li, R.; Liu, G.; Zhang, Y.; Tang, X.; Wang, J.; Liu, H.; Qin, Y. Potential antibacterial mechanism of silver nanoparticles and the optimization of orthopedic implants by advanced modification technologies. Int. J. Nanomed. 2018, 13, 3311–3327. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  57. El Kassas, H.Y.; Attia, A.A. Bactericidal Application and Cytotoxic Activity of Biosynthesized Silver Nanoparticles with an Extract of the Red Seaweed Pterocladiella capillacea on the HepG 2 Cell Line. Asian Pac. J. Cancer Prev. APJCP 2014, 15, 1299–1306. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  58. Parlinska-Wojtan, M.; Kus-Liskiewicz, M.; Depciuch, J.; Sadik, O. Green synthesis and antibacterial effects of aqueous colloidal solutions of silver nanoparticles using camomile terpenoids as a combined reducing and capping agent. Bioprocess Biosyst. Eng. 2016, 39, 1213–1223. [Google Scholar] [CrossRef] [PubMed] [Green Version]
Nanomaterials 09 01716 g001 550
Figure 1. The effect of AgNO3 and Polyethylene glycol (PEG) treatments on (a) plant phenotypes and (b) biomass of T. apollinea at day 3 and day 6 of treatment with 1 mM AgNO3 alone or with different concentrations of PEG corresponding to −0.1, −0.2, and −0.4 MPa.
Figure 1. The effect of AgNO3 and Polyethylene glycol (PEG) treatments on (a) plant phenotypes and (b) biomass of T. apollinea at day 3 and day 6 of treatment with 1 mM AgNO3 alone or with different concentrations of PEG corresponding to −0.1, −0.2, and −0.4 MPa.
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Figure 2. Electrical conductivity of the control and the treated plant media. The figure represents the EC values of the prepared solution on day 0, and the average electrical conductivity (EC) on days 3 and 6 respectively. This figure was created using R [25].
Figure 2. Electrical conductivity of the control and the treated plant media. The figure represents the EC values of the prepared solution on day 0, and the average electrical conductivity (EC) on days 3 and 6 respectively. This figure was created using R [25].
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Nanomaterials 09 01716 g003 550
Figure 3. Evan’s blue staining assay of T. apollinea roots treated with 1 mM AgNO3 alone or with different concentrations of PEG corresponding to −0.1, −0.2, and −0.4 MPa respectively.
Figure 3. Evan’s blue staining assay of T. apollinea roots treated with 1 mM AgNO3 alone or with different concentrations of PEG corresponding to −0.1, −0.2, and −0.4 MPa respectively.
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Nanomaterials 09 01716 g004 550
Figure 4. H2O2 contents in the roots of T. apollinea plants treated with 1 mM AgNO3 alone or with different concentrations of PEG corresponding to −0.1, −0.2, and −0.4 MPa. This figure was created using R [25].
Figure 4. H2O2 contents in the roots of T. apollinea plants treated with 1 mM AgNO3 alone or with different concentrations of PEG corresponding to −0.1, −0.2, and −0.4 MPa. This figure was created using R [25].
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Nanomaterials 09 01716 g005 550
Figure 5. Detection of phytosynthesized AgNPs by T. apollinea using (a) Uv-vis, (b) SEM, and (c) EDS. Plants were treated with 1 mM AgNO3 alone or with different concentrations of PEG corresponding to −0.1, −0.2, and −0.4 MPa.
Figure 5. Detection of phytosynthesized AgNPs by T. apollinea using (a) Uv-vis, (b) SEM, and (c) EDS. Plants were treated with 1 mM AgNO3 alone or with different concentrations of PEG corresponding to −0.1, −0.2, and −0.4 MPa.
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Figure 6. XRD patterns of the AgNPs synthesized exogenously by T. apollinea at day 3 and day 6. Plants were treated with 1 mM AgNO3 alone or with different concentrations of PEG corresponding to −0.1 MPa, −0.2 MPa, and −0.4 MPa. The XRD patterns showed diffraction peaks corresponding to the planes of Ag0 and Ag2O based on JCPDSV cards of Ag0 and Ag2O.
Figure 6. XRD patterns of the AgNPs synthesized exogenously by T. apollinea at day 3 and day 6. Plants were treated with 1 mM AgNO3 alone or with different concentrations of PEG corresponding to −0.1 MPa, −0.2 MPa, and −0.4 MPa. The XRD patterns showed diffraction peaks corresponding to the planes of Ag0 and Ag2O based on JCPDSV cards of Ag0 and Ag2O.
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Nanomaterials 09 01716 g007 550
Figure 7. FT-IR spectra of silver nanoparticles synthesized exogenously by T. apollinea on day 3 and day 6 respectively. Plants were treated with 1 mM AgNO3 alone or with different concentrations of PEG corresponding to −0.1, −0.2, and −0.4 MPa.
Figure 7. FT-IR spectra of silver nanoparticles synthesized exogenously by T. apollinea on day 3 and day 6 respectively. Plants were treated with 1 mM AgNO3 alone or with different concentrations of PEG corresponding to −0.1, −0.2, and −0.4 MPa.
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Table
Table 1. The antimicrobial effect of phytosynthesized AgNPs produced by T. apollinea plants treated with 1 mM AgNO3 alone or with different concentrations of PEG corresponding to −0.1, −0.2, and −0.4 MPa. The values of MIC and MBC (μg/mL) are presented for different types of nanoparticles and silver nitrate against E. coli and S. aureus respectively.
Table 1. The antimicrobial effect of phytosynthesized AgNPs produced by T. apollinea plants treated with 1 mM AgNO3 alone or with different concentrations of PEG corresponding to −0.1, −0.2, and −0.4 MPa. The values of MIC and MBC (μg/mL) are presented for different types of nanoparticles and silver nitrate against E. coli and S. aureus respectively.
TreatmentDay E. coliS. aureus
MICMBCMICMBC
1 mM AgNO33125100031.25>1000
612550062.5>1000
1 mM AgNO3+−0.1Osmotic Potential (MPa) PEG 362.525031.25>1000
612525062.5>1000
−0.2331.2512515.625>1000
662.550062.5>1000
−0.4331.2512515.625>1000
631.2512515.625>1000

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Ali, M.A.; Mosa, K.A.; El-Keblawy, A.; Alawadhi, H. Exogenous Production of Silver Nanoparticles by Tephrosia apollinea Living Plants under Drought Stress and Their Antimicrobial Activities. Nanomaterials 2019, 9, 1716. https://doi.org/10.3390/nano9121716

AMA Style

Ali MA, Mosa KA, El-Keblawy A, Alawadhi H. Exogenous Production of Silver Nanoparticles by Tephrosia apollinea Living Plants under Drought Stress and Their Antimicrobial Activities. Nanomaterials. 2019; 9(12):1716. https://doi.org/10.3390/nano9121716

Chicago/Turabian Style

Ali, Muna A., Kareem A. Mosa, Ali El-Keblawy, and Hussain Alawadhi. 2019. "Exogenous Production of Silver Nanoparticles by Tephrosia apollinea Living Plants under Drought Stress and Their Antimicrobial Activities" Nanomaterials 9, no. 12: 1716. https://doi.org/10.3390/nano9121716

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