Effects of Ripening Phase and Cultivar under Sustainable Management on Fruit Quality and Antioxidants of Sweet Cherry
by
, , , ,
Iulia Mineață
1,
Otilia Cristina Murariu
2,*,
Sorina Sîrbu
1,
Alessio Vincenzo Tallarita
3,
Gianluca Caruso
3 and
Carmen Doina Jităreanu
4,* 1
Research Station for Fruit Growing, 3 Ion Vodă cel Viteaz Street, Miroslava, 707305 Iasi, Romania
2
Department of Food Technology, “Ion Ionescu de la Brad” Iasi University of Life Sciences, M. Sadoveanu Alley, 700490 Iasi, Romania
3
Department of Agricultural Sciences, University of Naples Federico II, Via Università 100, 80055 Naples, Italy
4
Department of Plant Science, “Ion Ionescu de la Brad” Iasi University of Life Sciences, M. Sadoveanu Alley, 700490 Iasi, Romania
*
Authors to whom correspondence should be addressed.
Horticulturae 2024, 10(7), 720; https://doi.org/10.3390/horticulturae10070720
Submission received: 11 June 2024
/
Revised: 30 June 2024
/
Accepted: 3 July 2024
/
Published: 8 July 2024
(This article belongs to the Special Issue Crop Authenticity in Organic Horticultural Production: Recent Studies and Applications)
Abstract
:Sweet cherry grown under sustainable management produces highly valuable fruits, whose quality shows important biochemical and morphological changes during ripening. Research was carried out in Iasi (Romania), with the aim to assess the quality characteristics of the sweet cherry fruits of three cultivars (Van, Andreiaș, Margonia), grown in an inner or outer position inside the tree crown, at the pre-ripening or full ripeness phase. In 2022, the colour component a* showed higher values in cv. Van and Andreias red fruits and in an inner position, whereas the components L* and b* at the full ripeness phase were highest in cv. Margonia. The dry matter and total soluble solids contents increased from the pre-ripening to the full ripeness phase and were highest in cv. Van sweet cherry fruits; the DM of fruit from the outer part of crown was higher than that of fruit from the inner part at the pre-ripening phase. The content of phenolics was the highest in cv. Margonia fruits at the pre-ripening stage and in cv. Van at the full ripeness phase and higher in the inner tree crown zones. The cultivar Margonia generally showed the highest vitamin C content in both years and development phases. The yellow fruit cv. Margonia mostly showed the highest values of chlorophyll a and b. The fruit’s content of carotene, lycopene, and anthocyanins was generally the highest in the red fruits of cv. Andreias. The examined sweet cherry cultivars showed a high variability in fruit nutritional quality and proved to be a rich source of bioactive compounds with antioxidant potential.
1. Introduction
Sweet cherry (Prunus avium L.) is a fruit tree species belonging to the family Rosaceae, particularly appreciated for its fruits which are the first fresh fruits of the year [1] and show premium quality characteristics in terms of colour, sweetness, sourness, firmness, and nutrients that are beneficial to health [2].
The consumer preference for sweet cherries is mainly influenced by taste and colour, but in recent years also by their nutritional value and the content of bioactive compounds such as anthocyanins, polyphenols, carotenoids, vitamins C and E, and antioxidants [3,4]. Moreover, an increasing percentage of consumers have been paying attention to aspects related to the whole production chain’s sustainability in terms of the balance between resource input and yield output, impact on the environment, nutritional value of the products, and respect of workers’ rights.
There are important factors, i.e., the cultivar, climate conditions, ripening stage, and crop management, that influence the content of bioactive compounds, starting from the growth stage of the fruit until the moment of harvesting [3].
Physiologically, sweet cherry is a non-climacteric fruit and goes through three stages of development before ripening: stage I, which begins after flowering and is followed by a rapid growth and division of the mesocarp cells; stage II, with the volume of the fruit augmenting slowly and the endocarp becoming lignified; and stage III, when the fruit’s volume increases quickly again, and ripening occurs with biochemical changes [5,6].
In the last phenological stage of development, i.e., the full ripening, sweet cherry fruits undergo important biochemical and morphological changes, including the enhancement of colour and sugar content [7], which are the main indicators of maturity for commercial harvesting. However, fruit maturity varies within and between single trees and depends on the cultivar, meteorological conditions [8], agronomic practices [9], canopy management, fruit load [10], and fertilization [11]. The mentioned factors need to be examined from the perspective of crop system sustainability, mainly from the technical, nutritional, and environmental points of view.
Physicochemical studies are crucial to appropriately manage pre-harvest and harvest technology for sweet cherry production worldwide [12].
According to previous works related to some Prunus species, variations in quality parameters and fruit size reportedly depend on the position of the fruit within the crown of the tree [13,14]. Particularly, the location should be taken into account of both the fruit inside the tree, i.e., the sunny or shady side, and the tree within the orchard [15].
Previous studies [9,16,17,18] highlighted that the ripening stage influences the quality of sweet cherries by visibly changing the fruit colour and skin intensification, measurable by the parameters L* (luminosity), a* (red–green), and b* (yellow–blue), which turn from green to red or yellow depending on the cultivar, as chlorophyll pigments are degraded and anthocyanins accumulate.
Dry matter and total soluble solids contents increase during the development of fruits [18,19], which become much sweeter during ripening due to the sugar concentration increase, while acids (malic acid) remain relatively constant or decrease [1].
The purpose of this study was to evaluate the effects of the cultivar and position of the fruits inside the tree crown, at the pre-ripening or full ripeness phases, on the quality parameters of organically grown sweet cherry fruits.
2. Materials and Methods
2.1. Experimental Protocol and Growing Conditions
Research was carried out in 2022 and 2023 at the experimental field of the Research Station for Fruit Growing (RSFG) in Iași, North-East Romania (47°20′ N; 27°60′ E and 165 m altitude), in a sweet cherry orchard planted in a meso-relief with a gently sloping plateau and predominantly cambic chernozem soil with good natural drainage [20], pH 7.3, 4.0% humus content, and 0.19% N.
The meteorological conditions at the experimental field during the research years (2022–2023) were monitored by an Agroexpert system and are presented in Table 1.
The experimental protocol was based on the factorial combination between three cultivars (Van, Andreiaș, Margonia) and two fruit positions inside the tree crown (inner, outer). A split plot design was used for the treatment distribution in the field, with three replicates, with a density of 500 plants ha−1.
Sweet cherry samples were harvested from the sampled trees at two different fruit development phases: pre-ripening, with fruit at about 90% of their final size and beginning colouring; and full ripeness.
The three cultivars of sweet cherry (Van, Andreiaș, and Margonia) were provided by the Experimental Orchard Fields of RSFG, Iași. The trees of the mentioned cultivars were reared on high-vigour rootstocks of Prunus mahaleb L., spaced 5 m along the rows, which were 4 m apart, and trained as a free palmette. The soil was ploughed using a rotary tiller along the rows and covered by grass between the rows.
The cultivar Van originated from Canada and is an early and very productive cultivar, with medium to large fruits (7.5–8.5 g), a globular shaped, bright red colour, stony pink-red flesh, and a sweet acidic taste [21].
Andreiaș and Margonia are two new cultivars of sweet cherry, created and certified at RSFG Iași. Andreiaș has large (8.5–9.5 g), cordiform, red-brown fruit, almost black when fully ripe, and the cultivar Margonia has yellow, cordiform, slightly flattened fruit, elongated at the tip, with an average weight of 8.0–9.0 g [22].
The crops were organically managed [23], using the formulations permitted under the European regulation both for fertilization and plant protection.
2.2. Sampling Procedures
Fruit samples were harvested in all the plots, put in biodegradable bags, and immediately transported under appropriate conditions to the laboratory for analysis (Figure 1).
