A Collagen-Mimetic Organic-Inorganic Hydrogel for Cartilage Engineering
Abstract
:1. Introduction
2. Results and Discussion
2.1. Synthesis and Characterization of the Hybrid Peptides
2.2. Hydrogel Formation, Gelation Time and the Addition of the Mono-Silylated Collagen-Like Peptide
2.3. Structural and Mechanical Characterization of the Hydrogels
2.4. Biological Evaluation of the Hydrogels for Cell Encapsulation
3. Conclusions
4. Materials and Method
4.1. Materials
4.2. Peptide Synthesis
4.3. Peptide Silylation on the Lysine Side Chains
4.4. Circular Dichroism Analyses
4.5. Preparation of the Hybrid Hydrogels
4.6. Cryo-SEM Images
4.7. Indentation Measurements
4.8. Swelling Studies
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
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Hybrid Peptide | Concentration | Gelation Time | Hydrogel Aspect |
---|---|---|---|
6M-2Si | 7 wt% | - a | Above solubility |
6M-2Si | 6 wt% | 6 h 30 min–7 h 30 min | White opaque |
6M-2Si | 5 wt% | > 8 h | Very weak, white opaque |
6M-2Si | 4 wt% | - b | - |
6M-2Si + 6M-1Si | 6 wt% (95/5) | 6 h 30 min–7 h | White opaque |
6M-2Si + 6M-1Si | 6 wt% (90/10) | 6 h 30 min–7 h | White opaque |
6M-2Si + 6M-1Si | 6 wt% (85/15) | - b | - |
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Valot, L.; Maumus, M.; Brunel, L.; Martinez, J.; Amblard, M.; Noël, D.; Mehdi, A.; Subra, G. A Collagen-Mimetic Organic-Inorganic Hydrogel for Cartilage Engineering. Gels 2021, 7, 73. https://doi.org/10.3390/gels7020073
Valot L, Maumus M, Brunel L, Martinez J, Amblard M, Noël D, Mehdi A, Subra G. A Collagen-Mimetic Organic-Inorganic Hydrogel for Cartilage Engineering. Gels. 2021; 7(2):73. https://doi.org/10.3390/gels7020073
Chicago/Turabian StyleValot, Laurine, Marie Maumus, Luc Brunel, Jean Martinez, Muriel Amblard, Danièle Noël, Ahmad Mehdi, and Gilles Subra. 2021. "A Collagen-Mimetic Organic-Inorganic Hydrogel for Cartilage Engineering" Gels 7, no. 2: 73. https://doi.org/10.3390/gels7020073