The Evaluation of Magnetic Polymethacrylate-based Microspheres Used for Solid Phase DNA Micro-Extraction
Abstract
:1. Introduction
2. Materials
2.1. Chemicals and Equipment
Microsphere | Fe content (%) | -COOH (mM g −1) | Diameter (μm) | PDI * |
---|---|---|---|---|
P(HEMA-co-GMA) (A1) | 5.94 | 0.00 | 2.86 | 1.04 |
P(HEMA-co-GMA) (A2) | 5.94 | 0.41 | 2.86 | 1.04 |
PGMA (B1) | 5.95 | 0.00 | 0.74 | 1.07 |
PGMA (B2) | 5.95 | 0.42 | 0.74 | 1.07 |
PGMA (C) | 2.1 | 2.61 | 1.16 | 1.05 |
PGMA (D) | 10.02 | 0.76 | 2.23 | 1.81 |
2.2. Methods
2.2.1. Imaging of Magnetic Particles
2.2.2. Quantitative PCR
3. Results and Discussion
3.1. Determination of Microsphere Surface Morphology
3.2. Microsphere Interference in Quantitative PCR
Microsphere | Microsphere concentration (mg mL−1) | Cq values | ||||
---|---|---|---|---|---|---|
microsphere volume (µL 25 µL−1 PCR mixture) | ||||||
0.0 | 0.5 | 1.0 | 2.5 | 5.0 | ||
P(HEMA-co-GMA) (A1) | 20 | 12.8 ± 0.6 | 14.1 ± 0.3 | 15.3 ± 0.4 | 17.2 ± 0.6 | 27.5 ± 5.9 |
2 | 11.8 ± 0.0 | 11.9 ± 0.0 | 12.4 ± 0.0 | 12.7 ± 0.0 | 13.1 ± 0.0 | |
P(HEMA-co-GMA) (A2) * | 20 | 12.9 ± 0.2 | 15.1 ± 0.4 | 15.7 ± 2.1 | 12.1 ± 1.2 | 10.4 ± 0.7 |
2 | 12.6 ± 0.1 | 13.2 ± 0.2 | 13.5 ± 0.3 | 14.8 ± 0.1 | 15.1 ± 0.3 | |
PGMA (B1) | 20 | 13.0 ± 0.1 | 13.6 ± 0.2 | 13.7 ± 0.2 | 14.3 ± 0.3 | 14.7 ± 0.2 |
2 | 12.6 ± 0.0 | 12.9 ± 0.3 | 12.7 ± 0.0 | 13.2 ± 0.0 | 13.5 ± 0.0 | |
PGMA (B2) * | 20 | 12.9 ± 0.3 | 14.9 ± 0.9 | 15.8 ± 1.1 | 22.8 ± 4.8 | 16.0 ± 1.2 |
2 | 12.8 ± 0.2 | 13.4 ± 0.0 | 14.4 ± 0.0 | 15.9 ± 0.4 | 15.8 ± 2.4 | |
PGMA (C) * | 20 | 12.6 ± 0.6 | 13.6 ± 1.1 | n | n | n |
2 | 12.2 ± 0.2 | 12.3 ± 0.4 | 12.7 ± 0.7 | 13.1 ± 1.0 | 15.6 ± 3.4 | |
PGMA (D) * | 20 | 12.8 ± 0.4 | 14.9 ± 0.7 | 15.4 ± 0.6 | 23.1 ± 5.5 | n |
2 | 12.3 ± 0.1 | 12.7 ± 0.3 | 13.2 ± 0.0 | 13.6 ± 0.0 | 14.0 ± 0.1 |
4. Conclusion
Acknowledgements
Author Contributions
Conflicts of Interest
References
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Trachtová, Š.; Zapletalová, H.; Španová, A.; Horák, D.; Kolářová, H.; Rittich, B. The Evaluation of Magnetic Polymethacrylate-based Microspheres Used for Solid Phase DNA Micro-Extraction. Chromatography 2015, 2, 156-166. https://doi.org/10.3390/chromatography2020156
Trachtová Š, Zapletalová H, Španová A, Horák D, Kolářová H, Rittich B. The Evaluation of Magnetic Polymethacrylate-based Microspheres Used for Solid Phase DNA Micro-Extraction. Chromatography. 2015; 2(2):156-166. https://doi.org/10.3390/chromatography2020156
Chicago/Turabian StyleTrachtová, Štěpánka, Hana Zapletalová, Alena Španová, Daniel Horák, Hana Kolářová, and Bohuslav Rittich. 2015. "The Evaluation of Magnetic Polymethacrylate-based Microspheres Used for Solid Phase DNA Micro-Extraction" Chromatography 2, no. 2: 156-166. https://doi.org/10.3390/chromatography2020156