2.3. Qualitative Analyses
2.3.1. Measurement of Epidermis Colour
The fruit’s epidermis colour was measured using a Minolta CR 400/410C portable colorimeter (Konica Minolta Measuring Instruments, München, Germany), referring to the CIE L* a* b* uniform colour space. The value of L* reflects the colour lightness, a* the proportions of red (positive values) and green (negative values), and b* the proportion of yellow (positive values) and blue (negative values). The parameter L* defines the colour intensity, while a* and b* define the colour chromaticity [24,25].
2.3.2. Total Soluble Solids (TSS)
TSS content was determined by the refractive index of the fruit juice, measured using a Zeiss precision portable refractometer (Jena, Germany), and reported in °Brix.
2.3.3. Dry Matter
The total dry matter content was measured by drying 5 g of fruit samples (without stone) in an oven at 103 ± 2 °C until constant weight [26].
2.3.4. pH and Titratable Acidity
The pH value of the liquid samples was determined using the potentiometric method using a Hanna Instruments (Laval, QC, Canada) pH meter at 20 °C. Due to the similarity of the liquid fruit sample preparation, pH and titratable acidity (TA) were measured simultaneously [27].
To determine the titratable acidity (TA), the samples were homogenized with distilled water and titrated with 0.1 N NaOH until reaching pH 8.1. The results were expressed as percentage of malic acid content and calculated using Formula (1) below:
where N is the normality of NaOH; V is the volume of NaOH (mL) used in the titration; m is the weight of the sweet cherry sample used (g); and 0.0067 is the conversion factor for malic acid [12]. TA has been calculated in terms of the most common acid in the sample, which for different cultivars of sweet cherry is usually malic acid [15].
TA (% malic acid) = (V × N × 100 × 0.0067)/m
2.3.5. Carotene and Lycopene
The identification of carotene and lycopene in sweet cherry fruits (both peel and pulp) was carried out spectrophotometrically with petroleum ether. The ether extract was read by a UV/Vis Specord 210 Plus spectrophotometer (Analytik Jena, Jena, Germany) at 472 nm for lycopene and 452 nm for carotene. The obtained values were integrated into the following formulas [28].
Carotene = A452 × 19.96, in mg 100 g−1
Lycopene = A472 × 14.495, in mg 100 g−1
Lycopene = A472 × 14.495, in mg 100 g−1
2.3.6. Anthocyanin Content
2.3.7. Chlorophyll Pigments
The content of chlorophyll pigments (chlorophyll a and b) in the sweet cherry fruits was determined by the spectrophotometric method described by Jităreanu et al. [32], based on the light absorption capacity of the acetonic extract (1%) and analysed by a computer-aided spectrophotometer at 647 and 663 nm absorption based on Lichtenthaler’s equations [33,34]:
Ca = 12.25 × A663 − 2.79 ×A647
Cb = 21.50 × A647 − 5.10 × A663
Ca + b = 7.15 × A663 − 18.71 × A647
Cb = 21.50 × A647 − 5.10 × A663
Ca + b = 7.15 × A663 − 18.71 × A647
2.3.8. Total Phenolic Content
The determination of the content of total phenolic compounds was carried out according to the Folin–Ciocalteu [35] by reading values on a spectrophotometer at a 750 nm wavelength. The results reveal the antioxidant activity and are expressed as mg of gallic acid equivalents (GAEs) per 100 g of sample [36].
2.3.9. Vitamin C
In order to determine the vitamin C content of the sweet cherry samples, the 2.6-dichloroindophenol titrimetric method of juice analysis was used (AOAC Method 967.21) [37]. Samples were fused with 40 mL of 2% oxalic acid, and after titration (2.6-dichloroindophenol), the results were expressed in mg ascorbic acid 100 g−1 fruit [38].
2.4. Statistical Analysis
The data were statistically processed by two-way analysis of variance, and Duncan’s multiple range test was performed for mean separation at p ≤ 0.05 using the SPSS software version 29. The standard deviation (STDEV) and the Pearson coefficient of variation (COVAR) were also determined [39].
3. Results and Discussion
As no significant interactions arose between the two experimental factors applied, i.e., cultivar and fruit position inside the tree crown, only their simple effects are presented.
The colour of fresh sweet cherry fruits is among the primary factors influencing consumer choice, because it is an important quality attribute, also serving to estimate the maturity and harvesting stage [40].
With regard to the fruit’s chromatic parameters (L*, a*, b*), in 2022, the component a* showed significantly higher values in the red fruits of the cultivars Van and Andreias and in the inner position inside the tree crown, both at the pre-ripening and full ripeness phase (Table 2). In the same year, the components L* and b* were significantly affected by cultivar only at the full ripeness phase, with the highest levels recorded in the yellow fruits of the cultivar Margonia. In 2023 (Table 3), the cultivars Van and Margonia attained higher values of the three colour components at the pre-ripening phase, whereas similar trends to the previous year were recorded at the full ripeness phase. No colour components were significantly influenced by the fruit’s position inside the tree crown.
Compared to the year 2022, in the meteorological conditions recorded in 2023, there was generally a decrease in the values of colour parameters of Andreias fruits at the pre-ripening phase; moreover, the component a* was not affected by the fruit’s position inside the crown.
As for the comparison between the two development phases, the yellow fruits of the cultivar Margonia showed a stability of values, whereas the red fruits of cv. Van and Andreias exhibited a decrease in L* and b* components and an increase in the a* component during the ripening process.
The differences between the two phenological phases or between the inner and outer crown area can be useful in evaluating the fruit ripening process to compare the quality of cultivars and the influence of climatic conditions and to determine the time required to reach full maturity. In this respect, compared to the year 2022, in 2023, the time interval from the pre-ripening stage to full ripening was longer due to the lower average temperature, which caused a slower but more efficient accumulation of anthocyanin pigments in the red fruits, associated with higher values of the a* parameter.
The chromatic values recorded in our investigation are consistent with those detected by Goncalves et al. [41] in sweet cherry fruits of the cultivar Van at the partial and full ripeness phases. The results of the present research are also similar to those obtained by Girad and Kopp [42], who stated that darker sweet cherries (lower L* values) tended to be less red (lower a* value) and less yellow (lower b* value). The maximum values of the colour component L* corresponded to the minimum values of the hue parameters a* and b*. Similar colour variations during fruit ripening also occurred in sour cherries, with the skin colour parameters L*and b* decreasing uniformly [40,43].
Both in 2022 (Table 4) and in 2023 (Table 5), at the two development phases examined, the dry matter (DM) and total soluble solids (TSS) contents of cv. Van sweet cherry fruits were highest (on average, 17.7% and 12.9 °Brix, respectively), except in 2022, when at the full ripeness phase, the DM of Van did not significantly differ from that of Andreias. Moreover, the DM of the fruit in the outer brighter part of crown was significantly higher than in the inner part in both years but only at the pre-ripening phase.
Either the dry matter or the soluble solids content of sweet cherry fruits of the three cultivars examined always increased from the pre-ripening to the full ripeness phase, independently of the year, with the top values being those of cv. Van in 2023 (22.7% and 15.3 °Brix).
Total soluble solids (TSS), predominantly estimating the level of dissolved sugars (sucrose, glucose, and fructose) in fruit juice but also including a small number of other compounds such as organic acids, soluble amino acids, and minerals [15], are an important quality parameter that has a remarkable influence on the fruit taste.
Consistently with our results, in previous research carried out on sweet cherry cultivars, both the DM and TSS contents increased during the fruit ripening, in the same agricultural area and with the same cultivars as in the present experiment [44] or in other locations [17,45,46,47].
