Hydrogel Microparticles as Sensors for Specific Adhesion: Case Studies on Antibody Detection and Soil Release Polymers
Abstract
:1. Introduction
2. Results and Discussion
2.1. Synthesis and Functionalization of SCPs with Adhesion Molecules
2.2. Detection of Antibodies with a Combined SCP Pull-Down and Adhesion Assay
2.3. Characterization of Soil Release Polymers by SCP Adhesion Assays
3. Conclusions
4. Experimental Section
4.1. Materials
4.2. Soft Colloidal Probe Preparation
4.3. SCP Characterization
4.4. Surface Preparation
4.5. Determination of Functionalization Degree via TBO Titration
4.6. Reflection Interference Contrast (RICM) Measurements
Supplementary Materials
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Polymer | Adhesion Energies [µJ m−2] | ||
---|---|---|---|
Antiredeposition | Antiadhesive Coating | Direct Binding | |
none/reference | 1700 a | ||
Poly(propylene terephthalate)-co-Poly(ethylene glycol) (nonionic) PPT-co-PEG | 41 | 1038 | 38 b |
Copolymer A: cationic/neutral hydrophilic ratio 22:78 | 163 | 480 | 91 |
Copolymer B: cationic/neutral hydrophilic ratio 70:30 | 306 | 1020 | 126 |
Poly(acrylamide) | 1142 | 1429 | 243 |
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Strzelczyk, A.K.; Wang, H.; Lindhorst, A.; Waschke, J.; Pompe, T.; Kropf, C.; Luneau, B.; Schmidt, S. Hydrogel Microparticles as Sensors for Specific Adhesion: Case Studies on Antibody Detection and Soil Release Polymers. Gels 2017, 3, 31. https://doi.org/10.3390/gels3030031
Strzelczyk AK, Wang H, Lindhorst A, Waschke J, Pompe T, Kropf C, Luneau B, Schmidt S. Hydrogel Microparticles as Sensors for Specific Adhesion: Case Studies on Antibody Detection and Soil Release Polymers. Gels. 2017; 3(3):31. https://doi.org/10.3390/gels3030031
Chicago/Turabian StyleStrzelczyk, Alexander Klaus, Hanqing Wang, Andreas Lindhorst, Johannes Waschke, Tilo Pompe, Christian Kropf, Benoit Luneau, and Stephan Schmidt. 2017. "Hydrogel Microparticles as Sensors for Specific Adhesion: Case Studies on Antibody Detection and Soil Release Polymers" Gels 3, no. 3: 31. https://doi.org/10.3390/gels3030031