Ascorbic Acid Rejection Characteristics of Modified Platinum Electrodes: A Shelf Life Investigation
Abstract
:1. Introduction
2. Experimental Section
2.1. Reagents and Solutions
2.2. Sensor Manufacture
2.3. Storage Conditions
2.4. AA Calibrations
2.5. Instrumentation and Software
3. Results and Discussion
3.1. Shelf-Life Studies—Effect of Repeated AA Calibrations
Pt-PPD | Pt-Nafion® (5/2) | Pt-Nafion® (1/2)-PPD | Pt-Nafion® (2/1)-PPD | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
Day | Mean I (pA) | SEM | Stats | Mean I (pA) | SEM | Stats | Mean I (pA) | SEM | Stats | Mean I (pA) | SEM | Stats |
1 | 138 | 19 | 131 | 29 | −59 | 10 | 2 | 10 | ||||
3 | 147 | 10 | ns | 260 | 211 | ns | 141 | 107 | ns | 8 | 1 | ns |
7 | 150 | 53 | ns | 592 | 328 | ns | 2583 | 944 | | 473 | 156 | |
14 | 590 | 224 | ns | 1254 | 902 | ns | 2910 | 1000 | | 1328 | 408 | |
28 | 1847 | 684 | | 1598 | 1099 | ns | 3299 | 1049 | | 2231 | 594 | |
56 | 2707 | 1031 | | 3239 | 1175 | | 6539 | 1504 | | 4434 | 665 | |
3.2. Shelf-Life Studies—Effect of Single AA Calibration
3.3. Effect of Various Storage Conditions on Shelf Life of Pt-PPD Electrodes
4 °C | 4 °C /N2 Saturated PBS | 4 °C /N2 Saturated | −20 °C | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
Day | Mean I (pA) | SEM | Stats | Mean I (pA) | SEM | Stats | Mean I (pA) | SEM | Stats | Mean I (pA) | SEM | Stats |
1 | 19 | 4 | 18 | 7 | 251 | 27 | 139 | 23 | ||||
56 | 24 | 2 | ns | 1123 | 121 | | 295 | 105 | ns | 804 | 302 | |
168 | 3490 | 1276 | | 4057 | 122 | | 222 | 131 | ns | 1706 | 615 | |
4. Conclusions
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Wynne, A.M.; Finnerty, N.J. Ascorbic Acid Rejection Characteristics of Modified Platinum Electrodes: A Shelf Life Investigation. Chemosensors 2015, 3, 55-69. https://doi.org/10.3390/chemosensors3020055
Wynne AM, Finnerty NJ. Ascorbic Acid Rejection Characteristics of Modified Platinum Electrodes: A Shelf Life Investigation. Chemosensors. 2015; 3(2):55-69. https://doi.org/10.3390/chemosensors3020055
Chicago/Turabian StyleWynne, Andrea M., and Niall J. Finnerty. 2015. "Ascorbic Acid Rejection Characteristics of Modified Platinum Electrodes: A Shelf Life Investigation" Chemosensors 3, no. 2: 55-69. https://doi.org/10.3390/chemosensors3020055