Many scientific investigations have shown that the TSS vary between 11 °Brix and 25 °Brix in sweet cherries in several areas of the world. In the Spanish cultivars Sweetheart and Picota, a TSS range of 14.0–23.2 °Brix was reported by Serradilla et al. [48] and Gonzalez-Gomez et al. [49]. In the cultivars Bing and Summit, average TSS values of 13.3 and 19.6 °Brix were recorded, respectively [12]. Moreover, Wen et al. [50] detected TSS values between 17.8 and 20.0 °Brix for the Chinese sweet cherry cultivars Rainier, Hongyan, and Hongdeng.
In previous research, the meteorological trend in the last month of fruit formation exerted the greatest influence on TSS accumulation in sweet cherry fruit, regardless of the ripening phase [51,52], which has an important impact on the flavour evaluation [53].
Important physicochemical parameters, such as pH, titratable acidity (TA), and nutraceuticals (total polyphenols, ascorbic acid, and pigments) influence the consumer’s perception of fruit quality [18,44].
Neither the pH or titratable acidity values in the sweet cherry fruits (on average, 3.87 and 1.04 g 100 g−1 malic acid, respectively) were significantly influenced by the cultivar and ripening process, both in 2022 and 2023 (Table 6 and Table 7). In previous research, sweet cherry juice had a higher pH than the sour one [54,55], and the levels of both the juice’s pH and total acidity were proven to also be genotype-dependant [56].
Acidity is essential for the consumer’s perception of a good sweet cherry flavour [11].
According to other studies carried out on sweet cherries [45,46,47], TA widely ranged between 0.39% and 0.87%, but based on Crisosto et al.’s reports [16], the framing limits of TA in ripe sweet cherries vary from 0.5 to 1.0 mg malic acid 100 g−1 f.w.
In both studied years, the contents of phenolic compounds (Table 6 and Table 7) were significantly the highest in cv. Margonia fruits at the pre-ripening phase (on average, 182.7 mg GAE 100 g−1 f.w.) and cv. Van at the full ripeness phase (on average, 76.5 mg GAE 100 g−1 f.w.). Moreover, on average, phenolics showed an over 50% decrease from the pre-ripening to the full ripeness phase in both years.
The polyphenol content was higher in the fruits located in the inner part of the crown but only at the full ripeness phase.
Sweet cherry fruits are considered a very good source of phenolic compounds that are beneficial in a human diet [51]. In the present experiment, the variation in polyphenols contents in fruits mainly depended on the examined cultivars and development phase.
Similar values of TP as in the present research were detected in the ripe fruits of some sweet cherry cultivars by other authors [51,57,58], but in the premature stage, the fruits had a much higher content.
Sweet cherry fruits are appreciated by consumers for their good taste but also for their many healthy nutritional properties, such as the high vitamin C content [51].
As can be observed in Table 6 and Table 7, in our study, the cultivar Margonia showed the highest vitamin C content in both years and development phases (14.7 mg·100 g−1 f.w., on average), though no significant differences between the cultivars were found at the full ripeness phase in 2022. Only in the latter case, a significant difference was recorded between the positions inside the crown, because the outer sweet cherry fruits had higher vitamin C contents than the inner ones. The fully ripe fruits showed a more than doubled vitamin C content compared to the unripe ones.
Contrary to the reports by Średnicka-Tober [59], indicating that the fruits of sweet cherry were characterized by a significantly higher content of vitamin C, up to 42.9 mg 100 g−1 f.w., in other studies [51,60], depending on the cultivar and the meteorological conditions of the research year, the average content of vitamin C in sweet cherries varied between 5.3 and 20.3 mg 100 g−1 f.w.
The phenological phase of full ripeness in sweet cherry fruits influences the content of secondary compounds, such as pigments, which enhance the nutritional and health value of a fruit and, in addition, their evolution visually appeals consumers in terms of fruit colouration [61].
As for the fruit’s chlorophyll pigments, in both years (Table 8 and Table 9), the yellow fruit cv. Margonia showed the highest values of chlorophyll a and b, either at the pre-ripening (22.0 mg 100 g−1 f.w. chlorophyll a and 36.9 mg 100 g−1 f.w. chlorophyll b, on average) or at the full ripeness phase (3.9 mg 100 g−1 f.w. chlorophyll a and 6.8 mg 100 g−1 f.w. chlorophyll b, on average), but in 2023 (Table 9), it did not significantly differ from Andreias at the full ripeness phase. Moreover, only in the latter context was a significant difference recorded between the two fruit positions inside the tree crown, with the outer fruits attaining higher chlorophyll a contents than the inner ones.
Differently from chlorophyll pigments, in both years (Table 8 and Table 9), the fruit’s contents of carotene and lycopene were highest in the red fruits of cv. Andreias (0.39 mg 100 g−1 f.w. carotene and 0.31 mg 100 g−1 f.w. lycopene, as an average of the year and development phase), but in 2022 at the full ripeness phase, the carotene level did not significantly differ from that of cv. Margonia. The anthocyanin trend was exactly the same as that of lycopene, but its values were much higher at the full ripeness phase in both years (38.2 mg 100 g−1 f.w., on average) compared to the pre-ripening phase (1.5 mg 100 g−1 f.w., on average).
As for the comparison between the two positions inside the tree crown, the content of lycopene was higher in the outer fruits only in 2023 at both the development phases; the anthocyanin level was higher in the outer fruits at the pre-ripening phase only in 2022, and in the inner ones at the full ripeness phase in both years.
The total contents of carotene and lycopene during the ripening period did not univocally change and, according to Abers and Wrolstad’s study [62], they can decrease due to the augmentation of the total anthocyanins, which tend to mask the colour of carotenoids and degrade the chlorophyll due to the development of dark pigmented compounds.
Anthocyanins, belonging to the subclass of water-soluble flavonoids, are one of the main phenolic compounds in sweet cherries and is responsible for the red colouring of the fruit epidermis and pulp [15] and associated with the high antioxidant potential of sweet cherry fruits [63].
During the two years of the present study, a remarkable increase in the content of anthocyanins from the pre-ripening to full ripeness phase was recorded in the cultivars with red fruits (Van and Andreiaș), whereas these compounds showed a low accumulation in the yellow fruits of cv. Margonia at the full ripeness phase.
In previous studies regarding conventionally grown sweet cherry fruits, a variability of anthocyanins arose between cultivars, from 12.1 (cultivar Elton Heart) to 91.6 mg 100 g−1 f.w. (cv. Kordia) [59]. Ferretti et al. [60] showed that cv. Regina fruits contained 41.0 mg 100 g−1 f.w. anthocyanins, and in another study [51], 54.0 mg 100 g−1 f.w. anthocyanins were detected in red sweet cherry fruits on average.
4. Conclusions
In the context of sustainable production management carried out in the present research, significant quality differences arose between the organically grown Prunus avium L. fruits of the examined cultivars, which proved to be a very good source of biologically active compounds with beneficial nutritional value to human health.
When the fruits ripened and the colour changed, increases were recorded in the values of the quality indices that are highly appreciated by consumers, such as the content of soluble sugars, vitamin C, carotenoids, and anthocyanins, although a higher content of polyphenolic compounds was found at the pre-ripening phase.
The biochemical processes involved in the fruit ripening process led to several changes in quality parameters, mainly influenced by the variation in seasonal meteorological conditions and cultivar, but also by the fruit’s position inside the tree crown.
The fruits of the locally created cultivar Andreiaș were the richest in antioxidants, and the latter outcome suggests an opportunity to both valorise and include the mentioned cultivar in breeding strategies under an organic management programme.
