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Article

An Ultra-Fast and Green LC-MS Method for Quantitative Analysis of Aesculin and Aesculetin in Cortex Fraxini

1
School of Pharmaceutical Sciences, Sun Yat-sen University, No.66, Gongchang Road, Guangming District, Shenzhen 518107, China
2
Key Laboratory of State Administration of Traditional Chinese Medicine, Dongguan HEC Cordyceps R&D Co., Ltd., No.368, Zhen’an Middle Road, Chang’an Town, Dongguan 523850, China
*
Authors to whom correspondence should be addressed.
These authors contributed equally to this work.
Separations 2023, 10(9), 515; https://doi.org/10.3390/separations10090515
Submission received: 2 August 2023 / Revised: 5 September 2023 / Accepted: 13 September 2023 / Published: 19 September 2023
(This article belongs to the Special Issue Recent Advances in Green Extraction and Separation Processes)

Abstract

This study aims to develop a fast and eco-friendly liquid chromatography–mass spectrometry (LC-MS) method for the determination of aesculin and aesculetin in Cortex Fraxini. Ultrapure water was used as the solvent during the microwave-assisted extraction process to prepare the Cortex Fraxini sample. This extraction method reduces the cost of the harmful solvent (only ultrapure water was used) and microwave extraction time (1 min). The LC separation was conducted using an Agilent InfinityLab Poroshell 120 EC-C18 column (2.1 mm × 30 mm, 2.7 µm) with a mobile phase consisting of 0.1% formic acid and acetonitrile (90:10, v/v) at a flow rate of 0.6 mL/min. Isocratic elution was employed, and the analytes were detected by MS. Through careful optimization and selection of LC-MS conditions, the analysis time was reduced to 1 min, demonstrating the method’s efficiency. The developed method was validated and exhibited excellent specificity, linearity, limit, precision, accuracy, and stability in quantifying aesculin and aesculetin in the Cortex Fraxini samples. The analysis result revealed the presence of aesculin (ranging from 3.55 to 18.8 mg/g) and aesculetin (ranging from 1.01 to 16.2 mg/g) in all ten batches of Cortex Fraxini samples. Compared to the reported LC methods, this approach substantially reduces the total analysis time and requires a minuscule volume of organic solvents. An “Analytical Eco-Scale” assessment was used to evaluate the different assay methods of Cortex Fraxini. The current LC-MS method scored an impressive 90; it was better than the other four reports’ LC methods. Thus, the developed LC-MS method is rapid and green, which is helpful for the quality evaluation of Cortex Fraxini.
Keywords: LC-MS; Cortex Fraxini; aesculin; aesculetin; green; fast LC-MS; Cortex Fraxini; aesculin; aesculetin; green; fast

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MDPI and ACS Style

Wang, X.; Wang, W.; Lei, Q.; Qian, Z.; Deng, W. An Ultra-Fast and Green LC-MS Method for Quantitative Analysis of Aesculin and Aesculetin in Cortex Fraxini. Separations 2023, 10, 515. https://doi.org/10.3390/separations10090515

AMA Style

Wang X, Wang W, Lei Q, Qian Z, Deng W. An Ultra-Fast and Green LC-MS Method for Quantitative Analysis of Aesculin and Aesculetin in Cortex Fraxini. Separations. 2023; 10(9):515. https://doi.org/10.3390/separations10090515

Chicago/Turabian Style

Wang, Xiaodong, Wenhao Wang, Qinggui Lei, Zhengming Qian, and Wenbin Deng. 2023. "An Ultra-Fast and Green LC-MS Method for Quantitative Analysis of Aesculin and Aesculetin in Cortex Fraxini" Separations 10, no. 9: 515. https://doi.org/10.3390/separations10090515

APA Style

Wang, X., Wang, W., Lei, Q., Qian, Z., & Deng, W. (2023). An Ultra-Fast and Green LC-MS Method for Quantitative Analysis of Aesculin and Aesculetin in Cortex Fraxini. Separations, 10(9), 515. https://doi.org/10.3390/separations10090515

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