Defective Leukocyte β2 Integrin Expression and Reactive Oxygen Species Production in Neonates
Abstract
:1. Introduction
2. Materials and Methods
2.1. Patients
2.2. Integrin Cell Surface Expression
2.3. Superoxide Radical Production
2.4. Statistical Analysis
3. Results
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Neonates—Day 0 | Neonates—Day 3 | Adults | p-Value (Neonates Day 0 vs. Day 3) | p-Value (Neonates Day 0 vs. Adults) | p-Value (Neonates Day 3 vs. Adults) | ||
---|---|---|---|---|---|---|---|
Neutrophils | CD11a | 96.9 ± 2.7 | 98.5 ± 1.3 | 99.9 ± 0.1 | <0.01 * | <0.01 * | <0.01 * |
CD11b | 94.6 ± 7.5 | 95.3 ± 7.6 | 99.3 ± 1.4 | 0.70 | 0.04 * | 0.08 | |
CD11c | 87.3 ± 7.0 | 92.1 ± 7.6 | 94.5 ± 4.7 | <0.01 * | <0.01 * | 0.31 | |
CD18 | 98.0 ± 2.7 | 98.6 ± 2.9 | 99.6 ± 1.1 | 0.37 | 0.05 | 0.25 | |
Monocytes | CD11a | 96.7 ± 4.0 | 97.3 ± 4.3 | 99.9 ± 0.1 | 0.55 | <0.01 * | 0.12 |
CD11b | 84.2 ± 10.9 | 86.0 ± 9.0 | 92.4 ± 6.5 | 0.45 | 0.02 * | 0.03 * | |
CD11c | 88.2 ± 8.9 | 89.7 ± 10.6 | 94.1 ± 3.4 | 0.52 | 0.03 * | 0.17 | |
CD18 | 97.5 ± 3.5 | 98.9 ± 2.5 | 99.9 ± 0.2 | 0.06 | 0.02 * | 0.18 | |
Lymphocytes | CD11a | 93.8 ± 7.0 | 93.1 ± 10.2 | 99.8 ± 0.3 | 0.74 | <0.01 * | 0.03 * |
CD11b | 25.5 ± 9.9 | 14.1 ± 6.6 | 33.6 ± 7.5 | <0.01 * | <0.01 * | <0.01 * | |
CD11c | 11.5 ± 6.0 | 4.8 ± 2.6 | 12.8 ± 5.5 | <0.01 * | 0.51 | <0.01 * | |
CD18 | 89.2 ± 12.3 | 90.7 ± 11.4 | 98.6 ± 4.6 | 0.60 | 0.01 * | 0.03 * |
Resting | After Zymosan (Z) | After Opsonized Zymosan (OZ) | After PMA | |
---|---|---|---|---|
Day 0 | 4.39 ± 4.00 | 18.42 ± 17.56 | 59.53 ± 27.77 | 72.99 ± 29.06 |
Day 3 | 2.28 ± 3.58 | 6.22 ± 10.97 | 47.80 ± 22.97 | 63.12 ± 30.27 |
Adults | 2.61 ± 1.73 | 48.78 ± 34.20 | 69.02 ± 43.11 | 52.21 ± 28.66 |
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Capolupo, I.; De Rose, D.U.; Pascone, R.; Danhaive, O.; Orzalesi, M. Defective Leukocyte β2 Integrin Expression and Reactive Oxygen Species Production in Neonates. Children 2022, 9, 494. https://doi.org/10.3390/children9040494
Capolupo I, De Rose DU, Pascone R, Danhaive O, Orzalesi M. Defective Leukocyte β2 Integrin Expression and Reactive Oxygen Species Production in Neonates. Children. 2022; 9(4):494. https://doi.org/10.3390/children9040494
Chicago/Turabian StyleCapolupo, Irma, Domenico Umberto De Rose, Roberto Pascone, Olivier Danhaive, and Marcello Orzalesi. 2022. "Defective Leukocyte β2 Integrin Expression and Reactive Oxygen Species Production in Neonates" Children 9, no. 4: 494. https://doi.org/10.3390/children9040494