Author Contributions
Conceptualization, I.M., O.C.M. and C.D.J.; methodology, I.M. and O.C.M.; software, I.M. and O.C.M.; validation, O.C.M., G.C. and C.D.J.; formal analysis, I.M., A.V.T. and S.S; investigation, I.M., S.S., A.V.T. and O.C.M.; resources, I.M., O.C.M. and C.D.J.; data curation, I.M., O.C.M. and G.C.; writing—original draft preparation, I.M.; writing—review and editing, O.C.M., G.C. and C.D.J.; visualization, O.C.M., G.C. and C.D.J.; supervision, C.D.J. All authors have read and agreed to the published version of the manuscript.
Funding
This research received no external funding.
Data Availability Statement
The original contributions presented in the study are included in the article, further inquiries can be directed to the corresponding authors.
Conflicts of Interest
The authors declare no conflicts of interest.
References
- Habib, M.; Bhat, M.; Dar, B.N.; Wani, A.A. Sweet cherries from farm to table: A review. Crit. Rev. Food Sci. Nutr. 2017, 57, 1638–1649. [Google Scholar] [CrossRef] [PubMed]
- Esti, M.; Cinquanta, L.; Sinesio, F.; Moneta, E.; Di Matteo, M. Physicochemical and sensory fruit characteristics of two sweet cherry cultivars after cool storage. Food Chem. 2002, 76, 399–405. [Google Scholar] [CrossRef]
- Quero-García, J.; Lezzoni, A.; Puławska, J.; Lang, G. Cherries: Botany, Production and Uses; CABI: Wallingford, UK, 2017; Available online: https://www.cabi.org/bookshop/book/9781780648378 (accessed on 22 August 2023).
- Ballistreri, G.; Continella, A.; Gentile, A.; Amenta, M.; Fabroni, S.; Rapisarda, P. Fruit quality and bioactive compounds relevant to human health of sweet cherry (Prunus avium L.) cultivars grown in Italy. Food Chem. 2013, 140, 630–638. [Google Scholar] [CrossRef] [PubMed]
- Andrews, P.K.; Li, S. Partial Purification and Characterization of.beta.-D-Galactosidase from Sweet Cherry, a Nonclimacteric Fruit. J. Agric. Food Chem. 1994, 42, 2177–2182. [Google Scholar] [CrossRef]
- Knoche, M.; Beyer, M.; Peschel, S.; Oparlakov, B.; Bukovac, M.J. Changes in strain and deposition of cuticle in developing sweet cherry fruit. Physiol. Plant. 2004, 120, 667–677. [Google Scholar] [CrossRef]
- Tudela, J.; Luchsinger, L.; Artés-Hdez, F.; Artés, F. ‘Ambrunes’ sweet cherry quality factors change during ripening. Acta Hortic. 2005, 667, 529–534. [Google Scholar] [CrossRef]
- Gonçalves, B.; Landbo, A.-K.; Knudsen, D.; Silva, A.P.; Moutinho-Pereira, J.; Rosa, E.; Meyer, A.S. Effect of Ripeness and Postharvest Storage on the Phenolic Profiles of Cherries (Prunus avium L.). J. Agric. Food Chem. 2004, 52, 523–530. [Google Scholar] [CrossRef] [PubMed]
- Mozetič, B.; Simčič, M.; Trebše, P. Anthocyanins and hydroxycinnamic acids of Lambert Compact cherries (Prunus avium L.) after cold storage and 1-methylcyclopropene treatment. Food Chem. 2006, 97, 302–309. [Google Scholar] [CrossRef]
- Whiting, M.D.; Lang, G.; Ophardt, D. Rootstock and Training System Affect Sweet Cherry Growth, Yield, and Fruit Quality. HortScience 2005, 40, 582–586. [Google Scholar] [CrossRef]
- Crisosto, C.H.; Johnson, R.S.; Day, K.; DeJong, T. Preharvest Factors Affecting Postharvest Stone Fruit Quality. HortScience 1995, 30, 751A. [Google Scholar] [CrossRef]
- Hayaloglu, A.A.; Demir, N. Phenolic compounds, volatiles, and sensory characteristics of twelve sweet cherry (Prunus avium L.) cultivars grown in Turkey. J. Food Sci. 2016, 81, 7–18. [Google Scholar] [CrossRef]
- Lopresti, J.; Goodwin, I.; Holford, P.; McGlasson, B.; Golding, J. Study on the relationship between sugar concentration and cell number and size distribution in nectarine. Acta Hortic. 2015, 1084, 667–674. [Google Scholar] [CrossRef]
- Lopresti, J.; Goodwin, I.; Stefanelli, D.; Holford, P.; McGlasson, B.; Golding, J. Understanding the factors affecting within-tree variation in soluble solids concentration in peaches and nectarines. Acta Hortic. 2016, 1130, 249–256. [Google Scholar] [CrossRef]
- Ricardo-Rodrigues, S.; Laranjo, M.; Agulheiro-Santos, A.C. Methods for quality evaluation of sweet cherry. J. Sci. Food Agric. 2022, 103, 463–478. [Google Scholar] [CrossRef] [PubMed]
- Crisosto, C.H.; Crisosto, G.M.; Metheney, P. Consumer acceptance of ‘Brooks’ and ‘Bing’ cherries is mainly dependent on fruit SSC and visual skin color. Postharvest Biol. Technol. 2003, 28, 159–167. [Google Scholar] [CrossRef]
- Muskovics, G.; Felföldi, J.; Kovács, E.; Perlaki, R.; Kállay, T. Changes in physical properties during fruit ripening of Hungarian sweet cherry (Prunus avium L.) cultivars. Postharvest Biol. Technol. 2006, 40, 56–63. [Google Scholar] [CrossRef]
- Magri, A.; Malorni, L.; Cozzolino, R.; Adiletta, G.; Siano, F.; Picariello, G.; Cice, D.; Capriolo, G.; Nunziata, A.; Di Matteo, M.; et al. Agronomic, Physicochemical, Aromatic and Sensory Characterization of Four Sweet Cherry Accessions of the Campania Region. Plants 2023, 12, 610. [Google Scholar] [CrossRef] [PubMed]
- Kovács, E.; Muskovics, G.; Perlaki, R. Relationship of colour and other quality parameters of sweet cherry during development and ripening. Acta Aliment. 2009, 38, 415–426. [Google Scholar] [CrossRef]
- Iurea, E.; Sîrbu, S.; Corneanu, G.; Corneanu, M. Results obtained from sweet cherry breeding in Iaşi, Romania. J. Appl. Life Sci. Environ. 2022, 187, 333–341. [Google Scholar] [CrossRef]
- Budan, S.; Grădinariu, G. Cireșul. In Ion Ionescu de la Brad; Editura: Iași, Romania, 2000; p. 264. [Google Scholar]
- Corneanu, G.; Cârdei, E.; Corneanu, M.; Sîrbu, S.; Iurea, E. Cercetare, Inovare și Progres în Pomicultura Ieșeană; Editura Altfel: Iași, România, 2012; p. 99. [Google Scholar]
- Regulation (EU) 2018/848 of the European Parliament and of the Council of 30 May 2018 on Organic Production and Labelling of Organic Products; Document 32018R0848; European Union: Brussels, Belgium, 2018.
