Diagnostic Accuracy of Flow Cytometric DNA Index in Saudi Children with B Cell Acute Lymphoblastic Leukemia
Abstract
:1. Introduction
2. Materials and Methods
3. Results
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Parameter | ||
---|---|---|
Total sample size | 72 (100%) | |
Gender | Male | 43 (59.7%) |
Female | 29 (40.3%) | |
Age range Median +/− SD | 0–14 years 5.40 +/− 3.789 | |
Type of sample | Bone marrow | 67 (93.1%) |
Leukemic blood | 5 (6.9%) | |
Karyotype | Total | 47/72 (65.3%) |
Normal | 19 (26.4%) | |
Abnormal | 28 (38.9%) | |
Failed | 21 (29.2%) | |
No result (ONLY FISH) | 4 (5.6%) | |
FISH | Total | 66/72 (91.7%) |
Normal | 10 (13.8%) | |
Abnormal | 56 (77.8%) | |
Failed | - | |
No result (ONLY karyotype) | 6 (8.3%) |
Karyotype | n (%) 47 |
---|---|
Normal | 19 (40.4%) |
Pseudodiploid (Chromosomal rearrangement: BCR/ABL, 4 case; Deletion 9, 4 cases; TCF3/PBX1, 1 case; KMT2A/MLLT1, 1 case) | 10 (21.27%) |
Hyperdiploid A (47–50) | 2 (4.2%) |
Hyperdiploid B (51–68) | 15 (31.91%) |
Hypodiploid <46 | 1 (2.1%) |
FISH | n (%) (Total = 66) |
---|---|
Normal | 10 (15.2%) |
Hyperdiploid A (47–50) Pseudo hyperdiploid A | 3 (4.5%) 2 (3.03%) |
Hyperdiploid B (51–68) Pseudo hyperdiploid A | 22 (33.4%) 1 (1.5%) |
BCR/ABL | 3 (4.5%) |
MLL Rearrangement | 2 (3.03%) |
ETV6/RINX1 | 14 (21.2%) |
TCF3/PBX1 | 2 (3.03%) |
CDNK2A deletion | 6 (9.1%) |
RUNX1 amp | 1 (1.5%) |
Type of Analysis | DNA Index Result | Category of Case Based on DI | Category of Case Based on CG | Status |
---|---|---|---|---|
Automatic | 1.17/2.28 | Hyperdiploid B/Tetraploid | Hyperdiploid B | Match |
1.19 | Hyperdiploid B | Hyperdiploid B | ||
1.19 | Hyperdiploid B | Hyperdiploid B | ||
1.21 | Hyperdiploid B | Hyperdiploid B | ||
1.22 | Hyperdiploid B | Hyperdiploid B | ||
1.24 | Hyperdiploid B | Hyperdiploid B | ||
1.26 | Hyperdiploid B | Hyperdiploid B | ||
1.26 | Hyperdiploid B | Hyperdiploid B | ||
1.32/2.58 | Hyperdiploid B/Tetraploidy | Hyperdiploid B | ||
1.34 | Hyperdiploid B | Hyperdiploid B | ||
1.99 | Tetraploid | Diploid | ||
Manual | 1.13 | Hyperdiploid B | Hyperdiploid B | Match |
1.18 | Hyperdiploid B | Hyperdiploid A | No Match | |
1.11 | Hyperdiploid B | Hyperdiploid A | ||
1.11 | Hyperdiploid B | Hyperdiploid B | ||
1.94 | Tetraploid | Hyperdiploid A | ||
1.11 | Hyperdiploid B | Hyperdiploid A | ||
1.21 | Hyperdiploid B | Hyperdiploid B | Match | |
1.34 | Hyperdiploid B | Hyperdiploid B | ||
1.27 | Hyperdiploid B | Hyperdiploid B | ||
1.16 | Hyperdiploid B | Hyperdiploid B | ||
1.10 | Hyperdiploid B | Hyperdiploid B | ||
1.20 | Hyperdiploid B | Hyperdiploid B | ||
1.23 | Hyperdiploid B | Hyperdiploid B | ||
