Valorization of Potato Peel Waste as Natural Additive for Use in Meat Products

Comments

Response

1. Potato peels contain glycoalkaloids that may present as health risk for consumption - authors should include some discussion around this concern.

Text changes were made to paragraph 1 of section 2.1., as well as to the second paragraph of section 3.1.

2. For total phenolics and antioxidant activity tests, the ethanol extract was used. However, in Materials and Methods section, no description on the extraction was provided. This should be added.

The changes were carried out on 2.1. section, paragraph two

3. Section and sub-section heading numbers should be checked and corrected. For example, in section 2, the sub-heading is 4. In section 3, the sub-heading is 2.

The numbering of the indicated subsections was corrected

Line 21-22: The sentence seems to be not completed

Changes were made in the abstract section

Comments

Response

It is known that potato peels are known to contain steroidal alkaloids that may have implications for the health of consumers. Wouldn't it be more cautious to comment on the need to evaluate such components in extracts before concluding that:

The use of potato peel waste powder as a natural antioxidant for meat products is recommended"?

Dear reviewer, I appreciate the accurate observation. To answer your question, text changes were made to paragraph 1 of section 2.1., as well as to the second paragraph of section 3.1.

Comments

Response

Please rewrite the abstract presenting the values for the measured parameters which are significant for the study. You also should mention in the abstract that you measured the Total Polyphenolic Content (TPC).

Changes were made to the abstract

In the Materials and Methods section, you did not describe the extraction of the potato peel in order to have the extracts that you tested further.

In order to address the observation, changes were made to section 2.1. paragraph two of the manuscript

An aliquot of the ethanol extract (100 μL, 500, 250, 125 and 62.5μg/mL)

Text writing was modified

You did not mention anywhere in the text that you tested different concentrations of extract and why you chose these concentrations.

The wording of the text is modified and the use of the different concentrations of the extract is included in section 2.1. paragraph 2

Concentrations lower than 1000 ppm are commonly used, for example 500 ppm, because they are concentrations at which certain food additives are used.

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In the Results and Discussion section please discuss the results in function of the values that you obtained and presented in tables.

In this case, the results are presented based on the information we obtained; however, we do not include the values in the text since these are already presented in the table. It is recommended to insert the values described in the table in text, so as not to be repetitive

The results presented in Figure 1 would be better shown in Table format so we can actually see the values.

The recommendation is appreciated, the decision was made to keep the data in figure format given that the manuscript already has 4 tables

Figure 1A; are you sure that 62.5 μg/mL (I assume this is the potato peel extract concentration) you have approximately 75 mg GAE/g TPHC?

A correction was carried out in the value of the first bar corresponding to the concentration 62.5 (Fig. 1A), in addition, the units of the Y axes were corrected, changing mg/g to ug/mL (Fig 1A and 1B).  Likewise, corrections were made to the units on lines 211 and 212. We appreciate the observation

Line 209 which is the concentration dependence? Explain please, because for instance in Figure 1A you have almost the same concentration of TPHC for both concentrations of 125 and250 μg/mL; the same for Figure 1B and 1C.

The use of concentration dependence was eliminated, and it was indicated that the highest content or best effect, respectively, was found at the highest concentration.

In the case of the methodologies used for both the quantification of metabolites and antioxidant tests, these are considered oxide-reductive tests, however, an increase in concentration does not mean that this increase in the result obtained is double the value. In some cases, by increasing the concentration, there may come a point where the values remain constant, while in other cases, by increasing the concentration, the values may decrease (quadratic effect), that is, there may be pro-oxidant effects.