- Ibraheem, N.A.; Hasan, M.M.; Khan, R.Z.; Mishra, P.K. Understanding Color Models: A Review. ARPN J. Sci. Technol. 2012, 2, 265–275. [Google Scholar]
- Rutkowski, K.; Łysiak, G.P. Weather Conditions, Orchard Age and Nitrogen Fertilization Influences Yield and Quality of ‘Łutówka’ Sour Cherry Fruit. Agriculture 2022, 12, 2008. [Google Scholar] [CrossRef]
- Escribano, S.; Biasi, W.V.; Lerud, R.; Slaughter, D.C.; Mitcham, E.J. Non-destructive prediction of soluble solids and dry matter content using NIR spectroscopy and its relationship with sensory quality in sweet cherries. Postharvest Biol. Technol. 2017, 128, 112–120. [Google Scholar] [CrossRef]
- Murariu, O.C.; Brezeanu, C.; Jităreanu, C.D.; Robu, T.; Irimia, L.M.; Trofin, A.E.; Popa, L.-D.; Stoleru, V.; Murariu, F.; Brezeanu, P.M. Functional Quality of Improved Tomato Genotypes Grown in Open Field and in Plastic Tunnel under Organic Farming. Agriculture 2021, 11, 609. [Google Scholar] [CrossRef]
- Brezeanu, C.; Brezeanu, P.M.; Stoleru, V.; Irimia, L.M.; Lipsa, F.D.; Teliban, G.-C.; Ciobanu, M.M.; Murariu, F.; Puiu, I.; Branca, F.; et al. Nutritional Value of New Sweet Pepper Genotypes Grown in Organic System. Agriculture 2022, 12, 1863. [Google Scholar] [CrossRef]
- Lee, J.; Durst, R.W.; Wrolstad, E.R.; Eisele, T.; Giusti, M.M.; Hofsommer, H.; Koswig, S.; Krueger, A.D.; Kupina, S.; Martin, S.K.; et al. Determination of Total Monomeric Anthocyanin Pigment Content of Fruit Juices, Beverages, Natural Colorants, and Wines by the pH Differential Method: Collaborative Study. J. AOAC Int. 2005, 88, 1269–1278. [Google Scholar] [CrossRef] [PubMed]
- Teng, Z.; Jiang, X.; He, F.; Bai, W. Qualitative and Quantitative Methods to Evaluate Anthocyanins. eFood 2020, 1, 339–346. [Google Scholar] [CrossRef]
- Zdremţan, M. Conservarea Legumelor şi Fructelor—Îndrumător de Lucrări Practice; Editura Universităţii: Bucharest, Romania; Aurel Vlaicu University: Arad, Romania, 2008; p. 153. ISBN 978-973-752-290-0. [Google Scholar]
- Jitareanu, C.D.; Toma, L.D.; Slabu, C.; Marta, A.E. Effect of weather conditions on photosynthetic and flavonoid pigment contents in leaves of grapevine cultivars during growing season. J. Food Agric. Environ. 2011, 9, 793–798. [Google Scholar]
- Lichtenthaler, H.K. Chlorophylls and carotenoids: Pigments of photosynthetic biomembranes. Methods Enzymol. 1987, 148, 350–382. [Google Scholar] [CrossRef]
- Wellburn, A.R. The Spectral Determination of Chlorophylls a and b, as well as Total Carotenoids, Using Various Solvents with Spectrophotometers of Different Resolution. J. Plant Physiol. 1994, 144, 307–313. [Google Scholar] [CrossRef]
- Dziadek, K.; Kopeć, A.; Piątkowska, E.; Leszczyńska, T.; Pisulewska, E.; Witkowicz, R.; Bystrowska, B.; Francik, R. Identification of polyphenolic compounds and determination of antioxidant activity in extracts and infusions of buckwheat leaves. Eur. Food Res. Technol. 2017, 244, 333–343. [Google Scholar] [CrossRef]
- Caruso, G.; Stoleru, V.V.; Munteanu, N.C.; Sellitto, V.M.; Teliban, G.C.; Burducea, M.; Tenu, I.; Morano, G.; Butnariu, M. Quality Performances of Sweet Pepper under Farming Management. Not. Bot. Horti Agrobot. Cluj-Napoca 2019, 47, 458–464. [Google Scholar] [CrossRef]
- AOAC International. Official Methods of Analysis, 20th ed.; AOAC International: Rockville, MD, USA, 2016. [Google Scholar]
- Nielsen, S.S. Vitamin C Determination by Indophenol Method. In Nielsen’s Food Analysis Laboratory Manual; Food Science Text Series; Ismail, B.P., Nielsen, S.S., Eds.; Springer: Cham, Switzerland, 2017; pp. 143–146. [Google Scholar]
- Botu, I.; Botu, M. Tehnica Experimetală în Horticultură și Ecologie (Elemente de Bază); Editura Conphys, Rm.: Vâlcea, România, 2010; p. 139. [Google Scholar]
- Pedišić, S.; Levaj, B.; Dragović-Uzelac, V.; Škevin, D.; Skendrović Babojelić, M. Color parameters and total anthocyanins of sour cherries (Prunus cerasus L.) during ripening. Agric. Conspec. Sci. 2009, 74, 259–262. [Google Scholar]
- Gonçalves, B.; Silva, A.P.; Moutinho-Pereira, J.; Bacelar, E.; Rosa, E.; Meyer, A.S. Effect of ripeness and postharvest storage on the evolution of colour and anthocyanins in cherries (Prunus avium L.). Food Chem. 2007, 103, 976–984. [Google Scholar] [CrossRef]
- Girard, B.; Kopp, T.G. Physicochemical Characteristics of Selected Sweet Cherry Cultivars. J. Agric. Food Chem. 1998, 46, 471–476. [Google Scholar] [CrossRef] [PubMed]
- Molaeafard, S.; Jamei, R.; Marjani, A.P. Co-pigmentation of anthocyanins extracted from sour cherry (Prunus cerasus L.) with some organic acids: Color intensity, thermal stability, and thermodynamic parameters. Food Chem. 2021, 339, 128070. [Google Scholar] [CrossRef]
- Petriccione, M.; Mastrobuoni, F.; Pasquariello, M.S.; Zampella, L.; Nobis, E.; Capriolo, G.; Scortichini, M. Effect of Chitosan Coating on the Postharvest Quality and Antioxidant Enzyme System Response of Strawberry Fruit during Cold Storage. Foods 2015, 4, 501–523. [Google Scholar] [CrossRef]
- Corneanu, M.; Iurea, E.; Sîrbu, S. Biological properties and fruit quality of sweet cherry (Prunus avium L.) cultivars from Rom. Assortment. Agron. Res. 2020, 18, 2353–2364. [Google Scholar] [CrossRef]
- Corneanu, M.; Iurea, E.; Sirbu, S. Comparison of Five New Sweet Cherry Cultivars Bred in Romania, with their Parental Forms. J. Hortic. Res. 2021, 29, 1–8. [Google Scholar] [CrossRef]
- Sîrbu, S.; Beceanu, D.; Corneanu, G.; Petre, L.; Anghel, R.M.; Iurea, E. Fruit quality evaluation of some sweet cherry cultivars in Iasi, Romania. In Lucrări Științifice; Universitatea de Științe Agricole Și Medicină Veterinară Ion Ionescu de la Brad: Iași, Romania, 2009; Volume 52, pp. 419–422. [Google Scholar]
- Serradilla, M.J.; Martín, A.; Ruiz-Moyano, S.; Hernández, A.; López-Corrales, M.; De Guía Córdoba, M. Physicochemical and sensorial characterisation of four sweet cherry cultivars grown in Jerte Valley (Spain). Food Chem. 2012, 133, 1551–1559. [Google Scholar] [CrossRef]
- González-Gómez, D.; Lozano, M.; Fernández-León, M.F.; Bernalte, M.J.; Ayuso, M.C.; Rodríguez, A.B. Sweet cherry phytochemicals: Identification and characterization by HPLC-DAD/ESI-MS in six sweet-cherry cultivars grown in Valle del Jerte (Spain). J. Food Compos. Anal. 2010, 23, 533–539. [Google Scholar] [CrossRef]
- Wen, Y.-Q.; He, F.; Zhu, B.-Q.; Lan, Y.-B.; Pan, Q.-H.; Li, C.-Y.; Reeves, M.J.; Wang, J. Free and glycosidically bound aroma compounds in cherry (Prunus avium L.). Food Chem. 2014, 152, 29–36. [Google Scholar] [CrossRef] [PubMed]
- Hallmann, E.; Rozpara, E. The estimation of bioactive compounds content in organic and conventional sweet cherry (Prunus avium L.). J. Res. Appl. Agric. Eng. 2017, 62, 141–145. Available online: https://bibliotekanauki.pl/articles/336048.pdf (accessed on 28 June 2024).