1.09 | Hyperdiploid A | Hyperdiploid A | ||
1.23 | Hyperdiploid B | Hyperdiploid B | ||
1.21 | Hyperdiploid B | Hyperdiploid B | ||
1.23 | Hyperdiploid B | Hyperdiploid B | ||
1.24 | Hyperdiploid B | Hyperdiploid B | ||
Automatic 42 CASES | 1 | Diploid 42 CASES | Diploid 42 CASES | Match 42 CASES |
Total | 71 | |||
The sample was highly aggregating 96% | 1 | Exclude | ||
Total | 72 |
Case Number | Test | ISCN | Category of Abnormalities | Number of Chromosomes Based on Cytogenetic Test | DNA Index by MOD Fit Software |
---|---|---|---|---|---|
1 | Karyotype | 47,XXY?c[20] | Hyper-diploid A +X | 47 | 1.18 |
FISH | nuc ish(PBX1,TCF3)x2[200], (D4Z1,D10Z1)x2[200], (D6Z1x2)[200], (ABL1,BCR)x2[200], (CDNK2Ax2)[200], (MLLx2)(5′sep 3′MLLx1)[200], (ETV6,RUNX1)x2[200], (TP53x2)[200] | MLL rearrangement | |||
2 | Karyotype | 47, XX,+21[4]/46, XX[16] | Hyper-diploid A +21 | 47 46 | 1.11 |
FISH | nuc ish(PBX1,TCF3)x2[200], (D4Z1,D10Z1)x2[200], (D6Z1x2)[200], (ABL1,BCR)x2[200], (CDNK2Ax2)[200], (MLLx2)[200], (ETV6x2,RUNX1x3)[170/200], (TP53x2)[200] | Hyper-diploid A | 47 | ||
3 | Karyotype | No result | _ | _ | 1.94 |
FISH | nuc ish(PBX1,E2A)x2[200], (D4Z1,D10Z1)x2[200], (D6Z1x3)[168/200], (ABL1,BCR)x2[200], (CDNK2Ax2)[200], (MLLx2) (5′ MLL sep 3′MLLx1)[180/200], (ETV6,RUNX1)x2[200], (TP53x2)[200] | Hyper-diploid A MLL rearrangement | 47 | ||
4 | Karyotype | 45,XX,t(9;22)(q34;q11.2),add(13)(p11.2),−20[9]/ 47, XX,t(9;22)(q34;q11.2),+der(22)t(9;22)(q34;q11.2)[6] | Hypodiploid Hyper-diploid A | 45 47 | 1.11 |
FISH | nuc ish(PBX1,TCF3)x2[200], (D4Z1,D10Z1)x2[200], (D6Z1x2)[200], (ABL1x3),(BCRx3), (ABL1con BCRx2)[158/200], (D9Z1,CDNK2A)x2[200], (MLLx2)[200],(ETV6,RUNX1)x2[200], (TP53x2)[200]/ (ABL1x4),(BCRx4), (ABL1con BCRx3)[42/200] | Hyper-diploid A BCR/ABL | 48 |
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Alatawi, D.; Bakhsh, I.; Tashkandi, S.; Alqarni, A.; Shaik, A.P.; Abudawood, M. Diagnostic Accuracy of Flow Cytometric DNA Index in Saudi Children with B Cell Acute Lymphoblastic Leukemia. Children 2023, 10, 1299. https://doi.org/10.3390/children10081299
Alatawi D, Bakhsh I, Tashkandi S, Alqarni A, Shaik AP, Abudawood M. Diagnostic Accuracy of Flow Cytometric DNA Index in Saudi Children with B Cell Acute Lymphoblastic Leukemia. Children. 2023; 10(8):1299. https://doi.org/10.3390/children10081299
Chicago/Turabian StyleAlatawi, Dalal, Ismail Bakhsh, Soha Tashkandi, Abdullah Alqarni, Abjal Pasha Shaik, and Manal Abudawood. 2023. "Diagnostic Accuracy of Flow Cytometric DNA Index in Saudi Children with B Cell Acute Lymphoblastic Leukemia" Children 10, no. 8: 1299. https://doi.org/10.3390/children10081299