Detection of Periodontal Pathogens Based on an Integrated Continuous Flow PCR and Capillary Electrophoresis Microfluidic Chip

Yang, Bo; Huang, Jiaxin; Tao, Chunxian; Li, Zhenqing; Zhang, Dawei; Yamaguchi, Yoshinori

doi:10.3390/separations10040271

Open AccessCommunication

Detection of Periodontal Pathogens Based on an Integrated Continuous Flow PCR and Capillary Electrophoresis Microfluidic Chip

by

Bo Yang

^1,2,3,†,

Jiaxin Huang

^1,2,3,†,

Chunxian Tao

^1,2,3,

Zhenqing Li

^1,2,3,*

,

Dawei Zhang

^1,2,3 and

Yoshinori Yamaguchi

^4,*

¹

Engineering Research Center of Optical Instrument and System, Shanghai Environmental Biosafety Instruments and Equipment Engineering Technology Research Center, School of Optoelectronic Information and Computer Engineering, University of Shanghai for Science and Technology, Shanghai 200093, China

²

Key Lab of Optical Instruments and Equipment for Medical Engineering, School of Optoelectronic Information and Computer Engineering, University of Shanghai for Science and Technology, Shanghai 200093, China

³

Shanghai Key Lab of Modern Optical System, School of Optoelectronic Information and Computer Engineering, University of Shanghai for Science and Technology, Shanghai 200093, China

⁴

Graduate School of Engineering, Osaka University, Osaka 565-0871, Japan

^*

Authors to whom correspondence should be addressed.

^†

These authors contributed equally to this work.

Separations 2023, 10(4), 271; https://doi.org/10.3390/separations10040271

Submission received: 8 March 2023 / Revised: 6 April 2023 / Accepted: 17 April 2023 / Published: 21 April 2023

Download

Browse Figures

Versions Notes

Abstract

Continuous-flow PCR (CF-PCR) can realize rapid DNA amplification because of the high temperature variation rate. However, off-line detection methods for PCR may induce cross contamination. To overcome this problem, we herein fabricated an integrated CF-PCR and electrophoresis microfluidic chip. The optimal voltage applied in the electrophoresis part of the microfluidic chip was achieved by simulation in COMSOL. Coating the inside wall of the microchannel can inhibit electroosmotic flow and improve the resolution for DNA fragments. The temperature distribution of the serpentine part can meet the PCR and has no obvious suppressive effect on sample separation. Finally, we have performed the amplification of target genes for Porphyromonas gingivalis, Tannerella forsythia, and Treponema denticola and detected the corresponding PCR products in the microfluidic chip within 11 min. Such work provides a new method for the rapid detection of bacteria.

Keywords: capillary electrophoresis; periodontal pathogens; continuous flow PCR; microfluidic chip; fluorescence detection

Share and Cite

MDPI and ACS Style

Yang, B.; Huang, J.; Tao, C.; Li, Z.; Zhang, D.; Yamaguchi, Y. Detection of Periodontal Pathogens Based on an Integrated Continuous Flow PCR and Capillary Electrophoresis Microfluidic Chip. Separations 2023, 10, 271. https://doi.org/10.3390/separations10040271

AMA Style

Yang B, Huang J, Tao C, Li Z, Zhang D, Yamaguchi Y. Detection of Periodontal Pathogens Based on an Integrated Continuous Flow PCR and Capillary Electrophoresis Microfluidic Chip. Separations. 2023; 10(4):271. https://doi.org/10.3390/separations10040271

Chicago/Turabian Style

Yang, Bo, Jiaxin Huang, Chunxian Tao, Zhenqing Li, Dawei Zhang, and Yoshinori Yamaguchi. 2023. "Detection of Periodontal Pathogens Based on an Integrated Continuous Flow PCR and Capillary Electrophoresis Microfluidic Chip" Separations 10, no. 4: 271. https://doi.org/10.3390/separations10040271

APA Style

Yang, B., Huang, J., Tao, C., Li, Z., Zhang, D., & Yamaguchi, Y. (2023). Detection of Periodontal Pathogens Based on an Integrated Continuous Flow PCR and Capillary Electrophoresis Microfluidic Chip. Separations, 10(4), 271. https://doi.org/10.3390/separations10040271

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

Article Menu

Detection of Periodontal Pathogens Based on an Integrated Continuous Flow PCR and Capillary Electrophoresis Microfluidic Chip

Abstract

Share and Cite

Article Metrics

Article Access Statistics

Further Information

Guidelines

MDPI Initiatives

Follow MDPI