- Ivanova, I.; Serdiuk, M.; Malkina, V.; Bandura, I.; Kovalenko, I.; Tymoshchuk, T.; Tonkha, O.; Tsyz, O.; Mushtruk, M.; Omelian, A. The study of soluble solids content accumulation dynamics under the influence of weather factors in the fruits of cherries. Potravin. Slovak J. Food Sci. 2021, 15, 350–359. [Google Scholar] [CrossRef]
- Drake, S.R.; Fellman, J.K. Indicators of Maturity and Storage Quality of ‘Rainier’ Sweet Cherry. HortScience 1987, 22, 283–285. [Google Scholar] [CrossRef]
- Siddiq, M.; Iezzoni, A.; Khan, A.; Breen, P.; Sebolt, A.M.; Dolan, K.D.; Ravi, R. Characterization of New Tart Cherry (Prunus cerasus L.): Selections Based on Fruit Quality, Total Anthocyanins, and Antioxidant Capacity. Int. J. Food Prop. 2011, 14, 471–480. [Google Scholar] [CrossRef]
- Chaovanalikit, A.; Wrolstad, R.E. Total Anthocyanins and Total Phenolics of Fresh and Processed Cherries and Their Antioxidant Properties. J. Food Sci. 2004, 69, FCT67–FCT72. [Google Scholar] [CrossRef]
- Blando, F.; Oomah, B.D. Sweet and sour cherries: Origin, distribution, nutritional composition and health benefits. Trends Food Sci. Technol. 2019, 86, 517–529. [Google Scholar] [CrossRef]
- Usenik, V.; Fajt, N.; Mikulic-Petkovsek, M.; Slatnar, A.; Stampar, F.; Veberic, R. Sweet Cherry Pomological and Biochemical Characteristics Influenced by Rootstock. J. Agric. Food Chem. 2010, 58, 4928–4933. [Google Scholar] [CrossRef]
- Kim, D.-O.; Heo, H.J.; Kim, Y.J.; Yang, H.S.; Lee, C.Y. Sweet and Sour Cherry Phenolics and Their Protective Effects on Neuronal Cells. J. Agric. Food Chem. 2005, 53, 9921–9927. [Google Scholar] [CrossRef]
- Średnicka-Tober, D.; Ponder, A.; Hallmann, E.; Głowacka, A.; Rozpara, E. The Profile and Content of Polyphenols and Carotenoids in Local and Commercial Sweet Cherry Fruits (Prunus avium L.) and Their Antioxid. Act. Vitro. Antioxid. 2019, 8, 534. [Google Scholar] [CrossRef]
- Ferretti, G.; Bacchetti, T.; Belleggia, A.; Neri, D. Cherry Antioxidants: From Farm to Table. Molecules 2010, 15, 6993–7005. [Google Scholar] [CrossRef] [PubMed]
- Overbeck, V.; Schmitz, M.; Blanke, M. Non-Destructive Sensor-Based Prediction of Maturity and Optimum Harvest Date of Sweet Cherry Fruit. Sensors 2017, 17, 277. [Google Scholar] [CrossRef] [PubMed]
- Abers, J.; Wrolstad, R. Causative factors of color deterioration in strawberry preserves during processing and storage. J. Food Sci. 1979, 44, 75–81. [Google Scholar] [CrossRef]
- Liu, Y.; Liu, X.; Zhong, F.; Tian, R.; Zhang, K.; Zhang, X.; Li, T. Comparative Study of Phenolic Compounds and Antioxidant Activity in Different Species of Cherries. J. Food Sci. 2011, 76, C633–C638. [Google Scholar] [CrossRef] [PubMed]
Figure 1.
Sweet cherry cultivars at the two fruit development phases examined.
Table 1.
Average monthly values of temperature, rainfall, and relative humidity during the experimental period (RSFG Iași, Romania, 2022–2023).
Table 1.
Average monthly values of temperature, rainfall, and relative humidity during the experimental period (RSFG Iași, Romania, 2022–2023).
| Month | Air Temperature (°C) a* | Precipitation (mm) L* | Relative Humidity (%) | |||
|---|---|---|---|---|---|---|
| 2022 | 2023 | 2022 | 2023 | 2022 | 2023 | |
| March | 3.2 | 6.6 | 56.9 | 5.8 | 60.0 | 58.0 |
| April | 10.0 | 9.7 | 58.0 | 2.0 | 59.1 | 66.0 |
| May | 16.6 | 16.2 | 17.4 | 5.0 | 50.5 | 54.0 |
| June | 21.9 | 20.7 | 26.6 | 10.0 | 53.6 | 53.0 |
| July | 23.2 | 23.3 | 27.8 | 91.5 | 50.0 | 51.0 |
Table 2.
Fruit skin colour of three sweet cherry cultivars at two development phases, examined in 2022.
Table 2.
Fruit skin colour of three sweet cherry cultivars at two development phases, examined in 2022.
| Treatment | Pre-Ripening Phase | Full Ripeness Phase | ||||
|---|---|---|---|---|---|---|
| L* | a* | b* | L* | a* | b* | |
| Cultivar | ||||||
| Van | 56.7 | 0.57 a | 38.1 | 20.4 b | 17.1 a | 1.7 b |
| Andreiaș | 56.7 | 0.43 b | 39.9 | 19.8 b | 15.7 a | 1.5 b |
| Margonia | 57.0 | 0.32 c | 40.7 | 60.8 a | 0.9 b | 35.3 a |
| n.s. | n.s. | |||||
| Fruit position | ||||||
| Inner | 58.7 | 0.59 | 41.2 | 33.6 | 12.0 | 13.3 |
| Outer | 54.9 | 0.28 | 37.9 | 33.8 | 10.4 | 12.4 |
| n.s. | * | n.s. | n.s. | * | n.s. | |
| Av. | 56.79 | 0.44 | 39.55 | 33.67 | 11.22 | 12.83 |
| STDEV | 2.15 | 0.25 | 2.25 | 21.01 | 8.20 | 17.43 |
| COVAR s% | 3.79 | 56.66 | 5.68 | 62.39 | 73.06 | 135.82 |
| Min. | 54.32 | 0.11 | 37.18 | 19.75 | 0.14 | 1.00 |
| Max. | 59.09 | 0.74 | 42.55 | 60.79 | 19.30 | 36.26 |
n.s.: not significant; * significant at p ≤ 0.05. Within each column, values followed by different letters are statistically different according to Duncan test at p ≤ 0.05, n = 3.
Table 3.
Fruit skin colour of three sweet cherry cultivars at two development phases, examined in 2023.
Table 3.
Fruit skin colour of three sweet cherry cultivars at two development phases, examined in 2023.
| Treatment | Pre-Ripening Phase | Full Ripeness Phase | ||||
|---|---|---|---|---|---|---|
| L* | a* | b* | L* | a* | b* | |
| Cultivar | ||||||
| Van | 57.5 a | 0.67 a | 38.5 a | 20.5 b | 17.5 a | 1.6 b |
| Andreiaș | 51.8 b | 0.35 b | 30.4 b | 20.7 b | 17.8 a | 2.0 b |
| Margonia | 58.2 a | 0.72 a | 38.3 a | 59.2 a | 1.9 b | 35.4 a |
| Fruit position | ||||||
| Inner | 56.3 | 0.59 | 36.0 | 33.6 | 12.4 | 13.4 |
| Outer | 55.3 | 0.56 | 35.4 | 33.3 | 12.3 | 12.6 |
| n.s. | n.s. | n.s. | n.s. | n.s. | n.s. | |
| Av. | 55.8 | 0.6 | 35.7 | 33.4 | 12.3 | 13.0 |
| STDEV | 3.3 | 0.3 | 4.2 | 19.5 | 8.1 | 17.3 |
| COVAR s% | 6.0 | 43.4 | 11.8 | 58.2 | 65.6 | 133.6 |
| Min. | 51.3 | 0.1 | 30.2 | 20.0 | 1.9 | 0.9 |
| Max. | 59.3 | 0.8 | 39.8 | 60.3 | 18.1 | 36.9 |
n.s.: not significant. Within each column, values followed by different letters are statistically different according to Duncan test at p ≤ 0.05, n = 3.
