Alternative Method for HDL and Exosome Isolation with Small Serum Volumes and Their Characterizations
Abstract
:1. Introduction
2. Materials and Methods
2.1. Materials
2.2. Samples
2.3. HDL Isolation
2.4. Exosome Isolation
2.5. Sodium Dodecyl Sulphate-Polyacrylamide Gel Electrophoresis (SDS-PAGE) and Western Blot
2.6. Exosome Size Analysis
2.7. FTIR Spectral Acquisition
2.8. Principal Component Analysis (PCA)
2.9. Quick Compare Analysis
2.10. Statistical Analysis
3. Results
3.1. HDL Level and Percent Recovery
3.2. Protein Study of Isolated HDL
3.3. HDL Similarity Study by Principal Component Analysis (PCA) and Quick Compare Analysis
3.4. Exosome Size Analysis and Protein Concentration
3.5. SDS-PAGE and Western Blot of Exosomes
3.6. Exosome Similarity Based on PCA and Quick Compare Analysis
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Sample | % Similarity |
---|---|
Exo50 vs. Exo100 | 99.57 ± 0.15% |
Exo50 vs. Exo250 | 99.27 ± 0.18% |
Exo100 vs. Exo250 | 99.43 ± 0.32% |
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Praja, R.K.; Phoksawat, W.; Tippayawat, P.; Jumnainsong, A.; Leelayuwat, C. Alternative Method for HDL and Exosome Isolation with Small Serum Volumes and Their Characterizations. Separations 2021, 8, 204. https://doi.org/10.3390/separations8110204
Praja RK, Phoksawat W, Tippayawat P, Jumnainsong A, Leelayuwat C. Alternative Method for HDL and Exosome Isolation with Small Serum Volumes and Their Characterizations. Separations. 2021; 8(11):204. https://doi.org/10.3390/separations8110204
Chicago/Turabian StylePraja, Rian Ka, Wisitsak Phoksawat, Patcharaporn Tippayawat, Amonrat Jumnainsong, and Chanvit Leelayuwat. 2021. "Alternative Method for HDL and Exosome Isolation with Small Serum Volumes and Their Characterizations" Separations 8, no. 11: 204. https://doi.org/10.3390/separations8110204