Bovine Serum Albumin Molecularly Imprinted Electrochemical Sensors Modified by Carboxylated Multi-Walled Carbon Nanotubes/CaAlg Hydrogels
Abstract
:1. Introduction
2. Results and Discussion
2.1. Characterizations of BSA Molecularly Imprinted CMWCNT/CaAlg Hydrogel Films
2.2. Mechanical Properties of BSA Molecularly Imprinted CMWCNT/CaAlg Hydrogel Films
2.3. Adsorption Properties of BSA Molecularly Imprinted CMWCNT/CaAlg Hydrogel Films
2.4. Effect of Alginate on BSA Conformational
2.5. Electrochemical Characterization of Different Electrode Surfaces
2.6. Effect of Eluent Times, Adsorption Times, and Eluent pH on DPV Response Currents of the MIP-Modified Electrodes
2.7. Selective Detection and Imprinting Efficiency of the MIP- and NIP-Modified Electrodes
2.8. Reusability, Stability, and Reproducibility of MIP-Modified Electrodes for BSA Detection
2.9. Linear Range, Detection Limit of the Sensor, and Comparison with Other Materials
3. Conclusions
4. Materials and Methods
4.1. Materials
4.2. Preparation of BSA Molecularly Imprinted CMWCNT/CaAlg Hydrogel Films
4.3. Preparation of BSA Molecularly Imprinted CMWCNT/CaAlg Hydrogel-Modified Electrochemical Sensors
4.4. Characterizations
4.5. Electrochemical Testing
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
Abbreviations
NaAlg | sodium alginate |
BSA | bovine serum albumin |
CMWCNT | carboxylated multi-wall carbon nanotube |
CaAlg | calcium alginate |
MIP | molecularly imprinted polymer |
NIP | non-imprinted polymer |
EIS | electrochemical impedance spectroscopy |
DPV | differential pulse voltammetry |
CD | circular dichroism |
Tris | three (hydroxymethyl) aminomethane |
BHb | bovine hemoglobin |
OVA | ovalbumin |
Lys | lysozyme |
IF | imprinting efficiency |
ΔI | current change value |
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Materials | Linear Range (mg mL−1) | Detection Limit (mg mL−1) | References |
---|---|---|---|
MIP microspheres | 1.0 × 10−5–5.0 × 10−3 | 1.5 × 10−6 | [32] |
MIP/Cd Te quantum dots | 3.3 × 10−2–0.66 | 1.0 × 10−3 | [33] |
Carbon dot | 2.0 × 10−2–0.1 | 8.5 × 10−4 | [34] |
CaAlg/CMWCNT hydrogel MIP | 1.0 × 10−6–1.15 × 10−3 | 5.6 × 10−6 | This method |
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Cheng, L.; Guo, Z.; Lin, Y.; Wei, X.; Zhao, K.; Yang, Z. Bovine Serum Albumin Molecularly Imprinted Electrochemical Sensors Modified by Carboxylated Multi-Walled Carbon Nanotubes/CaAlg Hydrogels. Gels 2023, 9, 673. https://doi.org/10.3390/gels9080673
Cheng L, Guo Z, Lin Y, Wei X, Zhao K, Yang Z. Bovine Serum Albumin Molecularly Imprinted Electrochemical Sensors Modified by Carboxylated Multi-Walled Carbon Nanotubes/CaAlg Hydrogels. Gels. 2023; 9(8):673. https://doi.org/10.3390/gels9080673
Chicago/Turabian StyleCheng, Letian, Zhilong Guo, Yuansheng Lin, Xiujuan Wei, Kongyin Zhao, and Zhengchun Yang. 2023. "Bovine Serum Albumin Molecularly Imprinted Electrochemical Sensors Modified by Carboxylated Multi-Walled Carbon Nanotubes/CaAlg Hydrogels" Gels 9, no. 8: 673. https://doi.org/10.3390/gels9080673