Table 4.
Dry matter and total soluble solids content of fruits from three sweet cherry cultivars at two development phases, examined in 2022.
Table 4.
Dry matter and total soluble solids content of fruits from three sweet cherry cultivars at two development phases, examined in 2022.
| Treatment | Pre-Ripening Phase | Full Ripeness Phase | ||
|---|---|---|---|---|
| DM (%) | TSS (°Brix) | DM (%) | TSS (°Brix) | |
| Cultivar | ||||
| Van | 13.1 a | 10.5 a | 21.4 a | 14.8 a |
| Andreiaș | 11.5 b | 9.5 b | 20.9 a | 12.4 b |
| Margonia | 11.9 b | 9.4 b | 18.0 b | 12.5 b |
| Fruit position inside the crown | ||||
| Inner | 11.6 | 9.5 | 19.9 | 13.5 |
| Outer | 12.8 | 10.1 | 20.2 | 13.0 |
| * | n.s. | n.s. | n.s. | |
| Av. | 12.17 | 9.80 | 20.06 | 13.23 |
| STDEV | 1.07 | 0.66 | 1.66 | 1.29 |
| COVAR s% | 8.81 | 6.77 | 8.29 | 9.71 |
| Min. | 10.78 | 9.03 | 17.93 | 11.90 |
| Max. | 13.30 | 10.81 | 21.80 | 14.80 |
TSS: total soluble solids; DM: dry matter content. n.s.: not significant; * significant at p ≤ 0.05. Within each column, values followed by different letters are statistically different according to Duncan test at p ≤ 0.05, n = 3.
Table 5.
Dry matter and total soluble solids content of fruits from three sweet cherry cultivars at two development phases, examined in 2023.
Table 5.
Dry matter and total soluble solids content of fruits from three sweet cherry cultivars at two development phases, examined in 2023.
| Treatment | Pre-Ripening Phase | Full Ripeness Phase | ||
|---|---|---|---|---|
| DM (%) | TSS (°Brix) | DM (%) | TSS (°Brix) | |
| Cultivar | ||||
| Van | 13.5 a | 10.8 a | 22.7 a | 15.3 a |
| Andreiaș | 11.5 b | 9.2 b | 20.5 b | 12.5 b |
| Margonia | 11.7 b | 9.5 b | 19.7 b | 12.7 b |
| Fruit position inside the crown | ||||
| Inner | 11.5 | 9.6 | 20.4 | 13.2 |
| Outer | 12.9 | 10.1 | 21.6 | 13.8 |
| * | n.s. | n.s. | n.s. | |
| Av. | 12.20 | 9.85 | 20.97 | 13.50 |
| STDEV | 1.27 | 1.12 | 1.53 | 1.58 |
| COVAR s% | 10.37 | 11.39 | 7.29 | 11.73 |
| Min. | 10.69 | 8.00 | 19.20 | 12.08 |
| Max. | 13.90 | 10.93 | 23.53 | 16.33 |
TSS: total soluble solids; DM: dry matter content. n.s.: not significant; * significant at p ≤ 0.05. Within each column, values followed by different letters are statistically different according to Duncan test at p ≤ 0.05, n = 3.
Table 6.
Biochemical parameters of fruits of three sweet cherry cultivars at two development phases, examined in 2022.
Table 6.
Biochemical parameters of fruits of three sweet cherry cultivars at two development phases, examined in 2022.
| Treatment | Pre-Ripening Phase | Full Ripeness Phase | ||||||
|---|---|---|---|---|---|---|---|---|
| pH | TA | TP | Vit C | pH | TA | TP | Vit C | |
| Cultivar | ||||||||
| Van | 3.83 | 1.12 | 124.1 c | 5.3 c | 3.76 | 0.95 | 77.2 a | 17.5 |
| Andreiaș | 4.02 | 1.05 | 156.2 b | 9.0 b | 3.85 | 0.92 | 65.6 b | 17.5 |
| Margonia | 4.03 | 1.15 | 186.9 a | 9.8 a | 3.85 | 0.87 | 63.8 b | 18.8 |
| n.s. | n.s. | n.s. | n.s. | n.s. | ||||
| Fruit position | ||||||||
| Inner | 3.96 | 1.14 | 156.8 | 7.7 | 3.80 | 0.92 | 75.4 | 17.0 |
| Outer | 3.95 | 1.07 | 154.8 | 8.3 | 3.84 | 0.91 | 62.3 | 18.8 |
| n.s. | n.s. | n.s. | n.s. | n.s. | n.s. | * | * | |
| Av. | 3.96 | 1.11 | 155.75 | 8.00 | 3.82 | 0.91 | 68.85 | 17.92 |
| STDEV | 0.10 | 0.08 | 28.82 | 2.28 | 0.06 | 0.04 | 11.70 | 1.28 |
| COVAR s% | 2.60 | 7.39 | 18.50 | 28.50 | 1.54 | 4.68 | 17.00 | 7.15 |
| Min. | 3.82 | 1.00 | 122.43 | 4.50 | 3.74 | 0.85 | 62.11 | 16.00 |
| Max. | 4.04 | 1.21 | 193.33 | 10.50 | 4.00 | 0.98 | 92.13 | 19.50 |
TA: titratable acidity, as mg malic acid 100 g−1 f.w.; TP: total phenolic content, as mg GAE 100 g−1 f.w.; vit C: vitamin C, as mg 100 g−1 f.w. n.s.: not significant; * significant at p ≤ 0.05. Within each column, values followed by different letters are statistically different according to Duncan test at p ≤ 0.05, n = 3.
Table 7.
Biochemical parameters of fruits of three sweet cherry cultivars at two development phases, examined in 2023.
Table 7.
Biochemical parameters of fruits of three sweet cherry cultivars at two development phases, examined in 2023.
| Treatment | Pre-Ripening Phase | Full Ripeness Phase | ||||||
|---|---|---|---|---|---|---|---|---|
| pH | TA | TP | Vit C | pH | TA | TP | Vit C | |
| Cultivar | ||||||||
| Van | 3.85 | 1.15 | 145.8 b | 5.5 c | 3.77 | 0.97 | 75.8 a | 17.3 b |
| Andreiaș | 3.71 | 1.12 | 152.8 b | 8.0 b | 3.90 | 1.01 | 60.0 b | 16.5 b |
| Margonia | 4.04 | 1.21 | 178.4 a | 10.0 a | 4.00 | 0.95 | 60.1 b | 20.3 a |
| n.s. | n.s. | n.s. | n.s. | |||||
| Fruit position | ||||||||
| Inner | 3.88 | 1.20 | 164.4 | 8.0 | 3.85 | 0.94 | 74.5 | 17.5 |
| Outer | 3.85 | 1.12 | 153.6 | 7.7 | 3.92 | 1.01 | 56.2 | 18.5 |
| n.s. | n.s. | n.s. | n.s. | n.s. | n.s. | * | n.s. | |
| Av. | 3.87 | 1.16 | 159.00 | 7.83 | 3.89 | 0.97 | 65.31 | 18.00 |
| STDEV | 0.16 | 0.08 | 17.02 | 2.75 | 0.12 | 0.06 | 14.33 | 2.51 |
| COVAR s% | 4.08 | 7.27 | 10.70 | 35.12 | 3.08 | 5.87 | 21.94 | 13.94 |
| Min. | 3.66 | 1.03 | 142.05 | 5.00 | 3.75 | 0.87 | 54.68 | 14.00 |
| Max. | 4.08 | 1.26 | 182.67 | 11.50 | 4.00 | 1.02 | 92.99 | 20.50 |
TA: titratable acidity, as mg malic acid 100 g−1 f.w.; TP: total phenolic content, as mg GAE 100 g−1 f.w.; vit C: vitamin C, as mg 100 g−1 f.w. n.s.: not significant; * significant at p ≤ 0.05. Within each column, values followed by different letters are statistically different according to Duncan test at p ≤ 0.05, n = 3.
Table 8.
Pigment content of fruits of three sweet cherry cultivars at two development phases, examined in 2022.
Table 8.
Pigment content of fruits of three sweet cherry cultivars at two development phases, examined in 2022.
| Treatment | Pre-Ripening Phase | Full Ripeness Phase | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Chl a | Chl b | Car | Lyc | Anth | Chl a | Chl b | Car | Lyc | Anth | |
| mg 100 g−1 f.w. | ||||||||||
| Cultivar | ||||||||||
| Van | 4.9 c | 9.3 c | 0.23 b | 0.06 b | 0.73 b | 1.45 c | 2.55 c | 0.11 b | 0.25 b | 21.6 b |
| Andreiaș | 17.7 b | 27.1 b | 0.45 a | 0.27 a | 1.24 a | 2.31 b | 5.20 b | 0.28 a | 0.35 a | 36.9 a |
| Margonia | 22.2 a | 36.5 a | 0.09 c | 0.09 b | 0.34 c | 3.11 a | 6.41 a | 0.28 a | 0.07 c | 4.7 c |
| Fruit position | ||||||||||
| Inner | 14.6 | 25.2 | 0.26 | 0.14 | 0.74 | 2.24 | 4.58 | 0.22 | 0.21 | 22.2 |
| Outer | 15.2 | 23.4 | 0.25 | 0.14 | 0.79 | 2.33 | 4.86 | 0.22 | 0.22 | 19.9 |
| n.s. | n.s. | n.s. | n.s. | n.s. | n.s. | n.s. | n.s. | n.s. | * | |
| Av. | 14.91 | 24.30 | 0.26 | 0.28 | 0.77 | 2,29 | 4.72 | 0.22 | 0.22 | 21.05 |
| STDEV | 8.06 | 12.63 | 0.17 | 0.33 | 0.44 | 0.75 | 1.78 | 0.09 | 0.13 | 14.58 |
| COVAR s% | 54.09 | 51.97 | 66.68 | 118.41 | 56.78 | 32.58 | 37.72 | 40.49 | 58.07 | 69.26 |
| Min. | 4.84 | 8.71 | 0.07 | 0.05 | 0.21 | 1.41 | 2.24 | 0.10 | 0.05 | 4.50 |
| Max. | 23.44 | 37.35 | 0.48 | 0.92 | 1.47 | 3.21 | 6.44 | 0.29 | 0.35 | 40.52 |
Chl a: chlorophyll a, as; Chl b: chlorophyll b; Car: carotene; Lyc: lycopene; Anth: anthocyanin. n.s.: not significant; * significant at p ≤ 0.05. Within each column, values followed by different letters are statistically different according to Duncan test at p ≤ 0.05, n = 3.
Table 9.
Pigment content of fruits of three sweet cherry cultivars at two development phases, examined in 2023.
Table 9.
Pigment content of fruits of three sweet cherry cultivars at two development phases, examined in 2023.
| Treatment | Pre-Ripening Phase | Full Ripeness Phase | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Chl a | Chl b | Car | Lyc | Anth | Chl a | Chl b | Car | Lyc | Anth | |
| mg 100 g−1 f.w. | ||||||||||
| Cultivar | ||||||||||
| Van | 5.9 c | 9.1 c | 0.29 b | 0.11 b | 0.87 b | 1.74 b | 3.65 b | 0.11 b | 0.08 c | 21.6 b |
| Andreiaș | 15.6 b | 25.8 b | 0.48 a | 0.35 a | 1.75 a | 4.65 a | 7.72 a | 0.36 a | 0.25 a | 39.4 a |
| Margonia | 21.8 a | 37.3 a | 0.10 c | 0.07 c | 0.46 c | 4.70 a | 7.12 a | 0.11 b | 0.16 b | 2.3 c |
| Fruit position | ||||||||||
| Inner | 15.0 | 25.1 | 0.29 | 0.16 | 0.90 | 3.54 | 5.65 | 0.19 | 0.15 | 22.8 |
| Outer | 13.8 | 23.0 | 0.29 | 0.19 | 1.15 | 3.85 | 6.68 | 0.20 | 0.17 | 19.4 |
| n.s. | n.s. | n.s. | * | * | * | n.s. | n.s. | * | * | |
| Av. | 14.42 | 24.04 | 0.29 | 0.17 | 1.03 | 3.69 | 6.16 | 0.19 | 0.16 | 21.09 |
| STDEV | 7.52 | 13.70 | 0.18 | 0.14 | 0.69 | 1.59 | 2.11 | 0.13 | 0.08 | 16.85 |
| COVAR s% | 52.17 | 56.98 | 63.59 | 78.23 | 67.18 | 43.13 | 34.22 | 66.94 | 46.69 | 79.90 |
| Min. | 5.86 | 8.77 | 0.08 | 0.10 | 0.40 | 1.01 | 2.52 | 0.10 | 0.10 | 2.14 |
| Max. | 23.27 | 41.03 | 0.49 | 0.35 | 2.30 | 4.90 | 7.73 | 0.37 | 0.26 | 44.19 |
Chl a: chlorophyll a; Chl b: chlorophyll b; Car: carotene; Lyc: lycopene; Anth: anthocyanin. n.s.: not significant; * significant at p ≤ 0.05. Within each column, values followed by different letters are statistically different according to Duncan test at p ≤ 0.05, n = 3.
Disclaimer/Publisher’s Note: The statements, opinions and data contained in all publications are solely those of the individual author(s) and contributor(s) and not of MDPI and/or the editor(s). MDPI and/or the editor(s) disclaim responsibility for any injury to people or property resulting from any ideas, methods, instructions or products referred to in the content. |
© 2024 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
Share and Cite
MDPI and ACS Style
Mineață, I.; Murariu, O.C.; Sîrbu, S.; Tallarita, A.V.; Caruso, G.; Jităreanu, C.D. Effects of Ripening Phase and Cultivar under Sustainable Management on Fruit Quality and Antioxidants of Sweet Cherry. Horticulturae 2024, 10, 720. https://doi.org/10.3390/horticulturae10070720
AMA Style
Mineață I, Murariu OC, Sîrbu S, Tallarita AV, Caruso G, Jităreanu CD. Effects of Ripening Phase and Cultivar under Sustainable Management on Fruit Quality and Antioxidants of Sweet Cherry. Horticulturae. 2024; 10(7):720. https://doi.org/10.3390/horticulturae10070720
Chicago/Turabian StyleMineață, Iulia, Otilia Cristina Murariu, Sorina Sîrbu, Alessio Vincenzo Tallarita, Gianluca Caruso, and Carmen Doina Jităreanu. 2024. "Effects of Ripening Phase and Cultivar under Sustainable Management on Fruit Quality and Antioxidants of Sweet Cherry" Horticulturae 10, no. 7: 720. https://doi.org/10.3390/horticulturae10070720
Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.
